Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,271,600 (C)5→6 coding (171/1146 nt) garK ← glycerate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,271,5951.C100.0% 84.1 / NA 24A59G (GCT→GGT) garKglycerate kinase I
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (12/12);  total (12/12)

TCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCG  >  NC_000913/3271457‑3271720
                                                                                                                                           |                                                                                                                             
tCGCGTTTTTCCGCCGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTATTTCCATCGCCGGAGATCCCCCAATTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAg                                                                                                                                <  1:758813/139‑1 (MQ=255)
 cgcgTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGc                                                                                                                              <  1:1032729/139‑1 (MQ=255)
                       cTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAatcat                                                                                                         <  1:652483/139‑1 (MQ=255)
                                  tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCg                                                                                              >  2:71116/1‑139 (MQ=255)
                                  tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCg                                                                                              >  2:569424/1‑139 (MQ=255)
                                                        cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAc                                                                        <  2:811150/139‑1 (MQ=255)
                                                        cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAc                                                                        >  1:811150/1‑139 (MQ=255)
                                                           ggTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCgg                                                                     <  2:1221234/139‑1 (MQ=255)
                                                            gTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGa                                                                    >  2:218777/1‑139 (MQ=255)
                                                            gTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGa                                                                    >  2:208935/1‑139 (MQ=255)
                                                             ttttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCGGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGaa                                                                   <  1:1144138/139‑1 (MQ=255)
                                                                       gCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                                                         >  2:408205/1‑139 (MQ=255)
                                                                                          gCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAtt                                      >  2:810616/1‑139 (MQ=255)
                                                                                          gCATTCACTTTCTCGCCCAGCGACCCTGAAACCCTGGCGTGACGTTCAGCCCCCCTGGGTGCCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAtt                                      >  2:800662/1‑139 (MQ=255)
                                                                                             ttCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAatcat                                                                                                         >  2:1145076/1‑69 (MQ=255)
                                                                                             ttCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAatcat                                                                                                         <  1:1145076/69‑1 (MQ=255)
                                                                                                  tttCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGa                                <  1:887107/137‑1 (MQ=255)
                                                                                                  tttCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTGCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGa                                >  2:887107/1‑137 (MQ=255)
                                                                                                   ttCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAAt                             <  1:71116/139‑1 (MQ=255)
                                                                                                    tctcGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATc                            <  1:810616/139‑1 (MQ=255)
                                                                                                      tcGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCt                          <  1:703397/139‑1 (MQ=255)
                                                                                                        gCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAc                                                                        <  2:386908/91‑1 (MQ=255)
                                                                                                        gCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAAc                                                                        >  1:386908/1‑91 (MQ=255)
                                                                                                                 cccTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGc               <  2:670978/139‑1 (MQ=255)
                                                                                                                        aCCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCa        <  1:218777/139‑1 (MQ=255)
                                                                                                                              gCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCg  >  1:137005/1‑139 (MQ=255)
                                                                                                                                           |                                                                                                                             
TCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCG  >  NC_000913/3271457‑3271720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: