Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 742,336 C→T G74D (GGC→GAC)  dtpD ← dipeptide and tripeptide permease D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913742,3360CT86.4% 205.3 / 21.5 81G74D (GGC→GAC) dtpDdipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand):  ref base C (7/4);  new base T (36/34);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAG  >  NC_000913/742201‑742457
                                                                                                                                       |                                                                                                                         
gATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCga                                                                                                                        <  2:462989/139‑1 (MQ=255)
      aaCAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACtt                                                                                                                  >  1:503078/1‑139 (MQ=255)
           gCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATcc                                                                                                             >  1:620597/1‑139 (MQ=255)
                     cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGc                                                                                                   >  1:1024662/1‑139 (MQ=255)
                     cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGc                                                                                                   >  1:1306110/1‑139 (MQ=255)
                      aGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGcc                                                                                                  >  2:821666/1‑139 (MQ=255)
                       gACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCa                                                                                                 >  2:322640/1‑139 (MQ=255)
                              aTCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGa                                                                                          <  2:1024662/139‑1 (MQ=255)
                                   cAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGc                                                                                     <  1:322640/139‑1 (MQ=255)
                                         cAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACa                                                                               <  2:513765/139‑1 (MQ=255)
                                            aTAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATa                                                                             >  2:1426318/1‑138 (MQ=255)
                                            aTAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATa                                                                             <  1:1426318/138‑1 (MQ=255)
                                                   aaTGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCg                                                                     <  1:1457304/139‑1 (MQ=255)
                                                      gACGGATGGATCTCATTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGTGATTGTCGAGAACTTTa                                                                                                                >  2:957707/1‑93 (MQ=255)
                                                      gACGGATGGATCTCATTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGTGATTGTCGAGAACTTTa                                                                                                                <  1:957707/93‑1 (MQ=255)
                                                      gACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAAc                                                                  >  1:1263561/1‑139 (MQ=255)
                                                      gACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAAc                                                                  >  2:528799/1‑139 (MQ=255)
                                                       aCGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACa                                                                 >  2:329550/1‑139 (MQ=255)
                                                         ggatggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGt                                                               <  1:18393/139‑1 (MQ=255)
                                                          gatggatCTGACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATGCACCAGCGAACAGTa                                                              >  2:1104556/1‑139 (MQ=255)
                                                          gatggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTa                                                              >  1:1525113/1‑139 (MQ=255)
                                                            tggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAgg                                                            >  1:1162477/1‑139 (MQ=255)
                                                              gatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGgcg                                                          <  1:329550/139‑1 (MQ=255)
                                                                    aCTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAAt                                                    >  1:125733/1‑139 (MQ=255)
                                                                      tGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATaa                                                  >  2:1034261/1‑139 (MQ=255)
                                                                        gCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACt                                                >  1:891899/1‑139 (MQ=255)
                                                                         cACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGc                                                                                     <  1:403202/101‑1 (MQ=255)
                                                                         cACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGc                                                                                     >  2:403202/1‑101 (MQ=255)
                                                                         cACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTc                                               >  2:321567/1‑139 (MQ=255)
                                                                          aCCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg                                              <  2:400776/139‑1 (MQ=255)
                                                                          aCCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg                                              >  2:630124/1‑139 (MQ=255)
                                                                           cccAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTaaa                                                     <  1:878865/131‑1 (MQ=255)
                                                                           cccAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTaaa                                                     >  2:878865/1‑131 (MQ=255)
                                                                            ccAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTa                                            <  2:1030699/139‑1 (MQ=255)
                                                                            ccAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTa                                            <  2:1263561/139‑1 (MQ=255)
                                                                              aGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAgg                                          >  1:76021/1‑139 (MQ=255)
                                                                                 accacATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGt                                       <  2:1612363/139‑1 (MQ=255)
                                                                                    acaTGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg                                    <  1:1430462/139‑1 (MQ=255)
                                                                                     caTGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAg                                                                                                    >  1:1434744/1‑74 (MQ=255)
                                                                                     caTGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAg                                                                                                    <  2:1434744/74‑1 (MQ=255)
                                                                                       tGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTAt                                 <  2:1304067/139‑1 (MQ=255)
                                                                                          ccGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGt                                                               >  2:434211/1‑106 (MQ=255)
                                                                                          ccGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGt                                                               <  1:434211/106‑1 (MQ=255)
                                                                                             aTCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCAcc                                                                                               <  2:285699/71‑1 (MQ=255)
                                                                                             aTCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCAcc                                                                                               >  1:285699/1‑71 (MQ=255)
                                                                                             aTCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg                                    <  2:1277965/130‑1 (MQ=255)
                                                                                             aTCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg                                    >  1:1277965/1‑130 (MQ=255)
                                                                                             aTCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGt                              >  1:765712/1‑136 (MQ=255)
                                                                                             aTCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGt                              <  2:765712/136‑1 (MQ=255)
                                                                                             aTCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGt                           >  1:51406/1‑139 (MQ=255)
                                                                                             aTCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGt                           >  2:575293/1‑139 (MQ=255)
                                                                                                   aTCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg                                    <  2:689937/124‑1 (MQ=255)
                                                                                                   aTCAACAACGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg                                    >  1:689937/1‑124 (MQ=255)
                                                                                                        caacGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGa                >  2:671498/1‑139 (MQ=255)
                                                                                                         aacGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAt               <  2:51406/139‑1 (MQ=255)
                                                                                                           cGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAAc                                                 <  2:389585/103‑1 (MQ=255)
                                                                                                           cGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAAc                                                 >  1:389585/1‑103 (MQ=255)
                                                                                                           cGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTg             >  1:108338/1‑139 (MQ=255)
                                                                                                           cGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTg             <  2:125733/139‑1 (MQ=255)
                                                                                                           cGCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTg             >  2:594756/1‑139 (MQ=255)
                                                                                                            gCCCCCAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTgg            >  2:727441/1‑139 (MQ=255)
                                                                                                             cccccAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGt           <  1:897167/139‑1 (MQ=255)
                                                                                                             cccccAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGt           <  2:108338/139‑1 (MQ=255)
                                                                                                             cccccAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGt           <  1:630124/139‑1 (MQ=255)
                                                                                                             cccccAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGt           <  1:1034261/139‑1 (MQ=255)
                                                                                                              ccccAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGc                                                                                     <  2:205981/64‑1 (MQ=255)
                                                                                                              ccccAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGc                                                                                     >  1:205981/1‑64 (MQ=255)
                                                                                                               cccAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTga         <  1:55683/139‑1 (MQ=255)
                                                                                                               cccAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTga         <  1:321567/139‑1 (MQ=255)
                                                                                                                ccAGCATCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTgag        >  2:407832/1‑139 (MQ=255)
                                                                                                                      tCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCg                                                                                    >  1:755695/1‑57 (MQ=255)
                                                                                                                      tCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCg                                                                                    <  2:755695/57‑1 (MQ=255)
                                                                                                                      tCACCGCCATGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATagag  >  1:1142146/1‑139 (MQ=255)
                                                                                                                          cGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAt               >  2:179590/1‑122 (MQ=255)
                                                                                                                          cGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAt               <  1:179590/122‑1 (MQ=255)
                                                                                                                              aTGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGgc                                                           <  1:901178/74‑1 (MQ=255)
                                                                                                                              aTGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGgc                                                           >  2:901178/1‑74 (MQ=255)
                                                                                                                              aTGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGt                                       >  1:1204514/1‑94 (MQ=255)
                                                                                                                              aTGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGt                                       <  2:1204514/94‑1 (MQ=255)
                                                                                                                              aTGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTa                                  <  2:591502/99‑1 (MQ=255)
                                                                                                                              aTGCGATTGTCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTa                                  >  1:591502/1‑99 (MQ=255)
                                                                                                                                       |                                                                                                                         
GATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAG  >  NC_000913/742201‑742457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: