Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,830,088 G→A V92V (GTG→GTA)  srlQ → D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,830,0880GA84.3% 122.8 / 12.9 51V92V (GTG→GTA) srlQD‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (5/3);  new base A (25/18);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGGGAATTGGCAAATCGGGCCACATTGGTAAGAAAATCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAA  >  NC_000913/2829953‑2830215
                                                                                                                                       |                                                                                                                               
ttCGGGAATTGGCAAATCGGGCCACATTGGTAAGAAAATCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTTGGGATGATCGAAAGCCGCGATGTAATg                                                                                                                              <  1:982308/139‑1 (MQ=255)
ttCGGGAATTGGCAAATCGGGCCACATTGGTAAGAAAATCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATg                                                                                                                              <  2:420964/139‑1 (MQ=255)
   gggAATTGGCAAATCGGGCCACATTGGTAAGAAAATCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTg                                                                                                                           >  2:1151356/1‑139 (MQ=255)
   gggAATTGGCAAATCGGGCCACATTGGTAAGAAAATCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTg                                                                                                                           >  1:1642717/1‑139 (MQ=255)
      aaTTGGCAAATCGGGCCACATTGGTAAGAAAATCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGttt                                                                                                                        <  2:856015/139‑1 (MQ=255)
           gCAAATCGGGCCACATTGGTAAGAAAATCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTAtctc                                                                                                                   >  2:560703/1‑139 (MQ=255)
                            ggTAAGAAAATCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGa                                                                                                  <  2:1379488/139‑1 (MQ=255)
                                  aaaaTCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGCTGATCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAAc                                                                                            >  2:236239/1‑139 (MQ=255)
                                      tCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGa                                                                                        <  1:1151356/139‑1 (MQ=255)
                                       cgccgcAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTg                                                                                          <  2:564745/136‑1 (MQ=255)
                                       cgccgcAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTg                                                                                          >  1:564745/1‑136 (MQ=255)
                                       cgccgcAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGttt                                                                                                                        <  1:323006/106‑1 (MQ=255)
                                       cgccgcAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGttt                                                                                                                        >  2:323006/1‑106 (MQ=255)
                                                         tACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTAtct                                                                                                                    <  1:1543936/92‑1 (MQ=255)
                                                         tACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTAtct                                                                                                                    >  2:1543936/1‑92 (MQ=255)
                                                              gCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATg                                                                                                                              <  1:487812/77‑1 (MQ=255)
                                                              gCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATg                                                                                                                              >  2:487812/1‑77 (MQ=255)
                                                              gCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGa                                                                >  1:862760/1‑139 (MQ=255)
                                                                aCTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGaa                                                                                                 <  1:777603/104‑1 (MQ=255)
                                                                aCTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGaa                                                                                                 >  2:777603/1‑104 (MQ=255)
                                                                       cTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGaa                                                                                             >  2:244031/1‑101 (MQ=255)
                                                                       cTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGaa                                                                                             <  1:244031/101‑1 (MQ=255)
                                                                                    tCCGGCAGAAGCGCTCCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGATCTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCg                                          >  1:1099549/1‑139 (MQ=255)
                                                                                     ccGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATg                                                                                                                              >  1:548957/1‑54 (MQ=255)
                                                                                     ccGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATg                                                                                                                              <  2:548957/54‑1 (MQ=255)
                                                                                     ccGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGcgcg                                                                                                   <  2:449616/81‑1 (MQ=255)
                                                                                     ccGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGcgcg                                                                                                   >  1:449616/1‑81 (MQ=255)
                                                                                                 cTGCATGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGt                                                                                                                          <  1:1344383/46‑1 (MQ=255)
                                                                                                 cTGCATGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGt                                                                                                                          >  2:1344383/1‑46 (MQ=255)
                                                                                                  tGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAAcc                            >  1:559001/1‑139 (MQ=255)
                                                                                                   gCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAAc                                                                                            <  2:221795/74‑1 (MQ=255)
                                                                                                   gCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAAc                                                                                            >  1:221795/1‑74 (MQ=255)
                                                                                                    cACGGCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGa                          >  2:1263824/1‑139 (MQ=255)
                                                                                                     aCGGCGATCTGGGGATGATCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGa                                                                                        <  1:169731/76‑1 (MQ=255)
                                                                                                     aCGGCGATCTGGGGATGATCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGa                                                                                        >  2:169731/1‑76 (MQ=255)
                                                                                                       ggCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAAc                                                                                            <  2:338077/70‑1 (MQ=255)
                                                                                                       ggCGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAAc                                                                                            >  1:338077/1‑70 (MQ=255)
                                                                                                         cGATCTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCa                     >  1:1094448/1‑139 (MQ=255)
                                                                                                             cTGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGc                 <  2:559001/139‑1 (MQ=255)
                                                                                                              tGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGa                                                                                                  <  2:679794/57‑1 (MQ=255)
                                                                                                              tGGGGATGATCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGa                                                                                                  >  1:679794/1‑57 (MQ=255)
                                                                                                                      atCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCgcgc                                                  >  2:34438/1‑97 (MQ=255)
                                                                                                                      atCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCgcgc                                                  <  1:34438/97‑1 (MQ=255)
                                                                                                                      atCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTgg        >  1:106447/1‑139 (MQ=255)
                                                                                                                      atCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTgg        >  1:817039/1‑139 (MQ=255)
                                                                                                                      atCGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGAT‑AATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCAACGCTGGGCCTggc       >  1:601694/1‑139 (MQ=255)
                                                                                                                        cGAAAGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGCCCGGCAAACCGACGTCACCGCTGGGCCTggcg      >  2:1376362/1‑139 (MQ=255)
                                                                                                                          aaaGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTggcggc    >  2:434266/1‑139 (MQ=255)
                                                                                                                            aGCCGCGATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCaa  >  2:1339981/1‑139 (MQ=255)
                                                                                                                                 cgATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGa                                                                                                  <  1:219730/38‑1 (MQ=255)
                                                                                                                                 cgATGTAATGCTGTTTATCTCTTACTCCGGTGGCGCGa                                                                                                  >  2:219730/1‑38 (MQ=255)
                                                                                                                                       |                                                                                                                               
TTCGGGAATTGGCAAATCGGGCCACATTGGTAAGAAAATCGCCGCAACGCTTGCCAGTACCGGCACTCCGGCTTTTTTTGTCCATCCGGCAGAAGCGCTGCACGGCGATCTGGGGATGATCGAAAGCCGCGATGTGATGCTGTTTATCTCTTACTCCGGTGGCGCGAAGGAACTGGATCTGATTATTCCGCGTCTGGAAGATAAATCTATCGCGCTGCTGGCGATGACCGGCAAACCGACGTCACCGCTGGGCCTGGCGGCAA  >  NC_000913/2829953‑2830215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑