Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,120,810	G→A	A157V (GCG→GTG)	glcA ←	glycolate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,120,810	0	G	A	100.0%	157.6 / NA	46	A157V (GCG→GTG)	glcA	glycolate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/21);  total (25/21)

CAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCG  >  NC_000913/3120674‑3120944                                                                                                                                         |                                                                                                                                       cAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCccgcc                                                                                                                                      <  2:315855/139‑1 (MQ=255)      aCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGta                                                                                                                                 >  2:1210724/1‑139 (MQ=255)                aCGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTg                                                                                                                       <  2:849500/139‑1 (MQ=255)                      cGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGccc                                                                                                                 >  2:156923/1‑139 (MQ=255)                         cATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAgg                                                                                                              <  2:553357/139‑1 (MQ=255)                            tGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGccc                                                                                                           >  1:1088155/1‑139 (MQ=255)                                   aTGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACcagca                                                                                                    >  2:249183/1‑139 (MQ=255)                                    tgtgGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACcagcag                                                                                                   <  2:168703/139‑1 (MQ=255)                                     gtgGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCagcagc                                                                                                  <  1:817552/139‑1 (MQ=255)                                      tgGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAgcg                                                                                                 <  1:1074259/139‑1 (MQ=255)                                             gATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGt                                                                                          >  1:282114/1‑139 (MQ=255)                                              aTCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGc                                                                                                                   >  2:943650/1‑113 (MQ=255)                                              aTCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGc                                                                                                                   <  1:943650/113‑1 (MQ=255)                                              aTCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTa                                                                                         >  1:974478/1‑139 (MQ=255)                                              aTCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTa                                                                                         >  2:465064/1‑139 (MQ=255)                                              aTCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTa                                                                                         >  1:22406/1‑139 (MQ=255)                                                  aTTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTa                                                                                         >  2:355095/1‑135 (MQ=255)                                                  aTTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTa                                                                                         <  1:355095/135‑1 (MQ=255)                                                                 ccGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCAc                                                                                 >  2:297424/1‑128 (MQ=255)                                                                 ccGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCAc                                                                                 <  1:297424/128‑1 (MQ=255)                                                                     cGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAagcc                                                                  >  1:727426/1‑139 (MQ=255)                                                                          aGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCg                                                             <  2:368972/139‑1 (MQ=255)                                                                          aGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCg                                                             <  2:974478/139‑1 (MQ=255)                                                                                  cACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAgg                                                                                                              <  1:663312/82‑1 (MQ=255)                                                                                  cACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAgg                                                                                                              >  2:663312/1‑82 (MQ=255)                                                                                     gCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGc                                                  <  2:22406/139‑1 (MQ=255)                                                                                         aaCGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACg                                              <  1:156923/139‑1 (MQ=255)                                                                                                 aaaCGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAg                                      >  1:274967/1‑139 (MQ=255)                                                                                                  aaCGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAgg                                     <  2:319376/139‑1 (MQ=255)                                                                                                    cGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGgag                                   <  1:406158/139‑1 (MQ=255)                                                                                                       cACCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGaaa                                <  2:1088155/139‑1 (MQ=255)                                                                                                        aCCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAAc                               >  2:806591/1‑139 (MQ=255)                                                                                                        aCCGGCGCAGTATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAAc                               >  1:319373/1‑139 (MQ=255)                                                                                                                  tATTGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTaa                     >  2:428365/1‑139 (MQ=255)                                                                                                                    ttGGCAATCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAaca                   <  1:806591/139‑1 (MQ=255)                                                                                                                          aTCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACcag                                                                                                      <  2:697971/50‑1 (MQ=255)                                                                                                                          aTCAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACcag                                                                                                      >  1:697971/1‑50 (MQ=255)                                                                                                                            cAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAaca                   <  2:426749/131‑1 (MQ=255)                                                                                                                            cAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAaca                   >  1:426749/1‑131 (MQ=255)                                                                                                                            cAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCaaa           >  1:497844/1‑139 (MQ=255)                                                                                                                            cAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCaaa           >  2:256817/1‑139 (MQ=255)                                                                                                                            cAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCaaa           >  1:200514/1‑139 (MQ=255)                                                                                                                            cAGACACAGCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCaaa           >  1:146439/1‑139 (MQ=255)                                                                                                                                    gCCCCACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtg   <  2:274967/139‑2 (MQ=255)                                                                                                                                     ccccACCGCGTATACCGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAATCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtcg  >  1:752558/1‑139 (MQ=255)                                                                                                                                     ccccACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCg                                                             <  1:413974/80‑1 (MQ=255)                                                                                                                                     ccccACCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCg                                                             >  2:413974/1‑80 (MQ=255)                                                                                                                                         |                                                                                                                                       CAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCG  >  NC_000913/3120674‑3120944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑