Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,736	T→C	V206A (GTT→GCT)	crp →	cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,736	0	T	C	100.0%	219.2 / NA	58	V206A (GTT→GCT)	crp	cAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/31);  total (27/31)

GGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTT  >  NC_000913/3486606‑3486870                                                                                                                                   |                                                                                                                                       ggTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCa                                                                                                                                <  1:595887/139‑1 (MQ=255) ggTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCa                                                                                                                                <  1:499948/139‑1 (MQ=255)   tATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACt                                                                                                                              >  1:451674/1‑139 (MQ=255)   tATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACt                                                                                                                              >  1:307763/1‑139 (MQ=255)               aaTTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGt                                                                                                                  >  2:694928/1‑139 (MQ=255)                      cGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTg                                                                                                           >  1:214867/1‑139 (MQ=255)                         cAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGgcg                                                                                                        <  2:478516/139‑1 (MQ=255)                           ggATATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGGGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      <  2:451674/139‑1 (MQ=255)                           ggAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      <  2:606619/139‑1 (MQ=255)                           ggAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      <  2:423775/139‑1 (MQ=255)                                   gTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTgg                                                                                              >  1:1000206/1‑139 (MQ=255)                                   gTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTgg                                                                                              >  1:625098/1‑139 (MQ=255)                                              ggCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGc                                                                                                                                 <  2:217151/93‑1 (MQ=255)                                              ggCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGc                                                                                                                                 >  1:217151/1‑93 (MQ=255)                                                  gTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTg                                                                               >  1:878839/1‑139 (MQ=255)                                                  gTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTg                                                                               >  1:701556/1‑139 (MQ=255)                                                  gTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTg                                                                               >  2:999887/1‑139 (MQ=255)                                                     ctcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      >  2:1105518/1‑113 (MQ=255)                                                     ctcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGcgcg                                                                                                      <  1:1105518/113‑1 (MQ=255)                                                     ctcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAgcgcg                                                                                       <  1:156492/128‑1 (MQ=255)                                                     ctcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAgcgcg                                                                                       >  2:156492/1‑128 (MQ=255)                                                             aCCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCTGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAt                                                                    <  2:878839/139‑1 (MQ=255)                                                             aCCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAt                                                                    <  1:1167981/139‑1 (MQ=255)                                                                 tGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGt                                                                              <  1:1189445/125‑1 (MQ=255)                                                                 tGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGt                                                                              >  2:1189445/1‑125 (MQ=255)                                                                 tGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTgg                                                                >  2:611656/1‑139 (MQ=255)                                                                  gggACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGgc                                                               <  1:541508/139‑1 (MQ=255)                                                                   ggACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGgcg                                                              <  1:611656/139‑1 (MQ=255)                                                                   ggACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGgcg                                                              <  2:701556/139‑1 (MQ=255)                                                                      cGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAg                                                           <  2:848808/139‑1 (MQ=255)                                                                      cGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAg                                                           <  2:625098/139‑1 (MQ=255)                                                                        cATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGAc                                                         >  1:560653/1‑139 (MQ=255)                                                                           tCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGa                                                      >  1:335489/1‑139 (MQ=255)                                                                                   tGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGc                                                                                                                                 >  2:826665/1‑56 (MQ=255)                                                                                   tGCTGGAAGATCAGAACCTGATCTCCCCACACGGTACAACCATCGTCGCTTACGGc                                                                                                                                 <  1:826665/56‑1 (MQ=255)                                                                                    gCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  1:127756/139‑1 (MQ=255)                                                                                    gCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  2:307763/139‑1 (MQ=255)                                                                                    gCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             >  2:387536/1‑139 (MQ=255)                                                                                     cTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAccc                                            >  1:21405/1‑139 (MQ=255)                                                                                            aTCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             >  1:622310/1‑131 (MQ=255)                                                                                            aTCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  2:622310/131‑1 (MQ=255)                                                                                              cAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCaa                                   >  2:841876/1‑139 (MQ=255)                                                                                                 aaCCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTa                                <  1:694928/139‑1 (MQ=255)                                                                                                  aCCTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTAt                               <  2:560653/139‑1 (MQ=255)                                                                                                   ccTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATg                              <  1:999887/139‑1 (MQ=255)                                                                                                   ccTGATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATg                              <  2:736100/139‑1 (MQ=255)                                                                                                      gATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  2:744894/121‑1 (MQ=255)                                                                                                      gATCTCCGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             >  1:744894/1‑121 (MQ=255)                                                                                                           ccGCACACGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGa                      >  2:1037099/1‑139 (MQ=255)                                                                                                                 acGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACg                >  1:225282/1‑139 (MQ=255)                                                                                                                 acGGTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACg                <  2:214867/139‑1 (MQ=255)                                                                                                                   ggTAAAACCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCt              <  2:225282/139‑1 (MQ=255)                                                                                                                      aaaaCCATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTGCTCCGATGTGCCGCAGACTGATTTATCACCCCGATATCAACTACGCACTTCGACAAACGCTGGt           >  2:773897/1‑139 (MQ=255)                                                                                                                           cATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGgcg                                                                                                        >  1:333812/1‑41 (MQ=255)                                                                                                                           cATCGTCGCTTACGGCACTCGTTAATCCCGTCGGAGTGgcg                                                                                                        <  2:333812/41‑1 (MQ=255)                                                                                                                             tcgtcgCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTg          <  1:526886/133‑1 (MQ=255)                                                                                                                             tcgtcgCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTg          >  2:526886/1‑133 (MQ=255)                                                                                                                             tcgtcgCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTg          >  1:987088/1‑133 (MQ=255)                                                                                                                             tcgtcgCTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTg          <  2:987088/133‑1 (MQ=255)                                                                                                                              cgtcgcTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             <  2:278289/97‑1 (MQ=255)                                                                                                                              cgtcgcTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCAcc                                             >  1:278289/1‑97 (MQ=255)                                                                                                                               gtcgcTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGttt  >  1:200902/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                       GGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTT  >  NC_000913/3486606‑3486870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑