| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,426,751 | G→A | A13V (GCC→GTC) | hisJ ← | histidine ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,426,751 | 0 | G | A | 97.7% | 144.0 / ‑4.8 | 43 | A13V (GCC→GTC) | hisJ | histidine ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (19/23); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGATCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATC > NC_000913/2426624‑2426866 | cAGATCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGaccagaaccaga < 1:962699/139‑1 (MQ=255) aTCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCagagag > 2:12449/1‑139 (MQ=255) aTCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCagagag > 2:1176780/1‑139 (MQ=255) aTCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCagagag > 1:215541/1‑139 (MQ=255) tatCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGa < 2:1030865/139‑1 (MQ=255) tCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATa > 2:683784/1‑139 (MQ=255) cGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAg < 2:1184320/139‑1 (MQ=255) aaGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCa < 2:921311/118‑1 (MQ=255) aaGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCa > 1:921311/1‑118 (MQ=255) aGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCAc < 1:1176780/139‑1 (MQ=255) aGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCAc < 2:316535/139‑1 (MQ=255) aGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGttttt < 1:658173/139‑1 (MQ=255) gCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATa < 1:683784/139‑1 (MQ=255) tgtgAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGt > 2:142603/1‑139 (MQ=255) gAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCt < 2:215541/139‑1 (MQ=255) tGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGAc > 2:375858/1‑139 (MQ=255) tGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGAc < 1:506473/139‑1 (MQ=255) tGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGAc < 1:886006/139‑1 (MQ=255) gATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCa > 1:417768/1‑94 (MQ=255) gATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCa < 2:417768/94‑1 (MQ=255) gcgcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTtat < 2:659124/139‑1 (MQ=255) tAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGa < 2:11570/139‑1 (MQ=255) gtcgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCagagag < 1:125238/81‑1 (MQ=255) gtcgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCagagag > 2:125238/1‑81 (MQ=255) ggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTtat > 1:572236/1‑127 (MQ=255) ggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTtat < 2:572236/127‑1 (MQ=255) gtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAc < 2:38520/139‑1 (MQ=255) gTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAg > 1:1108126/1‑113 (MQ=255) gTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAg < 2:1108126/113‑1 (MQ=255) aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAc > 2:1139645/1‑129 (MQ=255) aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAc < 1:1139645/129‑1 (MQ=255) aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTa > 2:596813/1‑139 (MQ=255) aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTa > 2:638011/1‑139 (MQ=255) gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGAt < 1:671398/67‑1 (MQ=255) gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGAt > 2:671398/1‑67 (MQ=255) gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATa < 2:35339/85‑1 (MQ=255) gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATa > 1:35339/1‑85 (MQ=255) gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACtt > 1:127323/1‑139 (MQ=255) ttttGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCg < 1:142603/139‑1 (MQ=255) ttGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTg > 1:1023204/1‑139 (MQ=255) ggAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCa < 2:325818/40‑1 (MQ=255) ggAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGACCa > 1:325818/1‑40 (MQ=255) aaCGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCAt < 1:638011/139‑1 (MQ=255) aCGCCGCAGTTGCGCTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATc < 2:127323/139‑1 (MQ=255) | CAGATCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATC > NC_000913/2426624‑2426866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |