Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,139,583	G→A	R286H (CGT→CAT)	yghU →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,139,583	0	G	A	100.0%	187.4 / NA	55	R286H (CGT→CAT)	yghU	putative S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/23);  total (32/23)

CGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCAT  >  NC_000913/3139447‑3139716                                                                                                                                         |                                                                                                                                      cGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGGTAAGCATc                                                                                                                                     <  2:1220764/139‑1 (MQ=255) cGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                     <  1:64172/139‑1 (MQ=255) cGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                     <  1:210455/139‑1 (MQ=255)   cTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                     <  2:765406/137‑1 (MQ=255)   cTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                     >  1:765406/1‑137 (MQ=255)           aagaagTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAgg                                                                                                                           >  2:896823/1‑139 (MQ=255)                   ggCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                     <  1:1002708/121‑1 (MQ=255)                    gCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGGCGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGtt                                                                                                                  >  2:1004784/1‑139 (MQ=255)                       aaCGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGGCGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTtcgt                                                                                                               <  1:1004784/139‑1 (MQ=255)                                                        aCCGCACCAACGGACCGGTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGAAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTc                                                                              >  1:945803/1‑139 (MQ=255)                                                           gCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcg                                                                           >  2:270496/1‑139 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                               <  2:479164/86‑1 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                               >  1:479164/1‑86 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCgcagca                                                                                                        >  1:786922/1‑109 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCgcagca                                                                                                        <  2:786922/109‑1 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCgc                                                                                                            <  1:424435/105‑1 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCgc                                                                                                            >  2:424435/1‑105 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAtgc                                                                          >  1:1526215/1‑139 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgc                                                                          >  1:1043786/1‑139 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgc                                                                          >  2:1177807/1‑139 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgc                                                                          >  1:1377221/1‑139 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGGGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgc                                                                          >  1:983407/1‑139 (MQ=255)                                                             aCCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgcc                                                                         >  2:738379/1‑139 (MQ=255)                                                              ccAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgccg                                                                        <  2:1126867/139‑1 (MQ=255)                                                                         cTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCAt                                                                                                  >  1:1306582/1‑102 (MQ=255)                                                                         cTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCAt                                                                                                  <  2:1306582/102‑1 (MQ=255)                                                                         cTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt                                                             >  2:376720/1‑139 (MQ=255)                                                                          tGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAATc                                                            <  1:896823/139‑1 (MQ=255)                                                                                 gCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcaca                                                     >  2:1267900/1‑139 (MQ=255)                                                                                 gCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcaca                                                     >  2:1366579/1‑139 (MQ=255)                                                                                      tGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGcc                                                >  2:613754/1‑139 (MQ=255)                                                                                      tGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGcc                                                >  1:283695/1‑139 (MQ=255)                                                                                          tGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                               <  2:436553/56‑1 (MQ=255)                                                                                          tGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                               >  1:436553/1‑56 (MQ=255)                                                                                             gcgcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCg                                         >  1:115803/1‑139 (MQ=255)                                                                                              cgcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGa                                        >  1:1189739/1‑139 (MQ=255)                                                                                               gcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt                                                             <  2:1369574/117‑1 (MQ=255)                                                                                               gcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt                                                             >  1:1369574/1‑117 (MQ=255)                                                                                               gcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGAc                                       <  2:283695/139‑1 (MQ=255)                                                                                                    gACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGc                                  <  2:983407/139‑1 (MQ=255)                                                                                                      cGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                     <  1:926219/38‑1 (MQ=255)                                                                                                      cGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                     >  2:926219/1‑38 (MQ=255)                                                                                                       gCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                               >  2:524016/1‑43 (MQ=255)                                                                                                       gCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                               <  1:524016/43‑1 (MQ=255)                                                                                                       gCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCcgc                               >  2:503355/1‑139 (MQ=255)                                                                                                         cAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGt                             <  2:1377221/139‑1 (MQ=255)                                                                                                               tttCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                               <  2:172043/35‑1 (MQ=255)                                                                                                               tttCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                               >  1:172043/1‑35 (MQ=255)                                                                                                                  cGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAgg                    <  1:1143505/139‑1 (MQ=255)                                                                                                                   gagaCGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGc                   <  1:503355/139‑1 (MQ=255)                                                                                                                      aCGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTa                >  1:831335/1‑139 (MQ=255)                                                                                                                        gAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACg              >  2:1287560/1‑139 (MQ=255)                                                                                                                                   aTACGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatca   >  2:1121639/1‑139 (MQ=255)                                                                                                                                    tAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat  <  2:831335/139‑1 (MQ=255)                                                                                                                                    tAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat  >  1:284280/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                      CGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCAT  >  NC_000913/3139447‑3139716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑