Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,830,359	T→C	N136S (AAC→AGC)	astA ←	arginine succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,830,359	0	T	C	100.0%	89.4 / NA	24	N136S (AAC→AGC)	astA	arginine succinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/14);  total (10/14)

TTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCG  >  NC_000913/1830234‑1830478                                                                                                                              |                                                                                                                        tttGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTgcg                                                                                                            <  1:884374/139‑1 (MQ=255)         aaCGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCg                                                                                                    <  2:20308/139‑1 (MQ=255)                        tgtTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTc                                                                                                      >  1:110908/1‑122 (MQ=255)                        tgtTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTc                                                                                                      <  2:110908/122‑1 (MQ=255)                        tgtTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCCCTTTGCGCCAGTCCGGGTCGAGAAACAGCg                                                                                     >  1:964565/1‑139 (MQ=255)                                    aCCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgc                                                                         <  1:127728/139‑1 (MQ=255)                                            cATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgc                                                                         <  2:508443/131‑1 (MQ=255)                                            cATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgc                                                                         >  1:508443/1‑131 (MQ=255)                                              tttCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGgt                                                               <  1:127524/139‑1 (MQ=255)                                              tttCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGgt                                                               <  1:903388/139‑1 (MQ=255)                                                tCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGgtgt                                                             >  2:35493/1‑139 (MQ=255)                                                   gCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGAt                                                          <  1:35493/139‑1 (MQ=255)                                                          cTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACt                                                   <  1:501768/139‑1 (MQ=255)                                                                    aaaCTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGc                                         >  1:298343/1‑139 (MQ=255)                                                                          gTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc                                   >  2:664829/1‑139 (MQ=255)                                                                           tCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGaa                                                                                           <  1:433174/82‑1 (MQ=255)                                                                           tCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGaa                                                                                           >  2:433174/1‑82 (MQ=255)                                                                             gcgAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAAt                                <  2:1203224/139‑1 (MQ=255)                                                                                 aaaGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGt                            <  1:664829/139‑1 (MQ=255)                                                                                                        cgATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAg     >  2:653001/1‑139 (MQ=255)                                                                                                         gATTTCGACAGCAAATAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAgg    >  2:550500/1‑139 (MQ=255)                                                                                                           tttCGACAGCAAATAGCCGCTGCCCCCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCg  <  2:964565/139‑1 (MQ=255)                                                                                                                     aaaTAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgctgct                                                                     <  1:360657/62‑1 (MQ=255)                                                                                                                     aaaTAGCCGCTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCgctgct                                                                     >  2:360657/1‑62 (MQ=255)                                                                                                                              |                                                                                                                        TTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATTAAACTTGTCGCGAAAAGCCGCCATAAACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGACATTCAGCTCTTTTGAGGCG  >  NC_000913/1830234‑1830478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑