Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,517,023	G→A	A95V (GCC→GTC)	damX ←	cell division protein that binds to the septal ring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,517,023	0	G	A	100.0%	175.6 / NA	51	A95V (GCC→GTC)	damX	cell division protein that binds to the septal ring
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/19);  total (32/19)

CTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGT  >  NC_000913/3516890‑3517158                                                                                                                                      |                                                                                                                                        ctcGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGactgct                                                                                                                                    >  1:688895/1‑139 (MQ=255)    gCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCtttt                                                                                                                                 >  2:990637/1‑139 (MQ=255)       aGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCCTTTTTg                                                                                                                              >  2:693434/1‑139 (MQ=255)         aCGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTgcg                                                                                                                            >  1:699117/1‑139 (MQ=255)         aCGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTgcg                                                                                                                            <  1:308842/139‑1 (MQ=255)                       cTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGgacgac                                                                                                              <  2:1051674/139‑1 (MQ=255)                       cTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGgacgac                                                                                                              >  1:947529/1‑139 (MQ=255)                       cTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGgacgac                                                                                                              >  1:163904/1‑139 (MQ=255)                                gTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTAcgc                                                                                                     >  1:1016296/1‑139 (MQ=255)                                  cGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGTGGACGACGCTCTACgcgc                                                                                                   >  2:484338/1‑139 (MQ=255)                                  cGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACgcgc                                                                                                   <  1:693434/139‑1 (MQ=255)                                      ggggCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGATCTACGCGCTCtt                                                                                               >  2:1187226/1‑139 (MQ=255)                                           ttttAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATcc                                                                                          <  2:699117/139‑1 (MQ=255)                                           ttttAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATcc                                                                                          <  1:1187226/139‑1 (MQ=255)                                                   cAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTc                                                                                  >  1:753604/1‑139 (MQ=255)                                                      aaCCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTc                                                                                  >  1:27817/1‑136 (MQ=255)                                                      aaCCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTc                                                                                  <  2:27817/136‑1 (MQ=255)                                                                    atCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCAGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCaa                                                                 >  2:546583/1‑139 (MQ=255)                                                                            cagTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTgcg                                                                                                                            >  2:118401/1‑72 (MQ=255)                                                                            cagTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTgcg                                                                                                                            <  1:118401/72‑1 (MQ=255)                                                                              gTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATttcttcttc                                                       >  2:1133808/1‑139 (MQ=255)                                                                               tAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCg                                                      >  1:1229667/1‑139 (MQ=255)                                                                               tAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCg                                                      >  1:1003711/1‑139 (MQ=255)                                                                               tAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCg                                                      <  2:947529/139‑1 (MQ=255)                                                                                  aaCCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGTGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGAtt                                                   <  1:484338/139‑1 (MQ=255)                                                                                         aTGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGa                                                                         >  1:375166/1‑110 (MQ=255)                                                                                         aTGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGa                                                                         <  2:375166/110‑1 (MQ=255)                                                                                               aCGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTAcg                                                                                                      >  2:1199409/1‑75 (MQ=255)                                                                                               aCGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTAcg                                                                                                      <  1:1199409/75‑1 (MQ=255)                                                                                               aCGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCAt                                      >  2:268351/1‑139 (MQ=255)                                                                                                            tataCTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTg                         <  1:990637/139‑1 (MQ=255)                                                                                                               aCTGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTgcgc                      >  2:785865/1‑139 (MQ=255)                                                                                                                 tGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGgacga                                                                                                               <  1:290139/48‑1 (MQ=255)                                                                                                                 tGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGgacga                                                                                                               >  2:290139/1‑48 (MQ=255)                                                                                                                 tGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCAc                    >  1:663174/1‑139 (MQ=255)                                                                                                                 tGACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCAc                    >  2:1208636/1‑139 (MQ=255)                                                                                                                  gACGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACg                   >  2:681640/1‑139 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGctct                                                                                                         <  1:908780/52‑1 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGctct                                                                                                         >  2:908780/1‑52 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATcc                                                                                          >  2:342399/1‑67 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATcc                                                                                          <  1:342399/67‑1 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGAt                                                                        >  2:245441/1‑85 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGAt                                                                        <  1:245441/85‑1 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGAtt                                                   >  2:502716/1‑106 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGAtt                                                   <  1:502716/106‑1 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGa                  >  1:978382/1‑139 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGa                  >  2:938293/1‑139 (MQ=255)                                                                                                                   aCGAGAAGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTGTTTGCGCACGa                  >  1:809845/1‑139 (MQ=255)                                                                                                                         aGCGGGTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGa            <  2:753604/139‑1 (MQ=255)                                                                                                                             ggTTTACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCTTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGc        >  1:762541/1‑139 (MQ=255)                                                                                                                               tttACTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGa      >  1:161344/1‑139 (MQ=255)                                                                                                                                   cTGACTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGt  <  1:1208636/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        CTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTCTTCTTCGATTTCCGGTTCCTCATTGCGCTCTTTTTGCGCACGAGTCGGACGGCGATCGT  >  NC_000913/3516890‑3517158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑