Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,305,466	T→C	E430G (GAG→GGG)	mqo ←	malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,305,466	0	T	C	100.0%	238.1 / NA	63	E430G (GAG→GGG)	mqo	malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/33);  total (30/33)

AAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAG  >  NC_000913/2305333‑2305599                                                                                                                                      |                                                                                                                                      aaaTACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCa                                                                                                                                  <  2:1042704/139‑1 (MQ=255)  aaTACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCAt                                                                                                                                 >  1:37768/1‑139 (MQ=255)   aTACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATc                                                                                                                                <  1:400488/139‑1 (MQ=255)   aTACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATc                                                                                                                                <  2:37768/139‑1 (MQ=255)     aCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCAc                                                                                                                              <  1:798938/139‑1 (MQ=255)          ttCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCGCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCAt                                                                                                                                 <  2:17715/131‑1 (MQ=255)          ttCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCAt                                                                                                                                 >  1:17715/1‑131 (MQ=255)                           tAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTg                                                                                                        <  2:420881/139‑1 (MQ=255)                            aaTCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCTGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGc                                                                                                       >  1:737164/1‑139 (MQ=255)                             aTCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCa                                                                                                                                  >  2:949955/1‑111 (MQ=255)                             aTCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCa                                                                                                                                  <  1:949955/111‑1 (MQ=255)                                 gcgcggcgGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGc                                                                                                  >  1:171177/1‑139 (MQ=255)                                 gcgcggcgGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGc                                                                                                  >  1:38815/1‑139 (MQ=255)                                   gcggcgGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCtt                                                                                                >  2:669186/1‑139 (MQ=255)                                       cggTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCa                                                                                                                               <  2:791575/104‑1 (MQ=255)                                       cggTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCa                                                                                                                               >  1:791575/1‑104 (MQ=255)                                              cGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGc                                                                                     <  2:1217978/139‑1 (MQ=255)                                                           gCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCAc                                                                                                                              >  2:930043/1‑85 (MQ=255)                                                           gCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCAc                                                                                                                              <  1:930043/85‑1 (MQ=255)                                                           gCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCttttt                                                                        >  1:1106160/1‑139 (MQ=255)                                                           gCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCttttt                                                                        >  1:855841/1‑139 (MQ=255)                                                              cccAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCg                                                                     <  2:855841/139‑1 (MQ=255)                                                                cAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGcc                                                                   >  1:876029/1‑139 (MQ=255)                                                                   gAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGc                                                                <  2:1106160/139‑1 (MQ=255)                                                                      cgcgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATc                                                                                                                                <  2:1062341/72‑1 (MQ=255)                                                                      cgcgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATc                                                                                                                                >  1:1062341/1‑72 (MQ=255)                                                                      cgcgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGa                                                             <  2:783897/139‑1 (MQ=255)                                                                      cgcgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGa                                                             <  2:876029/139‑1 (MQ=255)                                                                      cgcgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGa                                                             <  2:171177/139‑1 (MQ=255)                                                                      cgcgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGa                                                             >  2:198117/1‑139 (MQ=255)                                                                      cgcgGCAATGGTTCCTTGCTGGTCAATGACGACTTCAGTACCCAGACGCAGTACGCCACGTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGa                                                             >  2:1130116/1‑139 (MQ=255)                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACg                                                                                                                             <  1:307055/73‑1 (MQ=255)                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACg                                                                                                                             >  2:307055/1‑73 (MQ=255)                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCt                                                                  <  1:85317/132‑1 (MQ=255)                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCt                                                                  >  2:85317/1‑132 (MQ=255)                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATa                                                           >  2:357325/1‑139 (MQ=255)                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATa                                                           >  2:1240319/1‑139 (MQ=255)                                                                         ggCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAg                                                          >  2:627753/1‑139 (MQ=255)                                                                         ggCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAg                                                          <  2:990793/139‑1 (MQ=255)                                                                             aTGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTAct                                                      >  1:1070348/1‑139 (MQ=255)                                                                                   ccTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTATTTTCGCCTGCGGATAGTACTCTTTCa                                                <  2:737164/139‑1 (MQ=255)                                                                                     ttGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGAt                                                            <  2:292242/125‑1 (MQ=255)                                                                                     ttGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGAt                                                            >  1:292242/1‑125 (MQ=255)                                                                                           gTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCt                                                                  <  1:305767/113‑1 (MQ=255)                                                                                           gTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCt                                                                  >  2:305767/1‑113 (MQ=255)                                                                                           gTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCa                                        >  1:86993/1‑139 (MQ=255)                                                                                                  acgacTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGAt                                 >  2:361012/1‑139 (MQ=255)                                                                                                   cgacTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCATGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATc                                <  1:737563/139‑1 (MQ=255)                                                                                                     acTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCct                              <  2:1070348/139‑1 (MQ=255)                                                                                                       ttCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCctct                            <  1:357325/139‑1 (MQ=255)                                                                                                       ttCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCctct                            <  2:738271/139‑1 (MQ=255)                                                                                                                cAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAAc                   >  1:701796/1‑139 (MQ=255)                                                                                                                 aGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACa                  <  2:701796/139‑1 (MQ=255)                                                                                                                  gACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACAt                 >  2:472692/1‑139 (MQ=255)                                                                                                                       aGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCt            <  1:472692/139‑1 (MQ=255)                                                                                                                         tACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGa          <  1:669186/139‑1 (MQ=255)                                                                                                                         tACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGa          >  2:1086892/1‑139 (MQ=255)                                                                                                                             ccACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCa      <  1:627753/139‑1 (MQ=255)                                                                                                                             ccACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCa      <  1:1086892/139‑1 (MQ=255)                                                                                                                                ccTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCa   <  2:859142/139‑1 (MQ=255)                                                                                                                                 cTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAg  >  2:481615/1‑139 (MQ=255)                                                                                                                                   ttCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACt                       >  2:576219/1‑116 (MQ=255)                                                                                                                                   ttCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACt                       <  1:576219/116‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      AAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAG  >  NC_000913/2305333‑2305599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑