Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,479,980	(T)7→8	coding (251/1197 nt)	yjgN →	DUF898 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,479,973	1	.	T	100.0%	193.3 / NA	55	V82F (GTT→TTT)	yjgN	DUF898 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (32/23);  total (32/23)

TAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTG‑TTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGC  >  NC_000913/4479845‑4480097                                                                                                                                  |                                                                                                                             tAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTG‑ttttttttct                                                                                                                    >  2:125496/1‑136 (MQ=255)              aCCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCa                                                                                                             >  1:871275/1‑134 (MQ=255)              aCCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCa                                                                                                             <  2:871275/134‑1 (MQ=255)              aCCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCtt                                                                                                        >  1:85429/1‑139 (MQ=255)                 aTGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAAt                                                                                                     >  1:1139546/1‑139 (MQ=255)                    ggCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAGGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGAc                                                                                                  >  1:809101/1‑139 (MQ=255)                    ggCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGAc                                                                                                  >  2:325270/1‑139 (MQ=255)                     gCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTg                                                                                                                               >  2:38266/1‑109 (MQ=255)                     gCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTg                                                                                                                               <  1:38266/109‑1 (MQ=255)                      cATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAg                                                                                                <  2:1172557/139‑1 (MQ=255)                                     aGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCa                                                                                                             >  2:1230639/1‑111 (MQ=255)                                     aGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCa                                                                                                             <  1:1230639/111‑1 (MQ=255)                                     aGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGc                                                                                 >  2:387371/1‑139 (MQ=255)                                     aGCGTTATCTTTATGCTAAAATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGc                                                                                 <  2:809101/139‑1 (MQ=255)                                           aTCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCttg                                                                           >  2:555436/1‑139 (MQ=255)                                                 aTGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTgttggtt                                                                     >  1:606166/1‑139 (MQ=255)                                                    cTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTgttggt                                                                      <  1:143675/135‑1 (MQ=255)                                                    cTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTgttggt                                                                      >  2:143675/1‑135 (MQ=255)                                                      aaTATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCg                                                                                                               <  1:326076/92‑1 (MQ=255)                                                      aaTATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCg                                                                                                               >  2:326076/1‑92 (MQ=255)                                                      aaTATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGtt                                                                >  2:888530/1‑139 (MQ=255)                                                      aaTATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGtt                                                                >  1:786304/1‑139 (MQ=255)                                                      aaTATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGtt                                                                <  1:387371/139‑1 (MQ=255)                                                       atatGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGAc                                                                                                  <  1:1203425/104‑1 (MQ=255)                                                       atatGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGAc                                                                                                  >  2:1203425/1‑104 (MQ=255)                                                       atatGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGttt                                                               >  1:290061/1‑139 (MQ=255)                                                       atatGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATC‑TAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGtttt                                                              >  1:811402/1‑139 (MQ=255)                                                          tGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGa                                                            <  2:606166/139‑1 (MQ=255)                                                               gTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTg                                                       >  2:1165370/1‑139 (MQ=255)                                                                ttAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGt                                                      <  2:85429/139‑1 (MQ=255)                                                                ttAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGt                                                      <  1:1165370/139‑1 (MQ=255)                                                                  aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCa                                                                                                             >  1:554034/1‑82 (MQ=255)                                                                  aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCa                                                                                                             <  2:554034/82‑1 (MQ=255)                                                                  aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCttgt                                                                          <  2:948734/117‑1 (MQ=255)                                                                  aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCttgt                                                                          >  1:948734/1‑117 (MQ=255)                                                                  aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTg                                                       <  1:296617/136‑1 (MQ=255)                                                                  aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTg                                                       >  2:296617/1‑136 (MQ=255)                                                                  aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTa                                                    >  1:586983/1‑139 (MQ=255)                                                                    cGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACt                                                  >  1:1126461/1‑139 (MQ=255)                                                                          aCGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACt                                                          >  2:399040/1‑125 (MQ=255)                                                                          aCGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACt                                                          <  1:399040/125‑1 (MQ=255)                                                                               tttCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGc                                       <  2:764536/139‑1 (MQ=255)                                                                                      tGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATAtt                                >  1:845574/1‑139 (MQ=255)                                                                                                   gggAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCaaaa                   >  1:38575/1‑139 (MQ=255)                                                                                                         gTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTg             >  1:71091/1‑139 (MQ=255)                                                                                                          tttttGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGc            <  2:1139546/139‑1 (MQ=255)                                                                                                              tGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTg                                                       <  2:194930/92‑1 (MQ=255)                                                                                                              tGTTAGTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTg                                                       >  1:194930/1‑92 (MQ=255)                                                                                                                   gTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAgg   <  1:529103/139‑1 (MQ=255)                                                                                                                   gTTGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAgg   <  2:290061/139‑1 (MQ=255)                                                                                                                    ttGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGt                                            <  2:852921/97‑1 (MQ=255)                                                                                                                    ttGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGt                                            >  1:852921/1‑97 (MQ=255)                                                                                                                    ttGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCtt                                     <  1:685058/104‑1 (MQ=255)                                                                                                                    ttGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCtt                                     >  2:685058/1‑104 (MQ=255)                                                                                                                    ttGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGc  >  1:281914/1‑139 (MQ=255)                                                                                                                    ttGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGc  >  1:10989/1‑139 (MQ=255)                                                                                                                     tGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGtcat       >  2:24471/1‑133 (MQ=255)                                                                                                                     tGTCTTTTTTTTGTTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGtcat       <  1:24471/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                             TAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTG‑TTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGC  >  NC_000913/4479845‑4480097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑