Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,223,735 G→A L517L (CTG→CTA)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,223,7350GA100.0% 166.6 / NA 48L517L (CTG→CTA) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/24);  total (24/24)

TACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGT  >  NC_000913/2223602‑2223861
                                                                                                                                     |                                                                                                                              
tACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTAca                                                                                                                           >  2:873450/1‑139 (MQ=255)
                         ggTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  1:901619/139‑1 (MQ=255)
                         ggTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  1:811060/139‑1 (MQ=255)
                                 aGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGAATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGTGCGCAGTACCCTGCCGAGCATaa                                                                                          <  2:721771/139‑1 (MQ=255)
                                       aTTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  2:93012/125‑1 (MQ=255)
                                       aTTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  >  1:93012/1‑125 (MQ=255)
                                           cGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                <  1:572203/139‑1 (MQ=255)
                                           cGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                <  1:873450/139‑1 (MQ=255)
                                           cGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                <  1:737056/139‑1 (MQ=255)
                                              ccATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGG‑‑CTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGta                                                                           >  1:513834/1‑139 (MQ=255)
                                                  tttATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGtata                                                                         >  1:651882/1‑139 (MQ=255)
                                                           tATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCgg                                                                >  2:121700/1‑139 (MQ=255)
                                                             tGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTAcc                                                                                                       >  1:888141/1‑98 (MQ=255)
                                                             tGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTAcc                                                                                                       <  2:888141/98‑1 (MQ=255)
                                                                   ccATCAGGATTACATAGTGAAAAAAGCCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTg                                                        >  2:643120/1‑139 (MQ=255)
                                                                    cATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGc                                                       <  2:651882/139‑1 (MQ=255)
                                                                    cATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGc                                                       >  1:899342/1‑139 (MQ=255)
                                                                       cAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGa                                                    >  1:1088344/1‑139 (MQ=255)
                                                                         ggATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAg                                                  >  1:50113/1‑139 (MQ=255)
                                                                          gATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGt                                                 >  1:650220/1‑139 (MQ=255)
                                                                               cATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                >  1:1238185/1‑103 (MQ=255)
                                                                               cATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCAt                                                                                <  2:1238185/103‑1 (MQ=255)
                                                                                 tAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATcg                                          <  1:1046252/139‑1 (MQ=255)
                                                                                  aGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATcg                                          >  2:539902/1‑138 (MQ=255)
                                                                                  aGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATcg                                          <  1:539902/138‑1 (MQ=255)
                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  >  1:1052513/1‑80 (MQ=255)
                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  2:1052513/80‑1 (MQ=255)
                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCga                                       >  2:812537/1‑139 (MQ=255)
                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCga                                       >  1:676545/1‑139 (MQ=255)
                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCga                                       >  1:605901/1‑139 (MQ=255)
                                                                                    tGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCga                                       >  1:544961/1‑139 (MQ=255)
                                                                                          aaGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  >  2:240313/1‑74 (MQ=255)
                                                                                          aaGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGcc                                                                                                  <  1:240313/74‑1 (MQ=255)
                                                                                              cGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGt                                                                                   >  1:1008199/1‑85 (MQ=255)
                                                                                              cGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGt                                                                                   <  2:1008199/85‑1 (MQ=255)
                                                                                              cGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCg                             >  2:1144893/1‑139 (MQ=255)
                                                                                               gTGGATGTGCATGCGTTAAAAGAACAGATGCTGG‑‑CTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGAcc                          <  2:513834/139‑1 (MQ=255)
                                                                                                tGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGAc                           >  2:1106753/1‑139 (MQ=255)
                                                                                                 ggATGTGCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGAcc                          <  1:1106753/139‑1 (MQ=255)
                                                                                                       gCATGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGc                    <  2:899342/139‑1 (MQ=255)
                                                                                                         aTGCGTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCAt                  <  2:676545/139‑1 (MQ=255)
                                                                                                             gTTAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAAt              >  2:91643/1‑139 (MQ=255)
                                                                                                               tAAAAGAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATcc            <  2:544961/139‑1 (MQ=255)
                                                                                                                    gAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGa       <  1:91643/139‑1 (MQ=255)
                                                                                                                    gAACAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGa       <  1:643120/139‑1 (MQ=255)
                                                                                                                       cAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGgaga                                                             <  2:1196377/82‑1 (MQ=255)
                                                                                                                       cAGATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTCGTATAAAGCACCGgaga                                                             >  1:1196377/1‑82 (MQ=255)
                                                                                                                         gATGCTGGAACTACTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGt  <  1:812537/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                              
TACCGCCGGAGATCGACAGCCAGCTGGTGCACAAGCTCTATTACGGCCATTTTATGTGCTATGTCTTCCATCAGGATTACATAGTGAAAAAAGGCGTGGATGTGCATGCGTTAAAAGAACAGATGCTGGAACTGCTACAGCAGCGCGGCGCGCAGTACCCTGCCGAGCATAACGTCGGTCATTTGTATAAAGCACCGGAGACGTTGCAGAAGTTCTATCGCGAGAACGATCCGACCAACAGCATGAATCCGGGGATCGGT  >  NC_000913/2223602‑2223861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑