Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,110,959	T→C	A183A (GCA→GCG)	tpiA ←	triosephosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,110,959	0	T	C	100.0%	166.8 / NA	45	A183A (GCA→GCG)	tpiA	triosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

GCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTT  >  NC_000913/4110835‑4111089                                                                                                                             |                                                                                                                                   gCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGt                                                                                                                      >  1:499246/1‑139 (MQ=255)     aGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCa                                                                                                                  >  1:1006272/1‑139 (MQ=255)     aGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCa                                                                                                                  >  2:1078529/1‑139 (MQ=255)       cAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGa                                                                                                                <  1:389796/139‑1 (MQ=255)           cAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTg                                                                                                            <  2:1006272/139‑1 (MQ=255)                   cAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTAcc                                                                                                    >  2:299077/1‑139 (MQ=255)                      cGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGAt                                                                                                 <  2:1210864/139‑1 (MQ=255)                       gTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGAtt                                                                                                <  2:616116/139‑1 (MQ=255)                         tAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCACCTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGc                                                                                              <  2:748653/139‑1 (MQ=255)                           gACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGccc                                                                                            >  2:783716/1‑139 (MQ=255)                               cgTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATAc                                                                                        <  1:1078529/139‑1 (MQ=255)                                   tACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGt                                                                                    >  1:24726/1‑139 (MQ=255)                                             ccgTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAgaga                                                                          >  2:901950/1‑139 (MQ=255)                                                       tGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCAcc                                                                >  1:287234/1‑139 (MQ=255)                                                          tCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTc                                                             >  2:270820/1‑139 (MQ=255)                                                          tCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTc                                                             >  2:316253/1‑139 (MQ=255)                                                               tGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGc                                                                            >  1:532917/1‑119 (MQ=255)                                                               tGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGc                                                                            <  2:532917/119‑1 (MQ=255)                                                                 ttCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGcc                                                      >  2:743950/1‑139 (MQ=255)                                                                      cGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCgcagc                                                 >  1:736808/1‑139 (MQ=255)                                                                         tGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCAcc                                              <  2:24726/139‑1 (MQ=255)                                                                          gTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACATCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCAccc                                             <  2:400480/139‑1 (MQ=255)                                                                           ttAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCt                                            >  1:218879/1‑139 (MQ=255)                                                                             aGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGa                                          <  1:197632/139‑1 (MQ=255)                                                                              gCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAg                                         <  1:316253/139‑1 (MQ=255)                                                                              gCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAg                                         <  1:270820/139‑1 (MQ=255)                                                                                     cTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAg                                  >  1:516048/1‑139 (MQ=255)                                                                                               gtgGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCg                        >  1:1024061/1‑139 (MQ=255)                                                                                                tgGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGa                       >  1:130979/1‑139 (MQ=255)                                                                                                tgGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATT‑CCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTa                                >  2:1106155/1‑129 (MQ=255)                                                                                                tgGTCACGGATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATT‑CCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTa                                <  1:1106155/129‑1 (MQ=255)                                                                                                        gATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCg                                                            <  1:924590/94‑1 (MQ=255)                                                                                                        gATGAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCg                                                            >  2:924590/1‑94 (MQ=255)                                                                                                           gAATTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTgcg            <  2:499246/139‑1 (MQ=255)                                                                                                            aaTTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTgcgc           >  1:33178/1‑139 (MQ=255)                                                                                                            aaTTTGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTgcgc           >  1:177455/1‑139 (MQ=255)                                                                                                              tttGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaa         <  2:736808/139‑1 (MQ=255)                                                                                                               ttGTGAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaaa        <  1:299077/139‑1 (MQ=255)                                                                                                                 gtgAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGc                                                                            <  2:954704/69‑1 (MQ=255)                                                                                                                 gtgAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGc                                                                            >  1:954704/1‑69 (MQ=255)                                                                                                                 gtgAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaa         <  2:104311/136‑1 (MQ=255)                                                                                                                 gtgAACAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCaa         >  1:104311/1‑136 (MQ=255)                                                                                                                     aCAGCCTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAActtctt  <  1:955471/139‑1 (MQ=255)                                                                                                                         ccTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCt                                            >  2:288719/1‑93 (MQ=255)                                                                                                                         ccTGCGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCt                                            <  1:288719/93‑1 (MQ=255)                                                                                                                             |                                                                                                                                   GCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGGATGAATTTGTGAACAGCCTGTGCCTGAGCCGGAGTTGCAGATTTGCCAGTACCGATTGCCCATACAGGTTCGTAAGCGATAACCGCACCTTCGAATGCCGCAGCACCCTGAGTTTTCAGTACCGCGTCGATCTGACGTGCGCAAACTTCTT  >  NC_000913/4110835‑4111089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑