| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,724,058 | C→T | D444D (GAC→GAT) | pssA → | phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,724,058 | 0 | C | T | 97.2% | 115.8 / NA | 36 | D444D (GAC→GAT) | pssA | phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (18/17); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAG > NC_000913/2723923‑2724192 | acaGCGAGAAAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCAtcgatcga < 1:794697/139‑1 (MQ=255) caGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATcgatcgat > 2:837730/1‑139 (MQ=255) gATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATca > 1:1092356/1‑139 (MQ=255) aTCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAGGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATGGATTAATTAGCCGCATCCTGTAATcac > 2:713822/1‑139 (MQ=255) cATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTc < 1:619983/139‑1 (MQ=255) aTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACggg > 2:748667/1‑139 (MQ=255) aaGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTc > 1:518047/1‑124 (MQ=255) aaGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTc < 2:518047/124‑1 (MQ=255) cACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATca < 2:942883/111‑1 (MQ=255) cACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATca > 1:942883/1‑111 (MQ=255) tATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTg > 1:143797/1‑139 (MQ=255) tCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGattaat < 2:1119236/87‑1 (MQ=255) tCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGattaat > 1:1119236/1‑87 (MQ=255) tGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGt > 1:775380/1‑139 (MQ=255) aTTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTa < 2:143797/139‑1 (MQ=255) tGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTAtt > 2:360541/1‑139 (MQ=255) ccGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCt > 1:225661/1‑139 (MQ=255) ttATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCttttt < 2:775380/139‑1 (MQ=255) cGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGt > 1:847822/1‑139 (MQ=255) cGTAAACTCATCCGCCGTTTGCGCCGTATACGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGCTTGTTTTTTGGAGGCCACGTGTTGCGTATTCTTTTTGTCTGTTCGCTATTGt < 1:837730/139‑1 (MQ=255) cTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTgg > 2:568914/1‑92 (MQ=255) cTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTgg < 1:568914/92‑1 (MQ=255) cTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCg < 2:951924/126‑1 (MQ=255) cTCATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCg > 1:951924/1‑126 (MQ=255) cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCa < 2:198335/47‑1 (MQ=255) cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCa > 1:198335/1‑47 (MQ=255) cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTa > 2:1081433/1‑107 (MQ=255) cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTa < 1:1081433/107‑1 (MQ=255) cATCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCt > 2:630793/1‑139 (MQ=255) aTCCGCCGTTTGCGCAGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGGGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTg > 2:1093413/1‑139 (MQ=255) tCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAAcccc < 2:117546/63‑1 (MQ=255) tCCGCCGTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAAcccc > 1:117546/1‑63 (MQ=255) ccgccgTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGTGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGa < 2:844613/139‑1 (MQ=255) ccgccgTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGa < 1:748667/139‑1 (MQ=255) cgTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCa < 2:1092356/139‑1 (MQ=255) cgTTTGCGCCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCa < 1:360541/139‑1 (MQ=255) gcgcCGTATCCGCATCGATCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCat < 1:881609/139‑1 (MQ=255) aTCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCAGCCTGTACGGGGTTTGTTGTTTGGAGGCCACGTTTTGCGTATTCTCTATGTCTGTTCGCTATTGTTACTTTCTGGCAGCAACAATATGGCTa > 2:396492/1‑139 (MQ=255) tcgatcgaTTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAg > 2:370990/1‑139 (MQ=255) | ACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAG > NC_000913/2723923‑2724192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |