Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	924,093	A→G	D277G (GAC→GGC)	clpA →	ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	924,093	0	A	G	100.0%	158.6 / NA	42	D277G (GAC→GGC)	clpA	ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/20);  total (22/20)

TTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAC  >  NC_000913/923958‑924228                                                                                                                                        |                                                                                                                                        ttCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGgcac                                                                                                                                      <  1:653425/139‑1 (MQ=255)                cGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGtt                                                                                                                       <  1:845767/139‑1 (MQ=255)                  gAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTa                                                                                                                     <  1:1054060/139‑1 (MQ=255)                   aaGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTAt                                                                                                                    <  2:265302/139‑1 (MQ=255)                       gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgat                                                                                                                >  1:1204243/1‑139 (MQ=255)                       gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgat                                                                                                                >  2:1092176/1‑139 (MQ=255)                       gATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgat                                                                                                                >  2:820305/1‑139 (MQ=255)                        aTGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgatg                                                                                                               <  2:637198/139‑1 (MQ=255)                        aTGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgatg                                                                                                               <  2:1204243/139‑1 (MQ=255)                                cTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCcac                                                                                                       <  1:1092176/139‑1 (MQ=255)                                           aCTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgat                                                                                                                >  1:1511248/1‑119 (MQ=255)                                           aCTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgat                                                                                                                <  2:1511248/119‑1 (MQ=255)                                                ctcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCgg                                                                                       >  1:125706/1‑139 (MQ=255)                                                  cGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGt                                                                                     >  1:867898/1‑139 (MQ=255)                                                               ctGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCtggtg                                                                        >  2:670200/1‑139 (MQ=255)                                                                 gTTAGCGGTCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCTGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTggtggt                                                                      <  1:1000850/139‑1 (MQ=255)                                                                          cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgat                                                                                                                >  2:1565607/1‑88 (MQ=255)                                                                          cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgat                                                                                                                <  1:1565607/88‑1 (MQ=255)                                                                          cacaAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGAt                                                             >  2:857385/1‑139 (MQ=255)                                                                                       ggcgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTAt                                                                                                                    <  1:1379089/71‑1 (MQ=255)                                                                                       ggcgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTAt                                                                                                                    >  2:1379089/1‑71 (MQ=255)                                                                                       ggcgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTaa                                                >  1:1074379/1‑139 (MQ=255)                                                                                       ggcgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTaa                                                >  1:195737/1‑139 (MQ=255)                                                                                         cgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGgcac                                                                                                                                      <  1:264186/51‑1 (MQ=255)                                                                                         cgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGgcac                                                                                                                                      >  2:264186/1‑51 (MQ=255)                                                                                         cgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACa                                                                                                                                 >  2:1169499/1‑56 (MQ=255)                                                                                         cgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACa                                                                                                                                 <  1:1169499/56‑1 (MQ=255)                                                                                         cgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGAt                                                             >  2:1457420/1‑124 (MQ=255)                                                                                         cgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGAt                                                             <  1:1457420/124‑1 (MQ=255)                                                                                           aCTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                            >  2:1322334/1‑59 (MQ=255)                                                                                           aCTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                            <  1:1322334/59‑1 (MQ=255)                                                                                            cTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCaaa                                           >  1:743338/1‑139 (MQ=255)                                                                                             tttGAAAAACGCTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAAc                                          <  2:1074379/139‑1 (MQ=255)                                                                                                                 gTTGCTCAAGCGGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTaaa                      >  2:1527913/1‑139 (MQ=255)                                                                                                                 gTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                            <  2:1465421/37‑1 (MQ=255)                                                                                                                 gTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                            >  1:1465421/1‑37 (MQ=255)                                                                                                                  ttGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTaaaa                     >  1:1119896/1‑139 (MQ=255)                                                                                                                  ttGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTaaaa                     >  2:1270132/1‑139 (MQ=255)                                                                                                                   tGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAAt                    <  1:857385/139‑1 (MQ=255)                                                                                                                   tGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAAt                    >  1:436623/1‑139 (MQ=255)                                                                                                                          gcagcTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTa             <  2:867898/139‑1 (MQ=255)                                                                                                                              cTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTg                                                                                    >  2:1660/1‑64 (MQ=255)                                                                                                                              cTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTg                                                                                    <  1:1660/64‑1 (MQ=255)                                                                                                                                     aGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAc  <  2:743338/139‑1 (MQ=255)                                                                                                                                     aGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAc  <  1:1270132/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAC  >  NC_000913/923958‑924228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑