| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,215,997 | A→G | R160R (CGT→CGC) | yehW ← | putative ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,215,997 | 0 | A | G | 81.8% | 76.5 / 10.2 | 33 | R160R (CGT→CGC) | yehW | putative ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (12/15); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01 | |||||||||||
GCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTG > NC_000913/2215859‑2216130 | gCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTa > 1:197861/1‑139 (MQ=255) tAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACg > 2:1037458/1‑139 (MQ=255) aaGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGc < 1:1374961/139‑1 (MQ=255) cGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCg < 2:302141/139‑1 (MQ=255) tACCCAGCGTACTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGCCCTTGCGCACTCGCTGACCACGACTCAt > 1:1120539/1‑139 (MQ=255) aCCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCAtt > 2:1447391/1‑139 (MQ=255) cGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATa < 2:945171/139‑1 (MQ=255) cGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATa > 1:674964/1‑139 (MQ=255) cTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAAc < 2:68427/139‑1 (MQ=255) aTCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCt < 1:207691/94‑1 (MQ=255) aTCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCt > 2:207691/1‑94 (MQ=255) aTCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTc > 2:270291/1‑105 (MQ=255) aTCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTc < 1:270291/105‑1 (MQ=255) tcgtcgCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGc < 1:1037458/139‑1 (MQ=255) gtcgCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCAt < 2:197861/139‑1 (MQ=255) aCCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGa < 2:1219284/91‑1 (MQ=255) aCCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGa > 1:1219284/1‑91 (MQ=255) tGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAg < 2:130917/139‑1 (MQ=255) aTAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTgcgc < 1:216939/66‑1 (MQ=255) aTAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTgcgc > 2:216939/1‑66 (MQ=255) aTAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCgg > 2:571782/1‑139 (MQ=255) tAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCa > 1:189251/1‑77 (MQ=255) tAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCa < 2:189251/77‑1 (MQ=255) tAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGc > 2:1173758/1‑139 (MQ=255) cGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTc < 1:571782/139‑1 (MQ=255) aaGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGc > 1:861201/1‑139 (MQ=255) cGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGAc > 2:1517693/1‑67 (MQ=255) cGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGAc < 1:1517693/67‑1 (MQ=255) cGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCa > 2:264195/1‑139 (MQ=255) cgcccgccAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGAc < 2:1205590/139‑1 (MQ=255) ccgccAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACggg < 1:1447391/139‑1 (MQ=255) aGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGgcagca < 1:712178/139‑1 (MQ=255) ccGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACGCCGTa < 1:264195/139‑1 (MQ=255) ccGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTa < 2:674964/139‑1 (MQ=255) ggTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGa < 1:1173758/139‑1 (MQ=255) aCCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTg > 2:940863/1‑139 (MQ=255) | GCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTG > NC_000913/2215859‑2216130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |