Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,231,599	C→T	M11I (ATG→ATA)	yjbD ←	DUF3811 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,231,599	0	C	T	100.0%	196.3 / NA	56	M11I (ATG→ATA)	yjbD	DUF3811 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

ACGCTCAACCATTAATTTATCGATGTAATCTTTCTTAATGCTGTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTCATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGAACTGATAATATTCATTTTGCCTTCTCTTAACTATTTATCTCTTTC  >  NC_000913/4231470‑4231733                                                                                                                                  |                                                                                                                                       aCGCTCAACCATTAAATTATCGATGTAATCTTTCTTAATGCTGTTGGTTTCGGCGCTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCtttt                                                                                                                               >  2:375207/1‑139 (MQ=255)      cAACCATTAATTTATCGATGTAATCTTTCTTAATGCTGTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGt                                                                                                                          >  1:991252/1‑139 (MQ=255)                   aTCGATGTAATCTTTCTTAATGCTGTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGt                                                                                                             >  1:239505/1‑139 (MQ=255)                                  cttAATGCTGTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTggg                                                                                                                           <  1:927365/110‑1 (MQ=255)                                  cttAATGCTGTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTggg                                                                                                                           >  2:927365/1‑110 (MQ=255)                                           gTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTggg                                                                                                                           >  2:146280/1‑101 (MQ=255)                                           gTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTggg                                                                                                                           <  1:146280/101‑1 (MQ=255)                                           gTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTg                                                                                                                             <  1:1042739/99‑1 (MQ=255)                                           gTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTg                                                                                                                             >  2:1042739/1‑99 (MQ=255)                                           gTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACgggg                                                                                                                 >  2:965218/1‑111 (MQ=255)                                           gTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACgggg                                                                                                                 <  1:965218/111‑1 (MQ=255)                                           gTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCa                                                                                           >  2:767166/1‑133 (MQ=255)                                           gTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCa                                                                                           <  1:767166/133‑1 (MQ=255)                                            ttGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTggg                                                                                                                           <  1:224127/100‑1 (MQ=255)                                            ttGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTggg                                                                                                                           >  2:224127/1‑100 (MQ=255)                                            ttGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGcc                                                                                    >  2:692607/1‑139 (MQ=255)                                             tGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATgg                                                                                                   <  1:358058/123‑1 (MQ=255)                                             tGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATgg                                                                                                   >  2:358058/1‑123 (MQ=255)                                                   cGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCa                                                                                           >  2:608379/1‑125 (MQ=255)                                                   cGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCa                                                                                           <  1:608379/125‑1 (MQ=255)                                                   cGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGt                                                                             >  1:513721/1‑139 (MQ=255)                                                      aGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCa                                                                          <  1:692607/139‑1 (MQ=255)                                                            tCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTAc                                                                    <  2:991252/139‑1 (MQ=255)                                                                        cATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGttt                                                        >  1:1208147/1‑139 (MQ=255)                                                                          tGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATa                                                                                                                      >  2:1176265/1‑75 (MQ=255)                                                                          tGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATa                                                                                                                      <  1:1176265/75‑1 (MQ=255)                                                                          cgcgcAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGGTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGc                                                      <  2:1235859/138‑1 (MQ=255)                                                                             gcAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAg                                                   <  2:1208147/139‑1 (MQ=255)                                                                                 tCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGa                                               >  2:988334/1‑139 (MQ=255)                                                                                 tCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGa                                               >  2:180050/1‑139 (MQ=255)                                                                                 tCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGa                                               >  2:862094/1‑139 (MQ=255)                                                                                   cgcgCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGAAc                                             <  1:1078855/139‑1 (MQ=255)                                                                                     cgcCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAg                                                   <  1:522410/131‑1 (MQ=255)                                                                                     cgcCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAg                                                   >  2:522410/1‑131 (MQ=255)                                                                                           aTCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGa                                                                                                               <  2:409370/65‑1 (MQ=255)                                                                                           aTCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGa                                                                                                               >  1:409370/1‑65 (MQ=255)                                                                                           aTCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCt                                                                                             <  1:588402/83‑1 (MQ=255)                                                                                           aTCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCt                                                                                             >  2:588402/1‑83 (MQ=255)                                                                                                      ttttAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGcc                                                                                               <  1:1133825/70‑1 (MQ=255)                                                                                                      ttttAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGcc                                                                                               >  2:1133825/1‑70 (MQ=255)                                                                                                      ttttAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGtt                                                                            >  2:423971/1‑89 (MQ=255)                                                                                                      ttttAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGtt                                                                            <  1:423971/89‑1 (MQ=255)                                                                                                      ttttAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGttt                                                        >  2:379861/1‑109 (MQ=255)                                                                                                      ttttAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGttt                                                        <  1:379861/109‑1 (MQ=255)                                                                                                      ttttAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGa                                               <  2:1194430/118‑1 (MQ=255)                                                                                                      ttttAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGa                                               >  1:1194430/1‑118 (MQ=255)                                                                                                        ttAAGTTCACGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGAACTGATAATATTCATTTTGCCtt                        <  1:375207/139‑1 (MQ=255)                                                                                                                aCGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGAACTGATAATATTCATTTTGCCTTCTCTTaa                 <  2:266518/138‑1 (MQ=255)                                                                                                                aCGCTGCTCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGAACTGATAATATTCATTTTGCCTTCTCTTaa                 >  1:266518/1‑138 (MQ=255)                                                                                                                  gctgctCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCatgat                                                                                                     >  2:376859/1‑52 (MQ=255)                                                                                                                  gctgctCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCatgat                                                                                                     <  1:376859/52‑1 (MQ=255)                                                                                                                     gctCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGAAc                                             >  2:353095/1‑105 (MQ=255)                                                                                                                     gctCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGAAc                                             <  1:353095/105‑1 (MQ=255)                                                                                                                      ctCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAg                                                   <  1:141754/98‑1 (MQ=255)                                                                                                                      ctCGCGTTCAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAg                                                   >  2:141754/1‑98 (MQ=255)                                                                                                                              cAGTTATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGAACTGATAATATTCATTTTGCCTTCTCTTAACTATTTATCTCTTTc  >  2:487273/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                       ACGCTCAACCATTAATTTATCGATGTAATCTTTCTTAATGCTGTTGGTTTCGGAGTTGGTCAATACGCGACCATGCGCAATCCGCGCCCGATCCAGCAGTGTTTTAAGTTCACGCTGCTCGCGTTCAGTCATCTCTTTTTGGGTAATACGGGGGAGTGCCATGATGGTGCCCTCAGTGAGCCAGAGAGTTCAGTGTACGGGAATTCGTTTGCGAGCAGAACTGATAATATTCATTTTGCCTTCTCTTAACTATTTATCTCTTTC  >  NC_000913/4231470‑4231733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑