| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,253,271 | G→A | A292V (GCC→GTC) | nupX ← | nucleoside permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,253,271 | 0 | G | A | 92.6% | 159.6 / 3.8 | 54 | A292V (GCC→GTC) | nupX | nucleoside permease |
| Reads supporting (aligned to +/- strand): ref base G (1/3); new base A (28/22); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
TGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGTCATTGCCCCTGTCGCAG > NC_000913/2253137‑2253403 | tGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATg > 2:1613209/1‑139 (MQ=255) agaTAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGtt < 1:1613209/139‑1 (MQ=255) tGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATTACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAAcg < 2:493341/139‑1 (MQ=255) tttCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGccgccg > 1:487930/1‑139 (MQ=255) tGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataa < 1:131478/139‑1 (MQ=255) tCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAaaaccaaac < 1:1116317/110‑1 (MQ=255) tCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAaaaccaaac > 2:1116317/1‑110 (MQ=255) cTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAAcgcc < 2:1441830/119‑1 (MQ=255) cTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAAcgcc < 2:120302/119‑1 (MQ=255) cTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAAcgcc > 1:120302/1‑119 (MQ=255) cTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAAcgcc > 1:1441830/1‑119 (MQ=255) ggCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCa < 2:1515007/139‑1 (MQ=255) ggCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataa < 2:1532061/122‑1 (MQ=255) ggCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataa > 1:1532061/1‑122 (MQ=255) gCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCaa < 2:1154032/139‑1 (MQ=255) aTTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAAACTAAAATGGGCTCAAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAAGGCAattatt > 1:1124729/1‑139 (MQ=37) cATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGcaac < 2:635120/139‑1 (MQ=255) tCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGcaacaa < 2:226584/139‑1 (MQ=255) aCTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAAt < 2:178341/139‑1 (MQ=255) cAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCg < 2:856057/108‑1 (MQ=255) cAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCg > 1:856057/1‑108 (MQ=255) acCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataa < 1:637529/95‑1 (MQ=255) acCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGataa > 2:637529/1‑95 (MQ=255) acCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAAt < 1:1086408/105‑1 (MQ=255) acCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAAt > 2:1086408/1‑105 (MQ=255) acCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACt > 1:1115587/1‑139 (MQ=255) tCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAAcgcc < 1:632554/82‑1 (MQ=255) tCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAAcgcc > 2:632554/1‑82 (MQ=255) tCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAaaccaaacca < 2:1025085/70‑1 (MQ=255) tCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAaaccaaacca > 1:1025085/1‑70 (MQ=255) cACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCacac > 1:1108324/1‑139 (MQ=255) cGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATcaccac < 2:694686/126‑1 (MQ=255) cGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATcaccac > 1:694686/1‑126 (MQ=255) cGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCt > 1:475154/1‑139 (MQ=255) cGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCt > 2:172217/1‑139 (MQ=255) gCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTg > 2:93657/1‑139 (MQ=255) aGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTgcgg > 2:1092694/1‑139 (MQ=255) ccAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAAcg > 1:113365/1‑62 (MQ=255) ccAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAAcg < 2:113365/62‑1 (MQ=255) ccAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAAc > 1:1193688/1‑86 (MQ=255) ccAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAAc < 2:1193688/86‑1 (MQ=255) ccAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGAtttt > 2:938895/1‑139 (MQ=255) ccAGCAGGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGAtttt > 1:185907/1‑139 (MQ=255) cagGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGc > 1:732933/1‑139 (MQ=255) agGTAACCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGcc < 2:1108324/139‑1 (MQ=255) aaCCTAAAATGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGt > 1:126812/1‑139 (MQ=255) aaaaTGGACTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGACATTg > 1:411702/1‑139 (MQ=255) aCTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAAcg < 2:1494249/41‑1 (MQ=255) aCTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAAcg > 1:1494249/1‑41 (MQ=255) aCTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTg < 2:1074075/75‑1 (MQ=255) aCTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTg > 1:1074075/1‑75 (MQ=255) aCTCCAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGTCATTGCCCCTGt > 2:119343/1‑139 (MQ=255) cAGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGTCATTGCCCCTGTCGCa < 1:119343/139‑1 (MQ=255) aGCGAGACATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGTCATTGCCCCTGTCGCAg < 1:172217/139‑1 (MQ=255) | TGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGTCATTGCCCCTGTCGCAG > NC_000913/2253137‑2253403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |