Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,073,237 T→C N152S (AAC→AGC)  epd ← D‑erythrose 4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,073,2370TC100.0% 282.4 / NA 73N152S (AAC→AGC) epdD‑erythrose 4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/31);  total (42/31)

GATGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCATGCT  >  NC_000913/3073106‑3073372
                                                                                                                                   |                                                                                                                                       
gATGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAAc                                                                                                                                  >  2:1003175/1‑139 (MQ=255)
 aTGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACg                                                                                                                                 <  2:273397/139‑1 (MQ=255)
     tATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGc                                                                                                                             >  1:714822/1‑139 (MQ=255)
     tATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGc                                                                                                                             >  2:707553/1‑139 (MQ=255)
     tATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGc                                                                                                                             >  2:327742/1‑139 (MQ=255)
                aaCCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCa                                                                                                                  <  2:354419/139‑1 (MQ=255)
                 aCCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCAc                                                                                                                 <  1:327742/139‑1 (MQ=255)
                     gTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAg                                                                                                             <  1:434198/139‑1 (MQ=255)
                      ttGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGaaa                                                                                                                                   >  1:170865/1‑116 (MQ=255)
                      ttGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGaaa                                                                                                                                   <  2:170865/116‑1 (MQ=255)
                      ttGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGTGGTGTTCCGCACGAAGt                                                                                                            >  2:1100213/1‑139 (MQ=255)
                         aTCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGa                                                                                                         >  1:43621/1‑139 (MQ=255)
                            gTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATcc                                                                                                      >  2:701471/1‑139 (MQ=255)
                             tGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTGAATACGGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAGGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCt                                                                                                     >  1:829074/1‑139 (MQ=255)
                             tGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCt                                                                                                     >  2:903598/1‑139 (MQ=255)
                             tGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCt                                                                                                     >  2:1029975/1‑139 (MQ=255)
                             tGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATAACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCt                                                                                                     >  1:770721/1‑139 (MQ=255)
                                   gCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCAc                                                                                                                 <  2:138195/121‑1 (MQ=255)
                                   gCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCAc                                                                                                                 >  1:138195/1‑121 (MQ=255)
                                   gCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGa                                                                                               >  2:781334/1‑139 (MQ=255)
                                     ggAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACg                                                                                             <  1:174785/139‑1 (MQ=255)
                                        gTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCg                                                                                          <  1:280153/139‑1 (MQ=255)
                                        gTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCg                                                                                          >  2:1046062/1‑139 (MQ=255)
                                         tGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCg                                                                                                                            <  2:492565/104‑1 (MQ=255)
                                         tGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCg                                                                                                                            >  1:492565/1‑104 (MQ=255)
                                              tgtgGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAaaca                                                                                    >  2:191148/1‑139 (MQ=255)
                                                tgGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAaacaac                                                                                  >  1:358820/1‑139 (MQ=255)
                                                  gTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACgg                                                                                <  2:404885/139‑1 (MQ=255)
                                                  gTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACgg                                                                                >  1:812651/1‑139 (MQ=255)
                                                   tCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGt                                                                               <  1:1029975/139‑1 (MQ=255)
                                                     acaGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCa                                                                                                                  >  2:258164/1‑102 (MQ=255)
                                                     acaGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCa                                                                                                                  <  1:258164/102‑1 (MQ=255)
                                                       aGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTcgcg                                                                           >  2:745756/1‑139 (MQ=255)
                                                         tGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTc                                                                         <  1:1003175/139‑1 (MQ=255)
                                                         tGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTc                                                                         <  2:43621/139‑1 (MQ=255)
                                                              gACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCg                                                                                          >  1:159765/1‑117 (MQ=255)
                                                              gACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCg                                                                                          <  2:159765/117‑1 (MQ=255)
                                                                 tCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGTGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGt                                                                 <  1:701471/139‑1 (MQ=255)
                                                                 tCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGt                                                                 <  2:358820/139‑1 (MQ=255)
                                                                 tCAATACCGTACGCATCATCTAACAATTTGATGACAGGAATTATGCAATTCGTGGTACAGGAATCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGt                                                                 <  1:745756/139‑1 (MQ=255)
                                                                   aaTACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTa                                                               <  2:112756/139‑1 (MQ=255)
                                                                     tACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACt                                                             >  1:719085/1‑139 (MQ=255)
                                                                     tACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACt                                                             >  1:1183794/1‑139 (MQ=255)
                                                                        cGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGTGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGcc                                                          <  1:1100213/139‑1 (MQ=255)
                                                                        cGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGcc                                                          <  1:781334/139‑1 (MQ=255)
                                                                            cGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGa                                                      <  2:719085/139‑1 (MQ=255)
                                                                               atcatcTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGt                                                                                                            >  2:1043660/1‑82 (MQ=255)
                                                                               atcatcTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGt                                                                                                            <  1:1043660/82‑1 (MQ=255)
                                                                               atcatcTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTg                                                                                                <  2:1151195/94‑1 (MQ=255)
                                                                               atcatcTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTg                                                                                                >  1:1151195/1‑94 (MQ=255)
                                                                               atcatcTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACg                                                                                             >  1:346194/1‑97 (MQ=255)
                                                                               atcatcTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACg                                                                                             <  2:346194/97‑1 (MQ=255)
                                                                                           tttGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACt                                       >  1:1152182/1‑139 (MQ=255)
                                                                                           tttGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACt                                       >  1:651268/1‑139 (MQ=255)
                                                                                             tgatgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGa                                                                                                               <  2:481093/65‑1 (MQ=255)
                                                                                             tgatgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGa                                                                                                               >  1:481093/1‑65 (MQ=255)
                                                                                             tgatgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCgag                                                                      >  2:223253/1‑106 (MQ=255)
                                                                                             tgatgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCgag                                                                      <  1:223253/106‑1 (MQ=255)
                                                                                             tgatgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAg                                                        <  2:474591/120‑1 (MQ=255)
                                                                                             tgatgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAg                                                        >  1:474591/1‑120 (MQ=255)
                                                                                             tgatgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACttt                                     >  1:542902/1‑139 (MQ=255)
                                                                                               atgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCga                                                                       >  1:761812/1‑103 (MQ=255)
                                                                                               atgaCGGGAATTATGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCga                                                                       <  2:761812/103‑1 (MQ=255)
                                                                                                           aTGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCcggcgg                       >  1:1134745/1‑139 (MQ=255)
                                                                                                            tGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGt                                                                 <  2:428300/96‑1 (MQ=255)
                                                                                                            tGCAATTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGt                                                                 >  1:428300/1‑96 (MQ=255)
                                                                                                                aTTCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAata                  <  2:770721/139‑1 (MQ=255)
                                                                                                                 ttCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAatat                 >  2:905588/1‑139 (MQ=255)
                                                                                                                 ttCGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAatat                 >  1:201093/1‑139 (MQ=255)
                                                                                                                   cGTGGTACAGGAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATgc               >  1:957383/1‑139 (MQ=255)
                                                                                                                             gAAGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCAt     >  2:112790/1‑139 (MQ=255)
                                                                                                                               aGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGCTGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCATGc   >  2:249951/1‑139 (MQ=255)
                                                                                                                               aGCGCTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCATGc   >  1:686348/1‑139 (MQ=255)
                                                                                                                                gcgcTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCAc                                        <  1:1030631/101‑1 (MQ=255)
                                                                                                                                gcgcTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCATGCt  <  2:201093/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
GATGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAGTGAATTGTGGTCACAGTGCCGGACTCAATACCGTACGCATCATCTAACAATTTGATGACGGGAATTATGCAATTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCATGCT  >  NC_000913/3073106‑3073372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑