Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,491,277 T→C T417A (ACG→GCG)  idnT ← L‑idonate and D‑gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,491,2770TC100.0% 169.5 / NA 44T417A (ACG→GCG) idnTL‑idonate and D‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/19);  total (25/19)

CGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGG  >  NC_000913/4491152‑4491410
                                                                                                                             |                                                                                                                                     
cGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAAc                                                                                                                          >  2:864510/1‑139 (MQ=255)
     ggggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTc                                                                                                                     >  2:229466/1‑139 (MQ=255)
              tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt                                                                                                            <  1:864510/139‑1 (MQ=255)
                       ccccGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa                                                                                                   >  2:1042139/1‑139 (MQ=255)
                        cccGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaaa                                                                                                  <  2:1098574/139‑1 (MQ=255)
                         ccGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt                                                                                                 <  1:229466/139‑1 (MQ=255)
                                       caATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGc                                                                                   >  2:728144/1‑139 (MQ=255)
                                               tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGcc                                                                                  <  1:896081/132‑1 (MQ=255)
                                               tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGcc                                                                                  >  2:896081/1‑132 (MQ=255)
                                                      tCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCg                                                                    >  2:62557/1‑139 (MQ=255)
                                                      tCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCg                                                                    >  1:1073947/1‑139 (MQ=255)
                                                                   gCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGAcg                                                       <  1:1031580/139‑1 (MQ=255)
                                                                    cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCa                                                                                 >  2:191043/1‑112 (MQ=255)
                                                                    cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCa                                                                                 <  1:191043/112‑1 (MQ=255)
                                                                    cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGAcgc                                                      >  2:261870/1‑139 (MQ=255)
                                                                    cGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGAcgc                                                      >  2:629810/1‑139 (MQ=255)
                                                                      ttAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGa                                                    >  2:422743/1‑139 (MQ=255)
                                                                      ttAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGa                                                    >  2:927548/1‑139 (MQ=255)
                                                                       tAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTc                                                                                                           <  1:533525/83‑1 (MQ=255)
                                                                       tAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTc                                                                                                           >  2:533525/1‑83 (MQ=255)
                                                                                acacGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGc                                                                                                                             <  2:639135/56‑1 (MQ=255)
                                                                                acacGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGc                                                                                                                             >  1:639135/1‑56 (MQ=255)
                                                                                  acGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCg                                        <  1:629810/139‑1 (MQ=255)
                                                                                    gCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGcc                                                                           >  1:475441/1‑102 (MQ=255)
                                                                                    gCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGcc                                                                           <  2:475441/102‑1 (MQ=255)
                                                                                     cccAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACgt                                                             <  1:693291/115‑1 (MQ=255)
                                                                                     cccAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACgt                                                             >  2:693291/1‑115 (MQ=255)
                                                                                      ccAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCAcc                                    >  2:676065/1‑139 (MQ=255)
                                                                                                        aaaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt                                                                                                            >  1:566434/1‑49 (MQ=255)
                                                                                                        aaaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt                                                                                                            <  2:566434/49‑1 (MQ=255)
                                                                                                        aaaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt                                                                                                            >  1:235813/1‑49 (MQ=255)
                                                                                                        aaaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt                                                                                                            <  2:235813/49‑1 (MQ=255)
                                                                                                         aaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt                                                                                                 >  1:1179584/1‑59 (MQ=255)
                                                                                                         aaTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt                                                                                                 <  2:1179584/59‑1 (MQ=255)
                                                                                                          aTAAGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAg                <  2:1073947/139‑1 (MQ=255)
                                                                                                             aGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCgg             >  1:404765/1‑139 (MQ=255)
                                                                                                             aGGGTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCgg             >  1:902932/1‑139 (MQ=255)
                                                                                                               ggTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGa           >  2:1114455/1‑139 (MQ=255)
                                                                                                                gTTTCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGAt          <  2:404765/139‑1 (MQ=255)
                                                                                                                   tCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGt                                                                                                            >  2:1106527/1‑38 (MQ=255)
                                                                                                                   tCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGt                          >  2:568540/1‑120 (MQ=255)
                                                                                                                   tCCATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGt                          <  1:568540/120‑1 (MQ=255)
                                                                                                                    ccATCACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGc      <  1:676065/139‑1 (MQ=255)
                                                                                                                        cACCGCCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATgg  <  1:361475/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                     
CGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGG  >  NC_000913/4491152‑4491410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑