Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,414,019	C→T	G259R (GGG→AGG)	recT ←	Rac prophage, recombination and repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,414,019	0	C	T	100.0%	173.4 / NA	50	G259R (GGG→AGG)	recT	Rac prophage, recombination and repair protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/28);  total (22/28)

GGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTTGAACAGGCGACGAATAGCCGTTTTCTTTGC  >  NC_000913/1413884‑1414145                                                                                                                                        |                                                                                                                               ggACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGt                                                                                                                             <  1:999394/139‑1 (MQ=255)      aCCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCCGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATa                                                                                                                        >  1:379979/1‑139 (MQ=255)         acaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACag                                                                                                                     >  2:1199120/1‑139 (MQ=255)           aaaaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACagag                                                                                                                   >  2:1105428/1‑139 (MQ=255)             aaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGa                                                                                                                 <  2:100553/139‑1 (MQ=255)             aaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGa                                                                                                                 <  1:868743/139‑1 (MQ=255)                  aCATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTg                                                                                                            >  1:1189204/1‑139 (MQ=255)                   cATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGc                                                                                                           <  2:642368/139‑1 (MQ=255)                       gTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGa                                                                                                       <  2:142777/139‑1 (MQ=255)                           aaCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGa                                                                                                                 >  1:250778/1‑125 (MQ=255)                           aaCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGa                                                                                                                 <  2:250778/125‑1 (MQ=255)                                tGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGCATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGCTTGTc                                                                                              >  1:770820/1‑139 (MQ=255)                                tGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTc                                                                                              >  1:261367/1‑139 (MQ=255)                                tGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTc                                                                                              >  1:1049771/1‑139 (MQ=255)                                 gTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGa                                                                                                                 <  2:63358/119‑1 (MQ=255)                                 gTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGa                                                                                                                 >  1:63358/1‑119 (MQ=255)                                 gTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCa                                                                                             >  1:399703/1‑139 (MQ=255)                                 gTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCa                                                                                             >  1:75060/1‑139 (MQ=255)                                  tCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGa                                                                                                                 >  2:1070865/1‑118 (MQ=255)                                  tCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGa                                                                                                                 <  1:1070865/118‑1 (MQ=255)                                   cATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGt                                                                                           <  2:261367/139‑1 (MQ=255)                                      atCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGt                                                                                        <  2:770820/139‑1 (MQ=255)                                            tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCttt                                                                                  >  2:87231/1‑139 (MQ=255)                                            tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCttt                                                                                  <  1:1105428/139‑1 (MQ=255)                                             aaTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGt                                                                                                                             <  2:973015/95‑1 (MQ=255)                                             aaTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGt                                                                                                                             >  1:973015/1‑95 (MQ=255)                                             aaTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCtttt                                                                                 <  1:444145/139‑1 (MQ=255)                                              aTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTc                                                                                <  1:587555/139‑1 (MQ=255)                                               tttttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCa                                                                               <  2:1189204/139‑1 (MQ=255)                                                    ctcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCAt                                                                          <  2:209924/139‑1 (MQ=255)                                                                 tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGc                                                             <  2:75060/139‑1 (MQ=255)                                                                      aGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGAt                                                        <  1:1199120/139‑1 (MQ=255)                                                                               tGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGa                                               >  2:271921/1‑139 (MQ=255)                                                                                gCATTACACCGCCAGGCTTAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCAGCCATTGATACTGCACGCTGGATCTCAATTGAt                                              <  2:486193/139‑1 (MQ=255)                                                                                gCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGAt                                              <  2:1049771/139‑1 (MQ=255)                                                                                     acacCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACggg                                         >  1:1192598/1‑139 (MQ=255)                                                                                       acCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCa                                       <  2:399703/139‑1 (MQ=255)                                                                                         cGCCAGGCTGAATTATTCCTCAGAATTATCGATAACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCa                                       >  2:1110168/1‑137 (MQ=255)                                                                                         cGCCAGGCTGAATTATTCCTCAGAATTATCGATAACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCa                                       <  1:1110168/137‑1 (MQ=255)                                                                                                 tGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTTGaa                             <  2:1192598/139‑1 (MQ=255)                                                                                                   aaTTATTCCTCTGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCATTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAAGATTTGAACa                           >  2:731241/1‑139 (MQ=255)                                                                                                            tctGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTTGAACAGGCGACGaa                  <  1:271921/139‑1 (MQ=255)                                                                                                             ctGAATTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGCTTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTTGAACAGGCGACGAAt                 >  2:892312/1‑139 (MQ=255)                                                                                                                aaTTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTc                                                                                <  2:1168003/73‑1 (MQ=255)                                                                                                                aaTTATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTc                                                                                >  1:1168003/1‑73 (MQ=255)                                                                                                                   tATCGATTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTTGAACAGGCGACGAATAGCCGt           <  2:379979/139‑1 (MQ=255)                                                                                                                        aTTACACTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTTGAACAGGCGACGAATAGCCGTTTTCt      >  1:1052449/1‑139 (MQ=255)                                                                                                                            cacTGTATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTTGAACAGGCGACGAATAGCCGTTTTCTTTGc  <  1:87231/139‑1 (MQ=255)                                                                                                                                 tATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGAt                                              <  2:356220/90‑1 (MQ=255)                                                                                                                                 tATTCCCTGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGAt                                              >  1:356220/1‑90 (MQ=255)                                                                                                                                        |                                                                                                                               GGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCCTTTTCATCCATTGATACTGCACGCTGGATCTCAATTGATACGGGCAAATATTTGAACAGGCGACGAATAGCCGTTTTCTTTGC  >  NC_000913/1413884‑1414145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑