| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,287,826 | C→T | A267T (GCA→ACA) | purU ← | formyltetrahydrofolate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,287,826 | 0 | C | T | 98.3% | 206.1 / NA | 60 | A267T (GCA→ACA) | purU | formyltetrahydrofolate hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (27/32); minor base A (0/1); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAT > NC_000913/1287690‑1287957 | gccgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa < 2:347057/139‑1 (MQ=255)gccgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa < 1:925615/139‑1 (MQ=255)gccgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa < 1:576032/139‑1 (MQ=255) ttACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGt > 1:1095593/1‑139 (MQ=255) cGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCa > 2:556037/1‑139 (MQ=255) agaAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACt > 2:350280/1‑139 (MQ=255) agaAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACt > 1:947078/1‑139 (MQ=255) agaAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACt > 2:395761/1‑139 (MQ=255) tGAACTGTTGGCGTAATTTGCATCCGTAACGGATTAAAGGTAACCAGTTATTTTGGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACg < 1:1034085/139‑1 (MQ=255) aCTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGttt > 1:1063498/1‑139 (MQ=255) aCTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGttt > 2:1227342/1‑139 (MQ=255) cTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGtttt > 2:796679/1‑139 (MQ=255) ttGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTtctc > 2:1199467/1‑139 (MQ=255) tGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCg < 1:350280/139‑1 (MQ=255) tGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCg < 1:22320/139‑1 (MQ=255) tGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCg < 1:1149185/139‑1 (MQ=255) cGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg < 2:1063498/139‑1 (MQ=255) gTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcgc < 1:1227342/139‑1 (MQ=255) ggATTAAAGGTAACTAGATATTTTTGCTGGCGATTAAACAATAAGCGTTCGAGTACCGAAAACAAAGACGCGCTGTGACAGTACTTTGTATAGTGCACGACTTAGCACTGTTTTCTCGACATCACGACCTGCGTGcatc < 2:873946/139‑1 (MQ=255) ggATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGcatc < 2:416469/139‑1 (MQ=255) ggATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGcatc < 1:956980/139‑1 (MQ=255) aTTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGcatcat < 2:387496/139‑1 (MQ=255) tAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg < 2:1175485/139‑1 (MQ=255) tAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg < 2:1095593/139‑1 (MQ=255) tAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg < 1:755152/139‑1 (MQ=255) tAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg < 1:1199467/139‑1 (MQ=255) aaCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt < 2:703996/71‑1 (MQ=255) aaCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt > 1:703996/1‑71 (MQ=255) aaCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGc > 2:664731/1‑139 (MQ=255) aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt > 1:643376/1‑70 (MQ=255) aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt < 2:643376/70‑1 (MQ=255) aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCg > 2:249760/1‑106 (MQ=255) aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCg < 1:249760/106‑1 (MQ=255) aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCt > 1:1164630/1‑139 (MQ=255) aGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgtg < 1:337159/139‑1 (MQ=255) ttATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTa > 2:377868/1‑139 (MQ=255) aTTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCa < 1:612495/78‑1 (MQ=255) aTTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCa > 2:612495/1‑78 (MQ=255) cTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATc < 1:377868/139‑1 (MQ=255) cGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgtg > 2:1061763/1‑124 (MQ=255) cGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgtg < 1:1061763/124‑1 (MQ=255) aaaGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAAt < 2:1011006/139‑1 (MQ=255) taatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg > 2:160782/1‑92 (MQ=255) taatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg < 1:160782/92‑1 (MQ=255) taatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTc < 2:947078/139‑1 (MQ=255) atCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCt > 2:951779/1‑139 (MQ=255) tCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACg > 2:298236/1‑134 (MQ=255) tCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACg < 1:298236/134‑1 (MQ=255) tCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatg < 1:796679/139‑1 (MQ=255) aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTc > 2:1215701/1‑139 (MQ=255) aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTc > 1:528196/1‑139 (MQ=255) aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTc > 1:1225686/1‑139 (MQ=255) gcgcTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGa < 1:556037/139‑1 (MQ=255) cgcTGTGTCAGTACTTTGTATAGTGCACGACTTaa < 2:465671/35‑1 (MQ=255) cgcTGTGTCAGTACTTTGTATAGTGCACGACTTaa > 1:465671/1‑35 (MQ=255) cgcTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAt > 2:972561/1‑139 (MQ=255) cTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatg < 1:1189192/115‑1 (MQ=255) cTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatg > 2:1189192/1‑115 (MQ=255) tgtgTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGa > 1:208172/1‑90 (MQ=255) tgtgTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGa < 2:208172/90‑1 (MQ=255) | GCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAT > NC_000913/1287690‑1287957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |