Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,218,665 T→C T138A (ACC→GCC)  aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,218,6650TC83.7% 122.3 / 14.7 49T138A (ACC→GCC) aerfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base T (3/5);  new base C (20/21);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

ATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCAC  >  NC_000913/3218541‑3218794
                                                                                                                            |                                                                                                                                 
aTGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCa                                                                                                                     <  2:782060/139‑1 (MQ=255)
   gccgccAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACg                                                                                                                  >  1:512559/1‑139 (MQ=255)
     cgccAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCt                                                                                                                <  1:541753/139‑1 (MQ=255)
       ccAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCttt                                                                                                              >  1:1252904/1‑139 (MQ=255)
        cAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTg                                                                                                             <  1:1447753/139‑1 (MQ=255)
                    cATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTcc                                                                                                 <  1:583499/139‑1 (MQ=255)
                         gggTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCAccgc                                                                                            >  2:1566920/1‑139 (MQ=255)
                         gggTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCAccgc                                                                                            >  1:745447/1‑139 (MQ=255)
                          ggTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCTAGCCAGCCTTTACGCACCACCAGACCTTTATGAATACGCGTACTGGTACGACCGGCGTTCAACGCTTTGTACAGCGGCTCCAccgcc                                                                                           <  2:1048011/139‑1 (MQ=255)
                              atcaCTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGa                                                                                       >  1:290464/1‑139 (MQ=255)
                                          gcccgccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCg                                                                           >  2:424615/1‑139 (MQ=255)
                                          gcccgccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCg                                                                           >  1:1173751/1‑139 (MQ=255)
                                           cccgccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGt                                                                          <  2:1173751/139‑1 (MQ=255)
                                             cgccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCg                                                                        <  2:290464/139‑1 (MQ=255)
                                               ccAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGcc                                                                      >  2:333754/1‑139 (MQ=255)
                                                          gTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAAt                                                           <  2:1255740/139‑1 (MQ=255)
                                                          gTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAAt                                                           <  1:59917/139‑1 (MQ=255)
                                                           tAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATc                                                          <  2:695212/139‑1 (MQ=255)
                                                                     aGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGc                                                >  1:434734/1‑139 (MQ=255)
                                                                                    ccTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCt                                 >  2:458371/1‑139 (MQ=255)
                                                                                       ttACGCACCACCAGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTcgc                              <  1:70616/139‑1 (MQ=255)
                                                                                              ccaccaGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt                       <  2:758758/139‑1 (MQ=255)
                                                                                                accaGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTAc                                                                                                          <  1:911610/54‑1 (MQ=255)
                                                                                                accaGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTAc                                                                                                          >  2:911610/1‑54 (MQ=255)
                                                                                                 ccaGGCCTTTATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCgg                    >  2:758309/1‑139 (MQ=255)
                                                                                                   aGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTa                  <  1:1250757/139‑1 (MQ=255)
                                                                                                         ttATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt                       >  1:1496524/1‑128 (MQ=255)
                                                                                                         ttATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt                       <  2:1496524/128‑1 (MQ=255)
                                                                                                         ttATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGaa                                                            >  2:117050/1‑91 (MQ=255)
                                                                                                         ttATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGaa                                                            <  1:117050/91‑1 (MQ=255)
                                                                                                         ttATGAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCAt            >  2:261068/1‑139 (MQ=255)
                                                                                                         ttATGAATACCCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt                       <  2:44125/128‑1 (MQ=255)
                                                                                                         ttATGAATACCCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt                       >  1:44125/1‑128 (MQ=255)
                                                                                                             gAATACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTAAGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGc        <  1:320563/139‑1 (MQ=255)
                                                                                                               aTACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGccc      <  2:611082/139‑1 (MQ=255)
                                                                                                                tACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCg     >  1:1393092/1‑139 (MQ=255)
                                                                                                                tACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTcc                                   <  2:912878/109‑1 (MQ=255)
                                                                                                                tACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTcc                                   >  1:912878/1‑109 (MQ=255)
                                                                                                                tACGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCg     <  2:291081/139‑1 (MQ=255)
                                                                                                                  cGCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCa   <  2:996748/139‑1 (MQ=255)
                                                                                                                   gCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGccc      >  2:237481/1‑135 (MQ=255)
                                                                                                                   gCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGccc      <  1:237481/135‑1 (MQ=255)
                                                                                                                   gCTTACTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCAc  >  2:1368520/1‑139 (MQ=255)
                                                                                                                       aCTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATGGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCa   <  2:942421/134‑1 (MQ=255)
                                                                                                                       aCTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCa   >  1:942421/1‑134 (MQ=255)
                                                                                                                        cTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATc                                                          <  1:1546020/78‑1 (MQ=255)
                                                                                                                        cTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATc                                                          >  2:1546020/1‑78 (MQ=255)
                                                                                                                        cTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt                       <  2:1223589/113‑1 (MQ=255)
                                                                                                                        cTGGCACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCAt                       >  1:1223589/1‑113 (MQ=255)
                                                                                                                            |                                                                                                                                 
ATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATACGCTTACTGGTACGTCCGGCGTTCAACGCTTTGTACAGCGGCTCCACCGCCGCGATCTCTTCATCCGTCGCCCGGGTACGAATCGACATATAGCCACTGATTTTTCCCTCGCGCACCATCGGTACCGCATTGGCCCGCAC  >  NC_000913/3218541‑3218794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: