Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,305,268	A→G	Y444H (TAT→CAT)	yjcS ←	metallo‑beta‑lactamase superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,305,268	0	A	G	100.0%	68.5 / NA	19	Y444H (TAT→CAT)	yjcS	metallo‑beta‑lactamase superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

CCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGC  >  NC_000913/4305142‑4305392                                                                                                                               |                                                                                                                             ccTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGtt                                                                                                                  <  1:801736/139‑1 (MQ=255)     tttCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAgg                                                                                                                    >  2:738863/1‑133 (MQ=255)     tttCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAgg                                                                                                                    <  1:738863/133‑1 (MQ=255)                              tAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGAt                                                                                     <  1:1091182/139‑1 (MQ=255)                                        gcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCggg                                                                                                           <  2:831135/107‑1 (MQ=255)                                        gcttgTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCggg                                                                                                           >  1:831135/1‑107 (MQ=255)                                                   tcttGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCggg                                                                >  1:917602/1‑139 (MQ=255)                                                              gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGTTg                                                     >  2:1161851/1‑139 (MQ=255)                                                              gTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTg                                                     >  2:481407/1‑139 (MQ=255)                                                               ttGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGa                                                    <  2:448083/139‑1 (MQ=255)                                                                  atgaCACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAg                                                 >  1:656114/1‑139 (MQ=255)                                                                  atgaCACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGAAAg                                                 >  2:9670/1‑139 (MQ=255)                                                                         gggCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTa                                          >  1:225922/1‑139 (MQ=255)                                                                                         cgcCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGcc                                                                                          <  2:1138661/75‑1 (MQ=255)                                                                                         cgcCTGCACGTAACGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGcc                                                                                          >  1:1138661/1‑75 (MQ=255)                                                                                                      cGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAg                                                                                   >  2:473678/1‑69 (MQ=255)                                                                                                      cGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAg                                                                                   <  1:473678/69‑1 (MQ=255)                                                                                                      cGTTTACCCATCTCCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCaa             <  1:481407/139‑1 (MQ=255)                                                                                                                 ctcCACCTGACCATGCGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAggcggc  >  1:390979/1‑139 (MQ=255)                                                                                                                               |                                                                                                                             CCTGTTTCAGCAGTTCTGCCGACCAGCGGTAATCACCTTGCTTGTTCGCTTCTTGCGCCAGGTTGATGACACGGGCAGAACCGCCCAGCGCCTGCACGTAACGTTTACCCATCTCCACCTGACCATACGGATGCAGGTTAGCCGGGTTACCGTCGTAATAGCCAAGATAGAAGTTATACACCGCGCGGGCGTTGTGGCTGACAGAACCGTAATAGCCGCGGCTGGCCCAGTTATTGGCAAGTGCAGGCGGC  >  NC_000913/4305142‑4305392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑