Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,489,477	T→C	K237R (AAG→AGG)	yfdV ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,489,477	0	T	C	100.0%	212.0 / NA	56	K237R (AAG→AGG)	yfdV	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/27);  total (29/27)

TAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGA  >  NC_000913/2489343‑2489611                                                                                                                                       |                                                                                                                                       tAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAg                                                                                                                                    >  2:221320/1‑139 (MQ=255)    aCCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGaa                                                                                                                                 <  1:820567/139‑1 (MQ=255)     ccGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAg                                                                                                                                <  1:1130778/139‑1 (MQ=255)       ggCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGgt                                                                                                                              <  1:441312/139‑1 (MQ=255)                       tCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAg                                                                                                                                >  2:137791/1‑121 (MQ=255)                       tCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAg                                                                                                                                <  1:137791/121‑1 (MQ=255)                          ggAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCTGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCa                                                                                                           <  1:144240/139‑1 (MQ=255)                             gaATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTg                                                                                                        <  2:871761/139‑1 (MQ=255)                               aTGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGaa                                                                                                      >  1:58789/1‑139 (MQ=255)                                      cggTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATaa                                                                                                                       <  1:745529/115‑1 (MQ=255)                                      cggTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATaa                                                                                                                       >  2:745529/1‑115 (MQ=255)                                           aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACTTGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAgg                                                                                                                                   <  2:589816/98‑1 (MQ=255)                                           aTGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTAGCATCAGAATCAGCCTCAgg                                                                                                                                   >  1:589816/1‑98 (MQ=255)                                             gcgcCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAACCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAtgtg                                                                                        >  1:1032557/1‑139 (MQ=255)                                                ccTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAg                                                                                     >  1:399318/1‑139 (MQ=255)                                                ccTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAg                                                                                     >  1:618402/1‑139 (MQ=255)                                                        tACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTc                                                                             >  2:918465/1‑139 (MQ=255)                                                                  tGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAg                                                                   <  2:345195/139‑1 (MQ=255)                                                                    cAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCg                                                                 >  2:1796/1‑139 (MQ=255)                                                                         gTTCGCTGTTCAAATGACATGCCATACCACCGAGAAGCAATGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTAAACCCCGCAGCGAATAc                                                            >  2:51259/1‑139 (MQ=255)                                                                         gTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAAccc                                                                        >  1:128388/1‑127 (MQ=255)                                                                         gTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAAccc                                                                        <  2:128388/127‑1 (MQ=255)                                                                           tCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCTAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCg                                                          <  1:754002/139‑1 (MQ=255)                                                                            cGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAtgtg                                                                                        >  1:1131288/1‑108 (MQ=255)                                                                            cGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAtgtg                                                                                        <  2:1131288/108‑1 (MQ=255)                                                                            cGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCg                                                          <  1:109505/138‑1 (MQ=255)                                                                            cGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCg                                                          >  2:109505/1‑138 (MQ=255)                                                                                 ttCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTAccc                                                    <  2:271985/139‑1 (MQ=255)                                                                                             gCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCt                                        >  1:675638/1‑139 (MQ=255)                                                                                              ccATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCtt                                       <  1:1796/139‑1 (MQ=255)                                                                                                  aCCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAtgtg                                                                                        >  1:1239879/1‑86 (MQ=255)                                                                                                  aCCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTAtgtg                                                                                        <  2:1239879/86‑1 (MQ=255)                                                                                                  aCCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCa                                   >  1:1087754/1‑139 (MQ=255)                                                                                                  aCCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCa                                   >  1:507497/1‑139 (MQ=255)                                                                                                    cAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAAt                                 <  2:399318/139‑1 (MQ=255)                                                                                                    cAACGAGAAGCAGTGCCAGTGGCATCAGAACCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAAt                                 <  2:1032557/139‑1 (MQ=255)                                                                                                      aCGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCg                                                          >  1:55749/1‑112 (MQ=255)                                                                                                      aCGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCg                                                          <  2:55749/112‑1 (MQ=255)                                                                                                       cGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGaa                                                                                                                                 >  2:266853/1‑40 (MQ=255)                                                                                                       cGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGaa                                                                                                                                 <  1:266853/40‑1 (MQ=255)                                                                                                       cGAGAAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGTGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAg                              >  1:776112/1‑139 (MQ=255)                                                                                                         agaAGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGAt                            >  1:931323/1‑139 (MQ=255)                                                                                                           aaGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCa                                                                                                           >  1:905017/1‑58 (MQ=255)                                                                                                           aaGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCa                                                                                                           <  2:905017/58‑1 (MQ=255)                                                                                                           aaGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTa                          >  1:528356/1‑139 (MQ=255)                                                                                                            aGCAGTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTaa                         <  2:931323/139‑1 (MQ=255)                                                                                                                gTGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGt                     >  1:50253/1‑139 (MQ=255)                                                                                                                 tGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGcc                                                                                   >  1:481528/1‑76 (MQ=255)                                                                                                                 tGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGcc                                                                                   <  2:481528/76‑1 (MQ=255)                                                                                                                 tGCCAGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGtt                    <  2:58789/139‑1 (MQ=255)                                                                                                                     aGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTAccc                                                    >  1:377542/1‑103 (MQ=255)                                                                                                                     aGTGGCATCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTAccc                                                    <  2:377542/103‑1 (MQ=255)                                                                                                                           aTCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCg                                                                      <  2:496619/79‑1 (MQ=255)                                                                                                                           aTCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCg                                                                      >  1:496619/1‑79 (MQ=255)                                                                                                                           aTCAGAATCAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCAtg          <  2:675638/139‑1 (MQ=255)                                                                                                                                   cAGCCTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGa  >  1:800218/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       TAAACCGGCTGGCAATAATGATTCCGGAGAATGCCGGCGGTAATGCGCCTGCCAGTACCATCATCTGCAGATGTTCGCTGTTCAAATGACATGCCATACCAACGAGAAGCAGTGCCAGTGGCATCAGAATCAGCTTCAGGAAGGTGTTATAAGCAATTTCGGCACTGAACTCGAATTTATGTGCAGCCAGAGTCAACCCCGCAGCGAATACCGCTACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGA  >  NC_000913/2489343‑2489611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑