| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,335,018 | G→A | L543L (CTG→TTG) | yfaA ← | DUF2138 family protein, putative host defense protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,335,018 | 0 | G | A | 100.0% | 89.7 / NA | 27 | L543L (CTG→TTG) | yfaA | DUF2138 family protein, putative host defense protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/13); total (14/13) | |||||||||||
AGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGCAGCAG > NC_000913/2334895‑2335144 | aGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAAcg > 1:425114/1‑139 (MQ=255)aGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAAcg > 2:231870/1‑139 (MQ=255) cGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGgttgt > 2:1113293/1‑139 (MQ=255) ggCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATa > 2:789223/1‑139 (MQ=255) ggCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATa < 1:464333/139‑1 (MQ=255) caacacaacaCAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCg < 2:871391/139‑1 (MQ=255) acacaacacaGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTc < 1:789223/139‑1 (MQ=255) agccagcAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCgg < 2:870584/139‑1 (MQ=255) agccagcAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCgg > 1:493369/1‑139 (MQ=255) ccagcAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCa > 1:317725/1‑139 (MQ=255) cccGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGt < 2:425114/139‑1 (MQ=255) tGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTgcgg < 1:1235104/132‑1 (MQ=255) tGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTgcgg > 2:1235104/1‑132 (MQ=255) gCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACa > 2:639038/1‑62 (MQ=255) gCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACa < 1:639038/62‑1 (MQ=255) ccAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTACCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCAGTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCgaga > 1:827905/1‑139 (MQ=255) gggTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGc < 1:1113293/139‑1 (MQ=255) gTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAg < 2:493369/139‑1 (MQ=255) tAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGa < 2:317725/139‑1 (MQ=255) gCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACt > 1:1068826/1‑139 (MQ=255) gCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACt > 1:427504/1‑139 (MQ=255) cACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTcc > 2:227806/1‑59 (MQ=255) cACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTcc < 1:227806/59‑1 (MQ=255) cACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGt > 2:368349/1‑90 (MQ=255) cACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGt < 1:368349/90‑1 (MQ=255) ggCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGcagcag < 1:489410/132‑1 (MQ=255) ggCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGcagcag > 2:489410/1‑132 (MQ=255) | AGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGCAGCAG > NC_000913/2334895‑2335144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |