Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,284,097 T→C intergenic (‑150/‑32) yejK ← / → yejL spermidine nucleoid‑associated protein/UPF0352 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,284,0970TC93.8% 157.3 / ‑0.4 48intergenic (‑150/‑32)yejK/yejLspermidine nucleoid‑associated protein/UPF0352 family protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (22/23);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TTAAGACCGGGCGGTATTCAACCACCGCCGGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGTTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGA  >  NC_000913/2283961‑2284231
                                                                                                                                        |                                                                                                                                      
ttAAGACCGGGCGGTATTCAACCACCGCCGGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTg                                                                                                                                      >  2:497277/1‑139 (MQ=255)
  aaGACCGGGCGGTATTCAACCACCGCCGGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGcc                                                                                                                                    <  1:1395845/139‑1 (MQ=255)
        gggCGGTATTCAACCACCGCCGGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGTTGCCCTTtca                                                                                                                              >  2:286610/1‑139 (MQ=255)
        gggCGGTATTCAACCACCGCCGGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGcc                                                                                                                                    >  2:239485/1‑133 (MQ=255)
        gggCGGTATTCAACCACCGCCGGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGcc                                                                                                                                    <  1:239485/133‑1 (MQ=255)
         ggCGGTATTCAACCACCGCCGGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTtcat                                                                                                                             >  1:843630/1‑139 (MQ=255)
                        ccgccgGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTc                                                                                                              >  2:524180/1‑139 (MQ=255)
                        ccgccgGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTc                                                                                                              >  2:406567/1‑139 (MQ=255)
                                                 gATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGTTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTa                                                                                     <  1:1481857/139‑1 (MQ=255)
                                                    ggCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAg                                                                                  >  2:695220/1‑139 (MQ=255)
                                                     gCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGTTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGt                                                                                 <  1:286610/139‑1 (MQ=255)
                                                      cGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGAAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGtg                                                                                >  1:294448/1‑139 (MQ=255)
                                                            ttttACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAAc                                                                          <  1:497277/139‑1 (MQ=255)
                                                                      ggCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCttt                                                                                                                                >  2:877622/1‑75 (MQ=255)
                                                                      ggCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCttt                                                                                                                                <  1:877622/75‑1 (MQ=255)
                                                                      ggCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTAt                                                                                                       <  2:434554/100‑1 (MQ=255)
                                                                      ggCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTAt                                                                                                       >  1:434554/1‑100 (MQ=255)
                                                                      ggCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTcc                                                                                          <  1:938536/113‑1 (MQ=255)
                                                                      ggCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTcc                                                                                          >  2:938536/1‑113 (MQ=255)
                                                                       gCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACaa                                                               >  2:613476/1‑139 (MQ=255)
                                                                         aGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAAtt                                                             >  1:387601/1‑139 (MQ=255)
                                                                              aaCGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCg                                                        <  2:843630/139‑1 (MQ=255)
                                                                              aaCGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCg                                                        <  1:524180/139‑1 (MQ=255)
                                                                                cGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCa                                                      <  1:613476/139‑1 (MQ=255)
                                                                                 gagaATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCag                                                     <  2:468616/139‑1 (MQ=255)
                                                                                 gagaATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCag                                                     <  2:499698/139‑1 (MQ=255)
                                                                                  agaATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCttt                                                                                                                                >  2:1422696/1‑63 (MQ=255)
                                                                                  agaATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCttt                                                                                                                                <  1:1422696/63‑1 (MQ=255)
                                                                                  agaATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCaga                                                    >  2:1211712/1‑139 (MQ=255)
                                                                                     aTGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCaactaa                                                                                                                      >  2:604346/1‑70 (MQ=255)
                                                                                     aTGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCaactaa                                                                                                                      <  1:604346/70‑1 (MQ=255)
                                                                                         tttAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTc                                             >  2:150253/1‑139 (MQ=255)
                                                                                          ttAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCa                                            <  2:541561/139‑1 (MQ=255)
                                                                                           tAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAgctg                                                >  2:1595031/1‑134 (MQ=255)
                                                                                           tAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAgctg                                                <  1:1595031/134‑1 (MQ=255)
                                                                                                  tAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTg                                    >  2:26118/1‑139 (MQ=255)
                                                                                                     aaaaGCAGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGaa                                 >  1:728417/1‑139 (MQ=255)
                                                                                                          cAGAAAAAAAACCGTTGCTACGGTAATATGCTGcc                                                                                                                                    <  2:209626/35‑1 (MQ=255)
                                                                                                          cAGAAAAAAAACCGTTGCTACGGTAATATGCTGcc                                                                                                                                    >  1:209626/1‑35 (MQ=255)
                                                                                                           aGAAAAAAAACCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACAt                           <  1:1471063/139‑1 (MQ=255)
                                                                                                             aaaaaaaaCCGTTGCTACGGTAATATGCTGCCCTTTCATCaactaa                                                                                                                      <  1:377200/46‑1 (MQ=255)
                                                                                                             aaaaaaaaCCGTTGCTACGGTAATATGCTGCCCTTTCATCaactaa                                                                                                                      >  2:377200/1‑46 (MQ=255)
                                                                                                             aaaaaaaaCCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGtga                                                                               >  2:503996/1‑85 (MQ=255)
                                                                                                             aaaaaaaaCCGTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGtga                                                                               <  1:503996/85‑1 (MQ=255)
                                                                                                                       gTTGCTACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTccgacc               <  1:1211712/139‑1 (MQ=255)
                                                                                                                            tACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCt          <  2:387601/139‑1 (MQ=255)
                                                                                                                            tACGGTAATATGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCt          <  2:1434053/139‑1 (MQ=255)
                                                                                                                                    tatGCTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGa  <  1:406567/139‑1 (MQ=255)
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TTAAGACCGGGCGGTATTCAACCACCGCCGGTTAAGCGACGCAATAAAAGATGGCGGTCATTTTACAGAAGGCAGCGTAACGAGAATGATTTAACGCATAAAAAAGCAGAAAAAAAACCGTTGCTACGGTAATATGTTGCCCTTTCATCAACTAACTGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGA  >  NC_000913/2283961‑2284231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: