Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,407,462 T→C T112A (ACG→GCG)  ydaM ← diguanylate cyclase, csgD regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,407,4620TC100.0% 301.5 / NA 78T112A (ACG→GCG) ydaMdiguanylate cyclase, csgD regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/43);  total (35/43)

CATCGACGATTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGTTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGT  >  NC_000913/1407330‑1407592
                                                                                                                                    |                                                                                                                                  
cATCGACGATTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGc                                                                                                                                 <  2:162683/136‑1 (MQ=255)
cATCGACGATTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGc                                                                                                                                 >  1:162683/1‑136 (MQ=255)
cATCGACGATTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCgg                                                                                                                              <  2:575960/139‑1 (MQ=255)
 aTCGACGATTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGgc                                                                                                                             <  2:1419451/139‑1 (MQ=255)
   cgacgaTTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGgcgc                                                                                                                           <  1:38556/139‑1 (MQ=255)
     acgaTTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCtt                                                                                                                         >  1:1212878/1‑139 (MQ=255)
     acgaTTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCtt                                                                                                                         >  1:263096/1‑139 (MQ=255)
     acgaTTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAACTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCtt                                                                                                                         >  2:1162639/1‑139 (MQ=255)
        aTTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATCAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGa                                                                                                                      <  2:263096/139‑1 (MQ=255)
        aTTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAACATGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGa                                                                                                                      <  1:1162639/139‑1 (MQ=255)
          ttGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATa                                                                                                                    <  1:735236/139‑1 (MQ=255)
             tCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCt                                                                                                                 >  2:302325/1‑139 (MQ=255)
              ccATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCtt                                                                                                                <  2:1212878/139‑1 (MQ=255)
                    cGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGaaaa                                                                                                          <  1:1228503/139‑1 (MQ=255)
                        aCGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAaataat                                                                                                      <  1:667771/139‑1 (MQ=255)
                           ggTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAc                                                                                                   >  2:559405/1‑139 (MQ=255)
                            gTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCt                                                                                                                 <  1:1549456/124‑1 (MQ=255)
                            gTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCt                                                                                                                 >  2:1549456/1‑124 (MQ=255)
                            gTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAcc                                                                                                  >  2:1130066/1‑139 (MQ=255)
                            gTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAAAATCACCg                                                                                                 >  1:277269/1‑139 (MQ=255)
                                     caacaTTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGttctgtt                                                                                         >  1:510770/1‑139 (MQ=255)
                                              tGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGc                                                                                <  1:1473810/139‑1 (MQ=255)
                                                     ttAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCgg                                                                                                                              >  2:1345926/1‑86 (MQ=255)
                                                     ttAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCgg                                                                                                                              <  1:1345926/86‑1 (MQ=255)
                                                       aGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGgc                                                                                                                             <  1:1517207/85‑1 (MQ=255)
                                                       aGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGgc                                                                                                                             >  2:1517207/1‑85 (MQ=255)
                                                       aGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCgg                                                                                                <  2:1282512/114‑1 (MQ=255)
                                                       aGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCgg                                                                                                >  1:1282512/1‑114 (MQ=255)
                                                       aGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCaaa                                                                       >  2:289082/1‑139 (MQ=255)
                                                       aGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCaaa                                                                       >  1:1593024/1‑139 (MQ=255)
                                                        gTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCgg                                                                                                                              >  1:981027/1‑83 (MQ=255)
                                                        gTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCgg                                                                                                                              <  1:818713/83‑1 (MQ=255)
                                                        gTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCgg                                                                                                                              >  2:818713/1‑83 (MQ=255)
                                                        gTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCgg                                                                                                                              <  2:981027/83‑1 (MQ=255)
                                                        gTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAc                                                                                                   >  1:58836/1‑110 (MQ=255)
                                                        gTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAc                                                                                                   <  2:58836/110‑1 (MQ=255)
                                                        gTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCaaaa                                                                      >  1:1453409/1‑139 (MQ=255)
                                                        gTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCaaaa                                                                      >  1:592404/1‑139 (MQ=255)
                                                        gTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCaaaa                                                                      >  1:42141/1‑139 (MQ=255)
                                                               aaaaGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCg                                                               >  1:279457/1‑139 (MQ=255)
                                                                aaaGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGa                                                              <  1:1130066/139‑1 (MQ=255)
                                                                aaaGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGa                                                              <  2:1453409/139‑1 (MQ=255)
                                                                       gcATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGa                                                              <  2:463878/132‑1 (MQ=255)
                                                                       gcATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGa                                                              >  1:463878/1‑132 (MQ=255)
                                                                         aTAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAAt                                                     <  2:557228/139‑1 (MQ=255)
                                                                          tAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATg                                                    <  2:475194/139‑1 (MQ=255)
                                                                                 cTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGc                                                                                >  1:529653/1‑104 (MQ=255)
                                                                                 cTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGc                                                                                <  2:529653/104‑1 (MQ=255)
                                                                                   tGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAcc                                                                                                  <  1:600572/84‑1 (MQ=255)
                                                                                   tGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAcc                                                                                                  >  2:537276/1‑84 (MQ=255)
                                                                                   tGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAcc                                                                                                  >  2:600572/1‑84 (MQ=255)
                                                                                   tGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAcc                                                                                                  <  1:537276/84‑1 (MQ=255)
                                                                                   tGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCct                                           >  2:1502047/1‑139 (MQ=255)
                                                                                   tGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCct                                           <  2:1554939/139‑1 (MQ=255)
                                                                                   tGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCct                                           <  1:550254/139‑1 (MQ=255)
                                                                                    gTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAc                                                                                                   <  1:827554/82‑1 (MQ=255)
                                                                                    gTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAc                                                                                                   >  2:827554/1‑82 (MQ=255)
                                                                                        tttGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCtcttct                                      <  2:279457/139‑1 (MQ=255)
                                                                                           gcgcTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTa                                   >  1:1231143/1‑139 (MQ=255)
                                                                                             gcTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACg                                 <  2:1593024/139‑1 (MQ=255)
                                                                                             gcTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACg                                 <  1:4996/139‑1 (MQ=255)
                                                                                             gcTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACg                                 <  1:1502047/139‑1 (MQ=255)
                                                                                               tGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCt                               <  2:1231143/139‑1 (MQ=255)
                                                                                                 aTAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGa                             <  1:289082/139‑1 (MQ=255)
                                                                                                           gAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAc                                                                                                   >  1:268041/1‑59 (MQ=255)
                                                                                                           gAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAc                                                                                                   <  2:268041/59‑1 (MQ=255)
                                                                                                           gAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGa                                                              >  1:600615/1‑96 (MQ=255)
                                                                                                           gAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGa                                                              <  2:600615/96‑1 (MQ=255)
                                                                                                           gAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGa                   >  1:1328433/1‑139 (MQ=255)
                                                                                                           gAGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGa                   >  2:8701/1‑139 (MQ=255)
                                                                                                            aGCGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGac                  >  1:655894/1‑138 (MQ=255)
                                                                                                              cGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAc                                                                                                   <  2:371684/56‑1 (MQ=255)
                                                                                                              cGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCAc                                                                                                   >  1:371684/1‑56 (MQ=255)
                                                                                                              cGACTGGCTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGAttt                <  1:559405/139‑1 (MQ=255)
                                                                                                                     cTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGataat         <  2:510770/139‑1 (MQ=255)
                                                                                                                     cTTTTAAACCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGataat         <  1:302325/139‑1 (MQ=255)
                                                                                                                          aaaCCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCgt    <  1:884158/139‑1 (MQ=255)
                                                                                                                            aCCCAGCGCTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCgtgt  <  1:8701/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
CATCGACGATTTGTCCATCTCGTGACGGGTCAATCAACAACATTGGTGCAGAGTTAGTCAGAAAAAAGCGCGCATAAAAACCTTGTTTTTTGCGCTGATAATTTGCCGAGCGACTGGCTTTTAAACCCAGCGTTGCCGGCGCTTCGATACCTTCGAAAATAATCACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGT  >  NC_000913/1407330‑1407592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: