Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,278,189	C→T	T78I (ACT→ATT)	narK →	nitrate/nitrite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,278,189	0	C	T	100.0%	139.9 / NA	41	T78I (ACT→ATT)	narK	nitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/24);  total (17/24)

TGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGACTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTATGGTTCCTATCTTCGGTGGTCGTCGCTGGACGGCGTTCAGCACCGGTATTCTGATTATTCCTTGCGTCTGGCTGGGTTTT  >  NC_000913/1278055‑1278319                                                                                                                                       |                                                                                                                                   tGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTgcg                                                                                                                                >  2:48159/1‑139 (MQ=255) tGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTgcg                                                                                                                                <  2:135679/139‑1 (MQ=255)   ccAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCt                                                                                                                              <  1:417369/139‑1 (MQ=255)      gCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGcc                                                                                                                           >  2:989586/1‑139 (MQ=255)      gCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGcc                                                                                                                           >  2:25865/1‑139 (MQ=255)        cGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCtt                                                                                                                         >  2:545968/1‑139 (MQ=255)        cGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCtt                                                                                                                         >  2:236184/1‑139 (MQ=255)                    aTTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGgcg                                                                                                             <  1:48159/139‑1 (MQ=255)                           ttCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGgcgc                                                                                                            <  1:714231/133‑1 (MQ=255)                           ttCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGttatt                                                                                                      >  1:1135861/1‑139 (MQ=255)                           ttCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGttatt                                                                                                      <  1:25865/139‑1 (MQ=255)                           ttCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGttatt                                                                                                      >  1:410221/1‑139 (MQ=255)                           ttCCCTGTCTGCTGCTGGCGTTTTGCGTAGGGATGTTGTTCAGCGATGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGgcgc                                                                                                            >  2:714231/1‑133 (MQ=255)                             cccTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTAc                                                                                                    <  2:410221/139‑1 (MQ=255)                                tGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTACTTTTACGACCGGTCAGCTATTTATGTTGATTGCGCTGCCTGCGGTTTCTGGCGCGTTATTACgtg                                                                                                 <  2:806637/139‑1 (MQ=255)                                tGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACgtg                                                                                                 <  1:494766/139‑1 (MQ=255)                                tGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACgtg                                                                                                 <  2:563289/139‑1 (MQ=255)                                tGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACgtg                                                                                                 <  2:505641/139‑1 (MQ=255)                                  tCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGtt                                                                                               >  2:702525/1‑139 (MQ=255)                                       tgctgGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATa                                                                                          <  1:545968/139‑1 (MQ=255)                                       tgctgGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATa                                                                                          <  1:702525/139‑1 (MQ=255)                                       tgctgGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATa                                                                                          <  1:47132/139‑1 (MQ=255)                                        gctgGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGgc                                                                                                              >  1:1020221/1‑118 (MQ=255)                                        gctgGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGgc                                                                                                              <  2:1020221/118‑1 (MQ=255)                                               ttttGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACTTACCGAAAGTCGGCTTTAATTTTACGTCCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGGTCCATACTCCTTTa                                                                                  <  1:751405/139‑1 (MQ=255)                                               ttttGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTa                                                                                  <  2:758941/139‑1 (MQ=255)                                               ttttGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTa                                                                                  <  1:989586/139‑1 (MQ=255)                                               ttttGCGTATGGATGTTCTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTATACGACCGATCAGCTATTTATGTTGATTGCGCTGCTTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCtttg                                                                                  <  1:761304/139‑2 (MQ=255)                                                                                     ccTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTATGGTTCCTATCTTCGGTGGTCGTCGCTGGACGGCGTTc                                            >  2:919493/1‑139 (MQ=255)                                                                                        aCCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGttatt                                                                                                      <  1:705573/78‑1 (MQ=255)                                                                                        aCCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGttatt                                                                                                      >  2:705573/1‑78 (MQ=255)                                                                                        aCCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTAt                                                                                 <  2:377258/99‑1 (MQ=255)                                                                                        aCCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTAt                                                                                 >  1:377258/1‑99 (MQ=255)                                                                                          cGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTATGGTTCCTATCTTCGGTGGTCGTCGCTGGACGGCGTTCAGCAc                                       <  1:236184/139‑1 (MQ=255)                                                                                               gTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTgg                                                                                                               >  1:940384/1‑62 (MQ=255)                                                                                               gTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTgg                                                                                                               <  2:940384/62‑1 (MQ=255)                                                                                                tCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTTTTCCATACTCCTTTATGGTTCCTATCTTCGGTGGTCGTCGCTGGACGGCGTTCAGCACCGGTAt                                 <  2:1135861/139‑1 (MQ=255)                                                                                                   gCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGgcg                                                                                                             >  2:467942/1‑60 (MQ=255)                                                                                                   gCTTTAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGgcg                                                                                                             <  1:467942/60‑1 (MQ=255)                                                                                                       tAATTTTACGACCGATCAGCTATTTATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTATGGTTCCTATCTTCGGTGGTCGTCGCTGGACGGCGTTCAGCACCGGTATTCTGatt                          >  1:71679/1‑139 (MQ=255)                                                                                                                               tATGTTGATTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTATGGTTCCTATCTTCGGTGGTCGTCGCTGGACGGCGTTCAGCACCGGTATTCTGATTATTCCTTGCGTCTGGCTGGGtttt  >  2:921620/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                   TGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTGAACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGACTGCGCTGCCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTATGGTTCCTATCTTCGGTGGTCGTCGCTGGACGGCGTTCAGCACCGGTATTCTGATTATTCCTTGCGTCTGGCTGGGTTTT  >  NC_000913/1278055‑1278319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑