Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,766,416	A→G	V757A (GTC→GCC)	ydiJ ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,766,416	0	A	G	100.0%	124.1 / NA	33	V757A (GTC→GCC)	ydiJ	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/15);  total (18/15)

CGTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACTGTGCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGACCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATGAAGAAGTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGA  >  NC_000913/1766285‑1766551                                                                                                                                    |                                                                                                                                        cGTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACTGTGCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGAcag                                                                                                                                  >  1:1095138/1‑139 (MQ=255)  gTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACTGTGCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGAcagc                                                                                                                                 >  2:286881/1‑139 (MQ=255)           ggTAAAGGGGTCCTGCACCACCAACACTGTGCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACagcagc                                                                                                                              <  2:383752/133‑1 (MQ=255)           ggTAAAGGGGTCCTGCACCACCAACACTGTGCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACagcagc                                                                                                                              >  1:383752/1‑133 (MQ=255)           ggTAAAGGGGTCCTGCACCACCAACACTGTGCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCaaa                                                                                                                        >  1:291064/1‑139 (MQ=255)           ggTAAAGGGGTCCTGCACCACCAACACTGTGCGCGCTTTCTGCTCTGCATTGAGAAATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGTCGACAGCAGCGGCaaa                                                                                                                        >  2:577268/1‑139 (MQ=255)                 ggggTCCTGCACCACCAACACTGTGCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAAcc                                                                                                                  <  1:466949/139‑1 (MQ=255)                                    acTGTGCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCg                                                                                               <  2:1095138/139‑1 (MQ=255)                                      tgtgCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCgag                                                                                             >  1:1002801/1‑139 (MQ=255)                                       gtgCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAAcc                                                                                                                  >  2:379303/1‑117 (MQ=255)                                       gtgCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAAcc                                                                                                                  <  1:379303/117‑1 (MQ=255)                                         gcgcgcTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGt                                                                                          <  2:291064/139‑1 (MQ=255)                                          cgcgcTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGTGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGtt                                                                                         >  1:259421/1‑139 (MQ=255)                                          cgcgcTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGtt                                                                                         >  1:759699/1‑139 (MQ=255)                                                ttCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCa                                                                                   <  1:286881/139‑1 (MQ=255)                                                            ttGAGAAATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGTCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGt                                                                       <  1:577268/139‑1 (MQ=255)                                                             tGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCg                                                                                                                             <  1:536265/84‑1 (MQ=255)                                                             tGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCg                                                                                                                             >  2:536265/1‑84 (MQ=255)                                                              gagagaTTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTa                                                                     <  2:343125/139‑1 (MQ=255)                                                                             ttCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATGAAGAAGTTAAAg                                                      >  1:626261/1‑139 (MQ=255)                                                                               ccAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATGAAGAAGTTAAAGGt                                                    >  1:1140864/1‑139 (MQ=255)                                                                                                      tGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTgc                                                                                      <  1:634546/82‑1 (MQ=255)                                                                                                      tGCCCCACCATTTGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTgc                                                                                      >  2:634546/1‑82 (MQ=255)                                                                                                                tttGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATgaa                                                                >  1:42725/1‑94 (MQ=255)                                                                                                                tttGTTGTTGTAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATgaa                                                                <  2:42725/94‑1 (MQ=255)                                                                                                                tttGTTGTTGTAGCGAGGGGGCCGACAGCAGC‑GCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCg                                                                             >  2:37836/1‑80 (MQ=255)                                                                                                                tttGTTGTTGTAGCGAGGGGGCCGACAGCAGC‑GCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCg                                                                             <  1:37836/80‑1 (MQ=255)                                                                                                                   gttgttgtAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATGAAGAAGTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGCGCGTAGCtctc                <  2:626261/139‑1 (MQ=255)                                                                                                                       ttgtAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATgaa                                                                >  1:631849/1‑87 (MQ=255)                                                                                                                       ttgtAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATgaa                                                                <  2:631849/87‑1 (MQ=255)                                                                                                                          tAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCgg                                                                            <  1:991094/72‑1 (MQ=255)                                                                                                                          tAGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCgg                                                                            >  2:991094/1‑72 (MQ=255)                                                                                                                           aGCGAGGGGGCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATGAAGAAGTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAg        >  2:257318/1‑139 (MQ=255)                                                                                                                                 ggggCCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATGAAGAAGTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGcgacga  >  1:471267/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        CGTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACTGTGCGCGCTTTCTGCTCTGCATTGAGAGATTCAAGCTGTTCCAGCGTCATGTTTGCCGAGCGATGCCCCACCATTTGTTGTTGTAGCGAGGGGACCGACAGCAGCGGCAAATCAACCATGCCGATATGTTTTTCCGAGAGTTTGCGCACCAGCGGCTGGTTAATGAAGAAGTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGA  >  NC_000913/1766285‑1766551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑