| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,380,360 | (C)5→6 | coding (284/1296 nt) | menF ← | isochorismate synthase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,380,355 | 1 | . | C | 96.8% | 108.6 / ‑3.0 | 31 | coding (289/1296 nt) | menF | isochorismate synthase 2 |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (14/16); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
ATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAG‑CCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAAT > NC_000913/2380217‑2380488 | aTTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAg < 2:316233/139‑1 (MQ=255) gCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCGGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAG‑CCCCCcaa < 2:278088/139‑1 (MQ=255) gCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAG‑ccccccaa < 1:271544/139‑4 (MQ=255) cATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGt > 1:301988/1‑139 (MQ=255) gCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTg < 1:884732/139‑1 (MQ=255) ttCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTg < 1:1108192/139‑1 (MQ=255) aTAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCgaagaa > 2:258606/1‑139 (MQ=255) tAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGaaa < 1:498389/139‑1 (MQ=255) cGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACg < 1:655962/139‑1 (MQ=255) cGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACg > 2:655962/1‑139 (MQ=255) gTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGt > 1:1123279/1‑139 (MQ=255) tCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGtt < 2:1123279/139‑1 (MQ=255) gCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAg > 2:684720/1‑102 (MQ=255) gCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAg < 1:684720/102‑1 (MQ=255) ccGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGc < 2:478201/139‑1 (MQ=255) aGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTgg > 1:80008/1‑139 (MQ=255) ggCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCaa < 1:154126/139‑1 (MQ=255) ccGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGc < 1:376041/90‑1 (MQ=255) ccGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGc > 2:376041/1‑90 (MQ=255) ccGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTa > 1:1004398/1‑139 (MQ=255) cAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAg < 1:258606/139‑1 (MQ=255) ggggTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAg > 1:943504/1‑43 (MQ=255) ggggTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAg < 2:943504/43‑1 (MQ=255) gTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATc > 2:1229672/1‑123 (MQ=255) gTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATc < 1:1229672/123‑1 (MQ=255) tAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGc < 2:49427/139‑1 (MQ=255) aaTTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCga < 1:161781/72‑1 (MQ=255) aaTTGCCCTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCga > 2:161781/1‑72 (MQ=255) cccTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCa > 2:1046089/1‑139 (MQ=255) cTGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCAtt < 2:88683/139‑1 (MQ=255) tGCGACGGGTCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTa > 1:582632/1‑139 (MQ=255) tCAAAAGCATTCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGc < 2:1004398/139‑1 (MQ=255) ttCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAAt > 1:314732/1‑139 (MQ=255) ttCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGATAATCGCGCCCAGGACGACAGCTTCTtcatca < 1:832983/115‑1 (MQ=255) ttCAGCCCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGATAATCGCGCCCAGGACGACAGCTTCTtcatca > 2:832983/1‑115 (MQ=255) | ATTCTTTTGCCTGAATCGCATCGTGCTGAAGGGAGCTTTCGCTGAATAGCGTCAGCCGCAGCGTGGCTTTACCGCCACAGCGTCGCCATTCCAGGCGGGGTAAAAGTAAATTGCCCTGCGACGGGTCAAAAGCATTCAG‑CCCCCAAATGCGTAAGTCGGCGTGTTCCGGGTGCTGGCGAAGAAAACGTTGTGCCTGGTCCAACGACGTAAAACGGGTAATCGCGCCCAGGACGACAGCTTCTTCATCACCATTACGTTGTTGCCAGTAGAAT > NC_000913/2380217‑2380488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |