| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,141,143 | A→G | L157P (CTG→CCG) | asmA ← | suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,141,143 | 0 | A | G | 94.4% | 241.7 / 2.4 | 72 | L157P (CTG→CCG) | asmA | suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (31/37); total (33/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
GATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCTTCCGTCTGCGGCGTCAGT > NC_000913/2141008‑2141277 | gATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCa < 2:68045/139‑1 (MQ=255) tatCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCAACTGCTCGTCATCTTCATGCTGGAACACCGGCACg > 2:661116/1‑139 (MQ=255) atCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGc > 1:1128460/1‑139 (MQ=255) atCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGc < 1:874994/139‑1 (MQ=255) tCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCt > 1:590813/1‑139 (MQ=255) tGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTc > 1:1230363/1‑139 (MQ=255) gagaTCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTcc > 2:1607206/1‑139 (MQ=255) gaTCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc < 2:1603066/139‑1 (MQ=255) gaTCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc < 2:1230363/139‑1 (MQ=255) gaTCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc < 1:677859/139‑1 (MQ=255) gaTCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc < 1:1607206/139‑1 (MQ=255) cgcgCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCAc < 2:847338/139‑1 (MQ=255) cgcTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCt > 2:996387/1‑139 (MQ=255) cgcTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCt < 2:590813/139‑1 (MQ=255) cgcTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCt < 2:1450521/139‑1 (MQ=255) cgcTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCt < 1:996387/139‑1 (MQ=255) gcTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCtt < 1:113649/139‑1 (MQ=255) gcTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCtt < 2:1128460/139‑1 (MQ=255) gATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGa < 1:1120742/139‑1 (MQ=255) aaCCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGatata < 2:973654/139‑1 (MQ=255) aCCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGatatat > 2:1628865/1‑139 (MQ=255) cccgGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAAc > 2:212745/1‑139 (MQ=255) cgGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGa < 2:823838/139‑1 (MQ=255) cAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCcgcg < 2:842518/139‑1 (MQ=255) cAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCcgcg < 1:412929/139‑1 (MQ=255) cAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCACg < 1:1177558/95‑1 (MQ=255) cAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCACg > 2:1177558/1‑95 (MQ=255) aaTGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGAt < 2:1490852/139‑1 (MQ=255) ccACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTg > 2:1151185/1‑139 (MQ=255) aCGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGAc < 2:1453203/139‑1 (MQ=255) cGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACa > 2:97876/1‑139 (MQ=255) aTGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGa < 2:1165079/139‑1 (MQ=255) ggATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCa < 1:1339366/139‑1 (MQ=255) aTCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc > 1:296317/1‑81 (MQ=255) aTCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc < 2:296317/81‑1 (MQ=255) cTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCcgcg > 1:748043/1‑115 (MQ=255) cTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCcgcg < 2:748043/115‑1 (MQ=255) tGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc > 1:131682/1‑77 (MQ=255) tGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc < 2:131682/77‑1 (MQ=255) tGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTa < 1:214229/84‑1 (MQ=255) tGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTa > 2:214229/1‑84 (MQ=255) tGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGa > 2:27672/1‑87 (MQ=255) tGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGa < 1:27672/87‑1 (MQ=255) aTTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGa > 2:1259400/1‑98 (MQ=255) aTTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGa < 1:1259400/98‑1 (MQ=255) tttGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc > 1:274379/1‑70 (MQ=255) tttGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGc < 2:274379/70‑1 (MQ=255) tGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCgg > 2:520748/1‑122 (MQ=255) tGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCgg < 1:520748/122‑1 (MQ=255) tatTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCgg > 1:1236306/1‑139 (MQ=255) tGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCtt < 1:86733/61‑1 (MQ=255) tGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCtt > 2:86733/1‑61 (MQ=255) ttGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTc > 2:558918/1‑139 (MQ=255) tGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCcgcg < 2:273874/84‑1 (MQ=255) tGTCACCTGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCcgcg > 1:273874/1‑84 (MQ=255) tGCTCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCa < 2:494094/139‑1 (MQ=255) tCGTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCg > 2:341863/1‑139 (MQ=255) gTCATCTTCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCt < 2:624100/139‑1 (MQ=255) tCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGt > 2:1607663/1‑70 (MQ=255) tCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGt < 1:1607663/70‑1 (MQ=255) tCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATc < 1:155769/80‑1 (MQ=255) tCATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATc > 2:155769/1‑80 (MQ=255) cATGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCTTCCGTCTg > 1:1081811/1‑139 (MQ=255) aTGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTgg < 1:1308438/41‑1 (MQ=255) aTGCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTgg > 2:1308438/1‑41 (MQ=255) gCTGGAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCTTCCGTCTgcgg > 2:553281/1‑139 (MQ=255) gAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTg < 2:1584360/65‑1 (MQ=255) gAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTg > 1:1584360/1‑65 (MQ=255) gAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCTTCCGTCTGCGGCGTc > 1:1417479/1‑139 (MQ=255) gAACACCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCTTCCGTCTGCGGCGTc < 1:341863/139‑1 (MQ=255) cacCGGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCTTCCGTCTGCGGCGTCAGt > 1:164330/1‑139 (MQ=255) cacCAGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCTTCCGTCTGCGGCGTCAGt > 2:1226444/1‑139 (MQ=255) | GATATCGTGAGATCGCGCTGATCGCGATTAACCCGCCCGGAGAACTCAAATGAACCACGATGTTGGGGATCTTGTTCCATTTGCAGGCGAATATTGCGGATTGTCACCTGCTCGTCATCTTCATGCTGGAACACCAGCACGCTGTCCGCCACCTTAAGACTGGATATATCAAACGACCATCCGCGATCGTCTGACAGATCCGGCAAGGTATTGTCGCGCGGTGCAACCGGAGCGTCTTCACTGCGCACCGCTTCCGTCTGCGGCGTCAGT > NC_000913/2141008‑2141277 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |