Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,929	G→A	W193* (TGG→TGA)	yabI →	DedA family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,929	0	G	A	100.0%	118.3 / NA	35	W193* (TGG→TGA)	yabI	DedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/19);  total (16/19)

CCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTG  >  NC_000913/71794‑72049                                                                                                                                        |                                                                                                                         cctgcTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGgctgactg                                                                                                                       >  2:114404/1‑139 (MQ=255)   tgctgTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAGTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACtgtg                                                                                                                     <  1:619157/139‑1 (MQ=255)   tgctgTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACtgtg                                                                                                                     <  2:949317/139‑1 (MQ=255)          gccgccGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCgg                                                                                                              >  1:791809/1‑139 (MQ=255)                  tttACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGgcg                                                                                                      >  1:1118028/1‑139 (MQ=255)                      cTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGc                                                                                                  <  2:988015/139‑1 (MQ=255)                      cTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGc                                                                                                  <  2:134309/139‑1 (MQ=255)                         ccTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGt                                                                                               >  2:176312/1‑139 (MQ=255)                            gCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTaaa                                                                                            >  1:258319/1‑139 (MQ=255)                                    ttCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGa                                                                                    <  1:497775/139‑1 (MQ=255)                                         gcgggcgCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGtt                                                                               <  2:1118028/139‑1 (MQ=255)                                            ggcgCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGa                                                                            >  1:375574/1‑139 (MQ=255)                                                              ccTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCcgcg                                                          <  1:318393/139‑1 (MQ=255)                                                                tGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGt                                                        >  1:540365/1‑139 (MQ=255)                                                                 gCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTc                                                                         >  1:369041/1‑121 (MQ=255)                                                                 gCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTc                                                                         <  2:369041/121‑1 (MQ=255)                                                                    ggAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCt                                                                                                                   >  2:958055/1‑76 (MQ=255)                                                                    ggAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCt                                                                                                                   <  1:958055/76‑1 (MQ=255)                                                                    ggAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGa                                                                                        <  2:921257/103‑1 (MQ=255)                                                                    ggAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGa                                                                                        >  1:921257/1‑103 (MQ=255)                                                                    ggAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTcc                                                              >  1:484816/1‑129 (MQ=255)                                                                    ggAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTcc                                                              <  2:484816/129‑1 (MQ=255)                                                                               cGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTg                                         >  2:539580/1‑139 (MQ=255)                                                                                    aGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGcc                                    >  2:1131441/1‑139 (MQ=255)                                                                                     gTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCg                                   <  2:258319/139‑1 (MQ=255)                                                                                              gTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGATATGGCGCAGCGGTAAAGCGACTGACCGttt                                                                              <  1:1227374/87‑1 (MQ=255)                                                                                              gTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGATATGGCGCAGCGGTAAAGCGACTGACCGttt                                                                              >  2:1227374/1‑87 (MQ=255)                                                                                                 gctgctGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTGGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGcc                       >  2:330026/1‑139 (MQ=255)                                                                                                   tgctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAt                     <  1:330026/139‑1 (MQ=255)                                                                                                                  tgtTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCt      >  2:498009/1‑139 (MQ=255)                                                                                                                   gtTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtg     <  1:756084/139‑1 (MQ=255)                                                                                                                   gtTTTTGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtg     <  1:539580/139‑1 (MQ=255)                                                                                                                    tttttGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtgg    <  1:498009/139‑1 (MQ=255)                                                                                                                      tttGTGGGTTGGTGGGTGACTGTGCTGGCGGTTGTGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCTTTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggtg  <  2:36392/139‑1 (MQ=255)                                                                                                                       ttGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCgg                  <  1:520799/122‑1 (MQ=255)                                                                                                                       ttGTGGGTTGGTGGCTGACTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGACATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCgg                  >  2:520799/1‑122 (MQ=255)                                                                                                                                        |                                                                                                                         CCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTG  >  NC_000913/71794‑72049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑