Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,895,747	C→T	E11E (GAG→GAA)	ygcQ ←	putative flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,895,747	0	C	T	100.0%	199.7 / NA	57	E11E (GAG→GAA)	ygcQ	putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/32);  total (25/32)

GGCGTTCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCT  >  NC_000913/2895613‑2895873                                                                                                                                       |                                                                                                                               ggCGTTCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCt                                                                                                                            <  2:776497/139‑1 (MQ=255)              aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTcc                                                                                                                             <  1:871017/125‑1 (MQ=255)              aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTcc                                                                                                                             >  2:871017/1‑125 (MQ=255)                tGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACaa                                                                                                             <  1:1501880/139‑1 (MQ=255)                  ttCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAAtt                                                                                                           >  1:888617/1‑139 (MQ=255)                   tCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTg                                                                                                          >  2:1562671/1‑139 (MQ=255)                     cgcgaaCGCATCCGGCACCTGTGCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGTTATAACCGCGGAAAGTCCGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCg                                                                                                        <  1:922640/135‑1 (MQ=255)                     caccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCg                                                                                                        <  1:137893/139‑1 (MQ=255)                      accaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGa                                                                                                       >  2:1017985/1‑139 (MQ=255)                       ccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGat                                                                                                      >  1:1148204/1‑139 (MQ=255)                         aaCGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGatat                                                                                                    >  1:450764/1‑139 (MQ=255)                             cATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCAt                                                                                                <  1:1017985/139‑1 (MQ=255)                                    cACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCa                                                                                         >  2:1289242/1‑139 (MQ=255)                                    cACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCa                                                                                         >  2:965004/1‑139 (MQ=255)                                    cACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCa                                                                                         <  1:818465/139‑1 (MQ=255)                                       cagTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATcc                                                                                      >  1:161673/3‑139 (MQ=255)                                        tGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                     <  2:1464312/139‑1 (MQ=255)                                        tGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                     <  1:127532/139‑1 (MQ=255)                                        tGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCt                                                                                     <  2:314658/139‑1 (MQ=255)                                         gTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCtt                                                                                    <  1:1289242/139‑1 (MQ=255)                                              gCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCg                                                                               >  2:1456962/1‑139 (MQ=255)                                               ccACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATtttc                                                                                                                              <  2:1259062/91‑1 (MQ=255)                                                  cTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTaaa                                                                           <  2:650829/139‑1 (MQ=255)                                                      aGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGt                                                                       <  2:1563949/139‑1 (MQ=255)                                                       ggCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                      <  1:538781/139‑1 (MQ=255)                                                       ggCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                      >  1:1401354/1‑139 (MQ=255)                                                       ggCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTc                                                                      <  1:1271584/139‑1 (MQ=255)                                                            aGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGcc                                                                 >  2:1512428/1‑139 (MQ=255)                                                            aGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGcc                                                                 >  2:1301071/1‑139 (MQ=255)                                                             ggTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCa                                                                >  2:688135/1‑139 (MQ=255)                                                                  aaTCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCt                                                           >  2:1609780/1‑139 (MQ=255)                                                                  aaTCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCt                                                           >  2:466339/1‑139 (MQ=255)                                                                        ccAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAg                                                     <  2:1401354/139‑1 (MQ=255)                                                                          aGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCt                                                   <  1:688135/139‑1 (MQ=255)                                                                            tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCa                                                                                                 <  1:789884/91‑1 (MQ=255)                                                                            tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCa                                                                                                 >  2:789884/1‑91 (MQ=255)                                                                            tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGc                                                                                >  2:1027090/1‑108 (MQ=255)                                                                            tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGc                                                                                <  1:1027090/108‑1 (MQ=255)                                                                            tGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCttt                                                 >  1:799405/1‑139 (MQ=255)                                                                              cgcCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTcac                                                                                                               <  1:723583/75‑1 (MQ=255)                                                                              cgcCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTcac                                                                                                               >  2:723583/1‑75 (MQ=255)                                                                              cgcCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACaa                                                                                                             <  1:617213/77‑1 (MQ=255)                                                                              cgcCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACaa                                                                                                             >  2:617213/1‑77 (MQ=255)                                                                              cgcCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCttttt                                               <  2:799405/139‑1 (MQ=255)                                                                                   gggTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCt                                          <  2:888617/139‑1 (MQ=255)                                                                                   gggTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCt                                          <  1:1301071/139‑1 (MQ=255)                                                                                   gggTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCt                                          <  1:1512428/139‑1 (MQ=255)                                                                                        cAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGt                                                                                                                  >  2:1567030/1‑62 (MQ=255)                                                                                        cAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGt                                                                                                                  <  1:1567030/62‑1 (MQ=255)                                                                                              cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCg                                                                                                        <  2:517943/66‑1 (MQ=255)                                                                                              cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCg                                                                                                        >  1:517943/1‑66 (MQ=255)                                                                                                         cgccgcCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAAt                                                                                                            >  2:489397/1‑51 (MQ=255)                                                                                                         cgccgcCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAAt                                                                                                            <  1:489397/51‑1 (MQ=255)                                                                                                         cgccgcCAGCCAGCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCg                    >  1:1329173/1‑139 (MQ=255)                                                                                                              ccagccagCTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCt               <  2:161673/139‑1 (MQ=255)                                                                                                              ccagccagCATGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCt               <  2:1056201/139‑1 (MQ=255)                                                                                                                      cTTGCTATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGcgtcg       <  1:1456962/139‑1 (MQ=255)                                                                                                                           tATTGCGGCATTTTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgct  <  2:1148204/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                               GGCGTTCGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCT  >  NC_000913/2895613‑2895873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑