| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,830,989 | T→C | V178A (GTT→GCT) | yicH → | putative inner membrane‑anchored periplasmic AsmA family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,830,989 | 0 | T | C | 88.0% | 139.4 / 8.6 | 50 | V178A (GTT→GCT) | yicH | putative inner membrane‑anchored periplasmic AsmA family protein |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base C (20/24); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01 | |||||||||||
GCCCGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGACGGCAGCTGGC > NC_000913/3830854‑3831117 | gCCCGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTcc > 2:746162/1‑139 (MQ=255) cccGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCt < 1:1255401/139‑1 (MQ=255) cccGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCt > 1:893063/1‑139 (MQ=255) cGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc < 2:168059/139‑1 (MQ=255) aTAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGccac < 2:1296355/139‑1 (MQ=255) aGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGccacca < 2:368490/139‑1 (MQ=255) aTGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTa < 1:742874/139‑1 (MQ=255) ggAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGAACt > 2:864739/1‑139 (MQ=255) aCTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGAtt < 2:966142/139‑1 (MQ=255) aGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAg < 1:1544417/139‑1 (MQ=255) aGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAg > 1:395779/1‑139 (MQ=255) aGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAg > 1:123269/1‑139 (MQ=255) aGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGT‑AAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAgg > 1:871927/1‑139 (MQ=255) gcgcgcAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAgg < 2:864183/139‑1 (MQ=255) gcgcAGCGGGTAAATGGCGGTGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCa < 1:1611529/139‑1 (MQ=255) cgcAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAg > 2:1472070/1‑139 (MQ=255) cgcAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAg < 2:280740/139‑1 (MQ=255) cAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTa < 2:123269/139‑1 (MQ=255) cAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTa < 1:1271816/139‑1 (MQ=255) cAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGAACTGATTGAAGGCAGTa < 1:864739/139‑1 (MQ=255) aGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTAt > 2:484939/1‑139 (MQ=255) gcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc > 1:404961/1‑97 (MQ=255) gcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGc < 2:404961/97‑1 (MQ=255) gcgTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGt > 1:1330322/1‑139 (MQ=255) tCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAAcc < 1:784082/139‑1 (MQ=255) aCCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTAc < 1:1623455/92‑1 (MQ=255) aCCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTAc > 2:1623455/1‑92 (MQ=255) ggTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGa > 2:857283/1‑139 (MQ=255) tACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACa < 1:1472070/139‑1 (MQ=255) cGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACAtt > 1:222633/1‑139 (MQ=255) gAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAg > 1:1621767/1‑78 (MQ=255) gAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAg < 2:1621767/78‑1 (MQ=255) cgcAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGa < 1:160310/79‑1 (MQ=255) cgcAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGa > 2:160310/1‑79 (MQ=255) cgcAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCt > 1:489676/1‑47 (MQ=255) cgcAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCt < 2:489676/47‑1 (MQ=255) gcAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGa < 1:857283/139‑1 (MQ=255) cAGATTCGGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAgg < 2:1077246/68‑1 (MQ=255) cAGATTCGGTTTAGTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATGGAAgg > 1:1077246/1‑68 (MQ=255) cAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCTTGCCACCAATGTAc < 2:1204573/58‑1 (MQ=255) cAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTAc > 1:1204573/1‑58 (MQ=255) gTGCCGGATCGCTTTCGCTCAATGATGCTCCTGccacc < 2:1503240/38‑1 (MQ=255) gTGCCGGATCGCTTTCGCTCAATGATGCTCCTGccacc > 1:1503240/1‑38 (MQ=255) gTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTa > 2:554891/1‑60 (MQ=255) gTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTa < 1:554891/60‑1 (MQ=255) gTGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTaa > 2:146396/1‑139 (MQ=255) tGCCGGATCGCTTTCGCTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAAc > 1:1528018/1‑139 (MQ=255) cTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCcgc < 2:399450/93‑1 (MQ=255) cTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCcgc > 1:399450/1‑93 (MQ=255) cTCAATGATGCTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGACGGCAGCTGGc < 2:53025/139‑1 (MQ=255) | GCCCGAATAGCGAATGGAAACTGAGCGCGCAGCGGGTAAATGGCGGCGTAGTTCCGTGGTCACCAAAAGCCGGTAAAGTGCTGGGTACGAAGGCGCAGATTCAGTTTAGTGCCGGATCGCTTTCGCTCAATGATGTTCCTGCCACCAATGTACTGATTGAAGGCAGTATTGATAACGATCGCGTTACGCTGACTAACCTGGGTGCCGACATCGCCCGCGGGACATTAACCGGAAACGCGCAGCGTAACGCCGACGGCAGCTGGC > NC_000913/3830854‑3831117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |