Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,241,732	G→A	L114L (CTG→TTG)	cvrA ←	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,241,732	0	G	A	100.0%	110.0 / NA	33	L114L (CTG→TTG)	cvrA	putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/16);  total (17/16)

CCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCC  >  NC_000913/1241596‑1241865                                                                                                                                         |                                                                                                                                      ccGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAAcc                                                                                                                                     >  1:1091390/1‑139 (MQ=255)     aTTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCAcgc                                                                                                                                 >  1:839458/1‑139 (MQ=255)                 gCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGcc                                                                                                                     >  2:574437/1‑139 (MQ=255)                    tCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGt                                                                                                                  <  1:852477/139‑1 (MQ=255)                         ccAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAAc                                                                                                             <  2:57837/139‑1 (MQ=255)                         ccAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAAc                                                                                                             <  1:975368/139‑1 (MQ=255)                           aaCACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTCAAACCa                                                                                                           <  2:928221/139‑1 (MQ=255)                                      tAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAg                                                                                                <  2:647427/139‑1 (MQ=255)                                              ttACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCa                                                                                        >  1:424477/1‑139 (MQ=255)                                               tACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATg                                                                                                                       <  1:209845/107‑1 (MQ=255)                                               tACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATg                                                                                                                       >  2:209845/1‑107 (MQ=255)                                                         cagAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGc                                                                             >  2:101034/1‑139 (MQ=255)                                                              aaaaGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATg                                                                                                                       >  1:306157/1‑92 (MQ=255)                                                              aaaaGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATg                                                                                                                       <  2:306157/92‑1 (MQ=255)                                                                      gCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGAc                                                                          >  2:155874/1‑129 (MQ=255)                                                                      gCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGAc                                                                          <  1:155874/129‑1 (MQ=255)                                                                                  gTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGc                                                                                      <  2:545240/105‑1 (MQ=255)                                                                                  gTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGc                                                                                      >  1:545240/1‑105 (MQ=255)                                                                                     gagCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGaa                                                 <  1:574437/139‑1 (MQ=255)                                                                                      agCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCa                                                                                                           <  1:309135/80‑1 (MQ=255)                                                                                      agCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCa                                                                                                           >  2:309135/1‑80 (MQ=255)                                                                                          gacgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGa                                             <  1:919053/138‑1 (MQ=255)                                                                                          gacgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGa                                             >  2:919053/1‑138 (MQ=255)                                                                                                aGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGcc                                      <  2:839458/139‑1 (MQ=255)                                                                                                                  ttCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCccgccg                    >  1:312998/1‑139 (MQ=255)                                                                                                                  ttCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCccgccg                    >  1:957938/1‑139 (MQ=255)                                                                                                                  ttCAATCAAATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGAGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCccgccg                    >  2:676222/1‑139 (MQ=255)                                                                                                                   tcaatcaaATCAAGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGt                   >  1:1023382/1‑139 (MQ=255)                                                                                                                      atcaaatcaaGATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGa                <  2:957938/139‑1 (MQ=255)                                                                                                                                gATTAAACAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAaataat      >  2:43218/1‑139 (MQ=255)                                                                                                                                    aaaCAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGcc  <  2:424477/139‑1 (MQ=255)                                                                                                                                     aacaacCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCcac                                                      <  1:994477/86‑1 (MQ=255)                                                                                                                                     aacaacCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCcac                                                      >  2:994477/1‑86 (MQ=255)                                                                                                                                      aCAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGa                                             >  2:862986/1‑94 (MQ=255)                                                                                                                                      aCAACCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGa                                             <  1:862986/94‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CCGGATTCAATTTCCAGCGTCGAGCCAACACGTTCGTTAAGCCCCTTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCC  >  NC_000913/1241596‑1241865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑