Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,259,697 C→T P369P (CCG→CCA)  dauA ← C4‑dicarboxylic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,259,6970CT100.0% 157.2 / NA 46P369P (CCG→CCA) dauAC4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/27);  total (19/27)

TGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGG  >  NC_000913/1259573‑1259831
                                                                                                                            |                                                                                                                                      
tGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACcag                                                                                                                          <  2:619787/139‑1 (MQ=255)
tGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACcag                                                                                                                          >  2:1163311/1‑139 (MQ=255)
 gACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACcagc                                                                                                                         <  2:1226502/139‑1 (MQ=255)
          gTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGa                                                                                                                <  2:998088/139‑1 (MQ=255)
          gTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGa                                                                                                                >  1:818674/1‑139 (MQ=255)
               cTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAAc                                                                                                           <  2:182767/139‑1 (MQ=255)
               cTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAAc                                                                                                           <  2:1241116/139‑1 (MQ=255)
                 ttCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCa                                                                                                         <  2:429253/139‑1 (MQ=255)
                              gcagcaAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGAt                                                                                            <  2:323500/139‑1 (MQ=255)
                                     gTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCAccgcc                                                                                     >  1:249560/1‑139 (MQ=255)
                                     gTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCAccgcc                                                                                     <  2:647929/139‑1 (MQ=255)
                                            aCTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAg                                                                              >  2:813858/1‑139 (MQ=255)
                                                       ttCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGAt                                                                                            >  2:1156371/1‑114 (MQ=255)
                                                       ttCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGAt                                                                                            <  1:1156371/114‑1 (MQ=255)
                                                       ttCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGcc                                                                   <  2:249560/139‑1 (MQ=255)
                                                            tCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCAccgcc                                                                                     <  1:417641/116‑1 (MQ=255)
                                                            tCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCAccgcc                                                                                     >  2:417641/1‑116 (MQ=255)
                                                            tCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGc                                                              <  1:28326/139‑1 (MQ=255)
                                                               tGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACg                                                           <  1:813858/139‑1 (MQ=255)
                                                                 ttCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGa                                                         >  1:56288/1‑139 (MQ=255)
                                                                   ccACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACg                                                       >  1:270463/1‑139 (MQ=255)
                                                                      cGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTa                                                    >  1:416290/1‑139 (MQ=255)
                                                                           ccaTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGGGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGc                                               >  1:333932/1‑139 (MQ=255)
                                                                           ccaTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACgg                                                          >  1:808007/1‑128 (MQ=255)
                                                                           ccaTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACgg                                                          <  2:808007/128‑1 (MQ=255)
                                                                            caTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATaa                                                                                                            <  1:252330/77‑1 (MQ=255)
                                                                            caTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATaa                                                                                                            >  2:252330/1‑77 (MQ=255)
                                                                              tCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAggg                                                                                                                     >  1:871442/1‑66 (MQ=255)
                                                                              tCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAggg                                                                                                                     <  2:871442/66‑1 (MQ=255)
                                                                                aaCAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAAc                                          >  1:176681/1‑139 (MQ=255)
                                                                                    gcagGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAAcgcgc                                      <  2:416290/139‑1 (MQ=255)
                                                                                       gggCTGCCATGGCGGAAAGCGGCAGCCAGGAGGGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGAt                                   <  2:333932/139‑1 (MQ=255)
                                                                                        ggCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAAt                                                                                                              <  2:635509/63‑1 (MQ=255)
                                                                                        ggCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAAt                                                                                                              >  1:635509/1‑63 (MQ=255)
                                                                                        ggCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGc                                                              <  2:1099040/111‑1 (MQ=255)
                                                                                        ggCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGc                                                              >  1:1099040/1‑111 (MQ=255)
                                                                                          cTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGa                                                                                                                <  2:52181/59‑1 (MQ=255)
                                                                                          cTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGa                                                                                                                >  1:52181/1‑59 (MQ=255)
                                                                                          cTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGc                                >  1:198193/1‑139 (MQ=255)
                                                                                          cTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGc                                <  1:1099353/139‑1 (MQ=255)
                                                                                                tGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGt                          <  2:270463/139‑1 (MQ=255)
                                                                                                tGGCGGAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGt                          <  1:865003/139‑1 (MQ=255)
                                                                                                    ggAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCAccgcc                                                                                     <  2:1198583/76‑1 (MQ=255)
                                                                                                    ggAAAGCGGCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCAccgcc                                                                                     >  1:1198583/1‑76 (MQ=255)
                                                                                                           ggCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATac               <  2:56288/139‑1 (MQ=255)
                                                                                                           ggCAGCCAGGAGAGCAGTGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATac               <  1:908772/139‑1 (MQ=255)
                                                                                                                        gcagtGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACgg  <  1:1163311/139‑1 (MQ=255)
                                                                                                                            |                                                                                                                                      
TGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGG  >  NC_000913/1259573‑1259831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑