Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 164,205 A→G S701G (AGT→GGT)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,2050AG98.8% 319.5 / ‑3.4 85S701G (AGT→GGT) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (47/37);  minor base T (0/1);  total (47/38)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

TTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCC  >  NC_000913/164072‑164337
                                                                                                                                     |                                                                                                                                    
tttATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGaa                                                                                                                                 >  1:183156/1‑139 (MQ=255)
      ggCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAGATTACTCGACATTTATCAGGCACTCCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCt                                                                                                                           <  2:1011214/139‑1 (MQ=255)
        cAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTgc                                                                                                                         >  1:297940/1‑139 (MQ=255)
         aGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCt                                                                                                                           <  2:936912/136‑1 (MQ=255)
         aGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCt                                                                                                                           >  1:936912/1‑136 (MQ=255)
          gcgcTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTgcgc                                                                                                                       >  1:1022971/1‑139 (MQ=255)
          gcgcTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTgcgc                                                                                                                       >  1:1282574/1‑139 (MQ=255)
           cgcTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATCTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGGAACGTCTGGATGGTGAATTGCCTGCGCa                                                                                                                      <  2:1022971/139‑1 (MQ=255)
                gAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATTGTGAATTGCCTGCGCATTaca                                                                                                                 <  2:464094/139‑1 (MQ=255)
                      tGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGc                                                                                                           <  1:585894/139‑1 (MQ=255)
                       ggCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCt                                                                                                                           >  2:463756/1‑122 (MQ=255)
                       ggCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCt                                                                                                                           <  1:463756/122‑1 (MQ=255)
                                      tGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGAt                                                                                           <  1:982811/139‑1 (MQ=255)
                                       gACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATc                                                                                          >  1:627142/1‑139 (MQ=255)
                                         cTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCt                                                                                                                           >  2:431094/1‑104 (MQ=255)
                                         cTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCt                                                                                                                           <  1:431094/104‑1 (MQ=255)
                                            gCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACt                                                                                                               >  1:538474/1‑113 (MQ=255)
                                            gCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACt                                                                                                               <  2:538474/113‑1 (MQ=255)
                                            gCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCa                                                                                      >  1:649050/1‑138 (MQ=255)
                                            gCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCa                                                                                      <  2:649050/138‑1 (MQ=255)
                                            gCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCAt                                                                                     >  1:53982/1‑139 (MQ=255)
                                             cGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCAtt                                                                                    >  2:1253608/1‑139 (MQ=255)
                                             cGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCAtt                                                                                    >  1:1324525/1‑139 (MQ=255)
                                               tACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCg                                                                                  <  1:650581/139‑1 (MQ=255)
                                                 cATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAGTGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGtt                                                                                <  1:929648/139‑1 (MQ=255)
                                                 cATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGtt                                                                                >  1:1248735/1‑139 (MQ=255)
                                                 cATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGtt                                                                                >  1:1177118/1‑139 (MQ=255)
                                                 cATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGtt                                                                                >  1:1481062/1‑139 (MQ=255)
                                                      aCTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCg                                                                                  <  2:554450/132‑1 (MQ=255)
                                                      aCTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCg                                                                                  >  1:554450/1‑132 (MQ=255)
                                                       cTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATg                                                                          >  2:287311/1‑139 (MQ=255)
                                                       cTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATg                                                                          <  1:1253608/139‑1 (MQ=255)
                                                            cgGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGAt                                                                     >  1:174309/1‑139 (MQ=255)
                                                                         cTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCgcgc                                                        >  2:61668/1‑139 (MQ=255)
                                                                            gACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTgg                                                     <  2:743565/139‑1 (MQ=255)
                                                                                  tATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTga                                               <  2:174309/139‑1 (MQ=255)
                                                                                    tCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGa                                                                                                        >  2:767437/1‑80 (MQ=255)
                                                                                    tCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGa                                                                                                        <  1:767437/80‑1 (MQ=255)
                                                                                        gCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGc                                                    >  1:183280/1‑128 (MQ=255)
                                                                                        gCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGc                                                    <  2:183280/128‑1 (MQ=255)
                                                                                        gCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGc                                         >  2:695579/1‑139 (MQ=255)
                                                                                           cTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGAt                                                                                           >  1:1091790/1‑86 (MQ=255)
                                                                                           cTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGAt                                                                                           <  2:1091790/86‑1 (MQ=255)
                                                                                           cTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAag                                      <  2:1248735/139‑1 (MQ=255)
                                                                                            tACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAaga                                     >  2:757267/1‑139 (MQ=255)
                                                                                            tACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAaga                                     >  2:1212810/1‑139 (MQ=255)
                                                                                                  gATTACTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGtt                               >  2:1547933/1‑139 (MQ=255)
                                                                                                      aCTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCt                                                                                                                           <  1:1009099/43‑1 (MQ=255)
                                                                                                      aCTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTgag                                              >  2:1152666/1‑120 (MQ=255)
                                                                                                      aCTTGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTgag                                              <  1:1152666/120‑1 (MQ=255)
                                                                                                      aCTTGATAGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCt                                                                                                                           >  2:1009099/1‑43 (MQ=255)
                                                                                                        ttGATTGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGa                         <  1:757267/139‑1 (MQ=255)
                                                                                                             tGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAAcccgcccg                                                           >  1:1029314/1‑100 (MQ=255)
                                                                                                             tGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGcca                    >  2:1339346/1‑139 (MQ=255)
                                                                                                             tGGGGAATGCAGCAACGTCTGGATGGTGAATTGCCGGCGCATTACACTGTGGCGACGGGAAGCCGGATCGCCATTCGTGATCATGAAGATAAcccgcccg                                                           <  2:1029314/100‑1 (MQ=255)
                                                                                                              ggggAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGccac                   <  1:469999/139‑1 (MQ=255)
                                                                                                               gggAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATg                                                                          >  1:1219528/1‑83 (MQ=255)
                                                                                                               gggAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATg                                                                          <  2:1219528/83‑1 (MQ=255)
                                                                                                               gggAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCg                          <  2:749346/131‑1 (MQ=255)
                                                                                                               gggAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCg                          >  1:749346/1‑131 (MQ=255)
                                                                                                               gggAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGccacc                  <  1:1212810/139‑1 (MQ=255)
                                                                                                                ggAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAAcccgcc                                                             <  2:1640943/95‑1 (MQ=255)
                                                                                                                ggAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAAcccgcc                                                             >  1:1640943/1‑95 (MQ=255)
                                                                                                                 gAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCaa                >  2:1537363/1‑139 (MQ=255)
                                                                                                                 gAATGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCaa                >  1:759678/1‑139 (MQ=255)
                                                                                                                    tGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCg                                                                                  >  1:473020/1‑70 (MQ=255)
                                                                                                                    tGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCg                                                                                  <  2:473020/70‑1 (MQ=255)
                                                                                                                    tGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCa                                                                            <  1:386741/76‑1 (MQ=255)
                                                                                                                    tGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCa                                                                            >  2:386741/1‑76 (MQ=255)
                                                                                                                    tGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGc                           >  2:1641185/1‑125 (MQ=255)
                                                                                                                    tGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGc                           <  1:1641185/125‑1 (MQ=255)
                                                                                                                    tGCAGCAACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGTCACCAATcc             >  1:414268/1‑139 (MQ=255)
                                                                                                                         caACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGtt                                                                                >  1:1232359/1‑67 (MQ=255)
                                                                                                                         caACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGtt                                                                                <  2:1232359/67‑1 (MQ=255)
                                                                                                                         caACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCcgacga        <  2:53982/139‑1 (MQ=255)
                                                                                                                         caACGTCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCcgacga        <  2:1324525/139‑1 (MQ=255)
                                                                                                                              tCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGa                                                                                                   >  1:146087/1‑43 (MQ=255)
                                                                                                                              tCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGa                                                                                                   <  2:146087/43‑1 (MQ=255)
                                                                                                                              tCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTg                                                      >  1:1355654/1‑88 (MQ=255)
                                                                                                                              tCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTg                                                      <  2:1355654/88‑1 (MQ=255)
                                                                                                                              tCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGaa                                            <  1:871744/98‑1 (MQ=255)
                                                                                                                              tCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGaa                                            >  2:871744/1‑98 (MQ=255)
                                                                                                                              tCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGcc   >  2:1519060/1‑139 (MQ=255)
                                                                                                                              tCTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGcc   >  2:733178/1‑139 (MQ=255)
                                                                                                                               cTGGATGGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGccc  >  2:182633/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCC  >  NC_000913/164072‑164337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: