Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	322,098	C→T	T164I (ACC→ATC)	ykgE →	cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	322,098	0	C	T	100.0%	268.0 / NA	78	T164I (ACC→ATC)	ykgE	cysteine‑rich LutA family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/38);  total (40/38)

TAGTCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGACCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTG  >  NC_000913/321969‑322229                                                                                                                                  |                                                                                                                                    tAGTCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGa                                                                                                                            <  2:1005654/139‑1 (MQ=255) tAGTCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGa                                                                                                                            <  1:1600602/139‑1 (MQ=255)  aGTCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGaa                                                                                                                           >  1:158661/1‑139 (MQ=255)   gTCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAAc                                                                                                                          >  1:91623/1‑139 (MQ=255)   gTCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAAc                                                                                                                          >  1:1386885/1‑139 (MQ=255)    tCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACa                                                                                                                         >  1:1638049/1‑139 (MQ=255)             gTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGTCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACctg                                                                                                                >  2:1377216/1‑139 (MQ=255)             gTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACACGATACctg                                                                                                                >  1:1002375/1‑139 (MQ=255)               gCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAAc                                                                                                                          >  2:813792/1‑127 (MQ=255)               gCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAAc                                                                                                                          <  1:813792/127‑1 (MQ=255)                 cAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCtgctgc                                                                                                            >  1:272706/1‑139 (MQ=255)                  aGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCg                                                                                                           >  1:480301/1‑139 (MQ=255)                             gagaGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTggcggc                                                                                                >  2:1336188/1‑139 (MQ=255)                              agagCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCa                                                                                               >  2:82785/1‑139 (MQ=255)                                agCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTg                                                                                                                                >  1:940629/1‑104 (MQ=255)                                agCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTg                                                                                                                                <  2:940629/104‑1 (MQ=255)                                agCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACg                                                                                             >  2:935583/1‑139 (MQ=255)                                    gtgtATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTtct                                                                                         <  1:82785/139‑1 (MQ=255)                                     tgtATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGtt                                                                                           >  2:464374/1‑136 (MQ=255)                                     tgtATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGtt                                                                                           <  1:464374/136‑1 (MQ=255)                                             ccATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGTCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCaaaa                                                                                <  1:1377216/139‑1 (MQ=255)                                             ccATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACCAGCCACTTACGCTGCTGAAAAATGTGAGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCATGTTCTCGGTCaaaa                                                                                <  2:1002375/139‑1 (MQ=255)                                               aTCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTggcggc                                                                                                >  1:578894/1‑121 (MQ=255)                                               aTCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTggcggc                                                                                                <  2:578894/121‑1 (MQ=255)                                               aTCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTgg                                                                                                    <  2:1614920/117‑1 (MQ=255)                                               aTCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTgg                                                                                                    >  1:1614920/1‑117 (MQ=255)                                               aTCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATg                                                                              >  1:877030/1‑139 (MQ=255)                                               aTCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATg                                                                              >  2:959751/1‑139 (MQ=255)                                               aTCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATg                                                                              >  1:1591464/1‑139 (MQ=255)                                                tCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATgg                                                                             <  1:1336188/139‑1 (MQ=255)                                                tCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATgg                                                                             <  2:158661/139‑1 (MQ=255)                                                tCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATgg                                                                             <  2:91623/139‑1 (MQ=255)                                                          tGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTg                                                                                                     >  1:972378/1‑105 (MQ=255)                                                          tGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTg                                                                                                     <  2:972378/105‑1 (MQ=255)                                                           ggCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATcc                                                                  >  2:588610/1‑139 (MQ=255)                                                            gCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCg                                                                 >  2:814367/1‑139 (MQ=255)                                                            gCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCGGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTAAAAATGGCCGAAATATCCg                                                                 <  1:841188/139‑1 (MQ=255)                                                              ccGTAAGCTGGGAGTGAAGGACGAGCCACTTACCATGCTGAAAAAGGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAAGAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATTTCCGGc                                                               >  1:902916/1‑139 (MQ=255)                                                                 tAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCgag                                                            >  1:899364/1‑139 (MQ=255)                                                                 tAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCgag                                                            >  2:860985/1‑139 (MQ=255)                                                                  aaGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCgaga                                                           <  2:1513096/139‑1 (MQ=255)                                                                              aaGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAAc                                                                                                                          >  1:356767/1‑64 (MQ=255)                                                                              aaGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAAc                                                                                                                          <  2:356767/64‑1 (MQ=255)                                                                              aaGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTgaaagaaa                                               >  1:811808/1‑139 (MQ=255)                                                                              aaGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTgaaagaaa                                               >  1:1441223/1‑139 (MQ=255)                                                                               aGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGaaaa                                              <  1:959751/139‑1 (MQ=255)                                                                                    gAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTg                                                                                                                             <  2:1177127/55‑1 (MQ=255)                                                                                    gAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTg                                                                                                                             >  1:1177127/1‑55 (MQ=255)                                                                                     aGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTgc                                        <  2:1190856/139‑1 (MQ=255)                                                                                     aGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTgc                                        >  1:1190856/1‑139 (MQ=255)                                                                                      gCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCg                                                                 >  2:1104654/1‑113 (MQ=255)                                                                                      gCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCg                                                                 <  1:1104654/113‑1 (MQ=255)                                                                                      gCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTgcg                                       >  2:325096/1‑139 (MQ=255)                                                                                      gCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGAGACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTgcg                                       <  1:1009598/139‑1 (MQ=255)                                                                                        cACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCa                                     <  2:1638049/139‑1 (MQ=255)                                                                                           ttACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGCTGGTGAAAGAAAAGGTTGCGCACCt                                  >  1:880428/1‑139 (MQ=255)                                                                                                   ctgAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATg                              >  2:125170/1‑135 (MQ=255)                                                                                                   ctgAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATg                              <  1:125170/135‑1 (MQ=255)                                                                                                    tgAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGt                         >  1:1003825/1‑139 (MQ=255)                                                                                                    tgAAAAATGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGt                         >  2:872328/1‑139 (MQ=255)                                                                                                         aaTGTGCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGcc                    <  2:169374/139‑1 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  <  2:1386885/139‑1 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  <  1:872328/139‑1 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  <  2:81448/139‑1 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  <  1:588610/139‑1 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  <  2:811808/139‑1 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  >  1:222001/1‑139 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  <  2:480301/139‑1 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  <  2:435980/139‑1 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  <  2:272706/139‑1 (MQ=255)                                                                                                           tgtgCGTGGACTGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTGGCGCACCTGATGGAAGTCCGCCCt                  >  2:920559/1‑139 (MQ=255)                                                                                                                      tGGAGCTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAAt       <  2:1591464/139‑1 (MQ=255)                                                                                                                           cTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTg  <  2:899364/139‑1 (MQ=255)                                                                                                                           cTGTTGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTg  <  1:325096/139‑1 (MQ=255)                                                                                                                              ttGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTgcg                                       >  1:26581/1‑99 (MQ=255)                                                                                                                              ttGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTgcg                                       <  2:26581/99‑1 (MQ=255)                                                                                                                              ttGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  <  2:608210/120‑1 (MQ=255)                                                                                                                              ttGATCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCt                  >  1:608210/1‑120 (MQ=255)                                                                                                                                  |                                                                                                                                    TAGTCGATGTAGGTGCCAGTTTGCAAGGGAGAGCGGTGTATCACCCATCTTGTAGCCTGGCCCGTAAGCTGGGAGTGAAGGACGAGCCACTTACGCTGCTGAAAAATGTGCGTGGACTGGAGCTGTTGACCTTTGCTGAACAGGATACCTGCTGCGGATTTGGCGGCACGTTCTCGGTCAAAATGGCCGAAATATCCGGCGAGATGGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTG  >  NC_000913/321969‑322229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑