Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,691,927	G→A	A1206V (GCG→GTG)	purL ←	phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,691,927	0	G	A	100.0%	203.8 / NA	58	A1206V (GCG→GTG)	purL	phosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/32);  total (26/32)

CAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGG  >  NC_000913/2691796‑2692059                                                                                                                                    |                                                                                                                                     cAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACg                                                                                                                               <  2:208418/139‑1 (MQ=255)    tCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCAc                                                                                                                            >  1:306322/1‑139 (MQ=255)       tGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGc                                                                                                                              >  2:481466/1‑134 (MQ=255)       tGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGc                                                                                                                              <  1:481466/134‑1 (MQ=255)           tGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcg                                                                                                                     <  2:1127671/139‑1 (MQ=255)           tGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcg                                                                                                                     <  1:872683/139‑1 (MQ=255)                tAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGccc                                                                                                                <  1:917943/139‑1 (MQ=255)                       gTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGc                                                                                                                              <  1:757596/118‑1 (MQ=255)                       gTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGc                                                                                                                              >  2:757596/1‑118 (MQ=255)                       gTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCa                                                                                                         >  1:780957/1‑139 (MQ=255)                        ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCAt                                                                                                        >  2:620897/1‑139 (MQ=255)                         tCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcgcg                                                                                                                   >  2:181835/1‑127 (MQ=255)                         tCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcgcg                                                                                                                   <  1:181835/127‑1 (MQ=255)                         tCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATg                                                                                                       <  2:363475/139‑1 (MQ=255)                                   gTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCa                                                                                             <  1:620897/139‑1 (MQ=255)                                    ttCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCaa                                                                                            >  2:1162580/1‑139 (MQ=255)                                              ccGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTg                                                                                  >  1:575906/1‑139 (MQ=255)                                                  gTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAACAATCGGCATTTGCGAg                                                                              <  2:795551/139‑1 (MQ=255)                                                        ttCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCccacc                                                                        >  1:596538/1‑139 (MQ=255)                                                        ttCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCccacc                                                                        <  2:780957/139‑1 (MQ=255)                                                            gTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATcc                                                                    >  1:1097454/1‑139 (MQ=255)                                                                 tttGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGc                                                                             >  2:327444/1‑125 (MQ=255)                                                                 tttGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGc                                                                             <  1:327444/125‑1 (MQ=255)                                                                 tttGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGc                                                               <  2:1097454/139‑1 (MQ=255)                                                                             aTCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               >  1:837379/1‑111 (MQ=255)                                                                             aTCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               <  2:837379/111‑1 (MQ=255)                                                                              tCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACg                                                  >  1:543765/1‑139 (MQ=255)                                                                                     aGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               >  2:1232694/1‑103 (MQ=255)                                                                                     aGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               <  1:1232694/103‑1 (MQ=255)                                                                                     aGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTg                                           >  1:649141/1‑139 (MQ=255)                                                                                      gcgcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTgg                                          <  2:543765/139‑1 (MQ=255)                                                                                      gcgcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTgg                                          <  2:142906/139‑1 (MQ=255)                                                                                       cgcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCtt                                                                                                              >  2:479860/1‑70 (MQ=255)                                                                                       cgcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCtt                                                                                                              <  1:479860/70‑1 (MQ=255)                                                                                        gcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGt                                        <  2:936798/139‑1 (MQ=255)                                                                                            tGCCACCAGCCCTTTGCTTTCCAGTGCGGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACt                                    >  1:801042/1‑139 (MQ=255)                                                                                            tGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACt                                    >  2:947404/1‑139 (MQ=255)                                                                                            tGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACt                                    >  1:93872/1‑139 (MQ=255)                                                                                             gCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATcc                                                                    <  1:544500/106‑1 (MQ=255)                                                                                             gCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATcc                                                                    >  2:544500/1‑106 (MQ=255)                                                                                              ccaccaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               <  2:1132222/94‑1 (MQ=255)                                                                                              ccaccaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               >  1:1132222/1‑94 (MQ=255)                                                                                                accaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCaa                                <  1:831353/139‑1 (MQ=255)                                                                                                 ccaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAAc                               <  2:649141/139‑1 (MQ=255)                                                                                                 ccaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAAc                               >  1:361114/1‑139 (MQ=255)                                                                                                      ccTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGc                          >  1:894496/1‑139 (MQ=255)                                                                                                             tttCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAAcg                   <  2:393408/139‑1 (MQ=255)                                                                                                               tCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               <  1:623723/77‑1 (MQ=255)                                                                                                               tCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               >  2:623723/1‑77 (MQ=255)                                                                                                                    tGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAg                                                                                               <  2:941359/56‑1 (MQ=255)                                                                                                                    tGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAg                                                                                               >  1:941359/1‑56 (MQ=255)                                                                                                                    tGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCa            <  1:1162580/139‑1 (MQ=255)                                                                                                                      ccgccAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCaaa          <  2:681760/139‑1 (MQ=255)                                                                                                                       cgccAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAg         <  1:82930/139‑1 (MQ=255)                                                                                                                         ccAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCg       <  2:596538/139‑1 (MQ=255)                                                                                                                           aGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGAt     <  1:947404/139‑1 (MQ=255)                                                                                                                           aGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGAt     <  2:361114/139‑1 (MQ=255)                                                                                                                              tGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCgg  >  1:1113080/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                     CAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGG  >  NC_000913/2691796‑2692059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑