Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	337,217	T→C	V147A (GTC→GCC)	yahF →	putative NAD(P)‑binding succinyl‑CoA synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	337,217	0	T	C	100.0%	240.4 / NA	64	V147A (GTC→GCC)	yahF	putative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/33);  total (31/33)

CCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAT  >  NC_000913/337082‑337352                                                                                                                                        |                                                                                                                                        ccACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcctc                                                                                                                                      <  2:734415/139‑1 (MQ=255) ccACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcctc                                                                                                                                      <  1:1309305/139‑1 (MQ=255)       ttGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGa                                                                                                                                <  2:422731/139‑1 (MQ=255)       ttGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGa                                                                                                                                >  2:455321/1‑139 (MQ=255)       ttGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGa                                                                                                                                >  1:1625834/1‑139 (MQ=255)          gCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGa                                                                                                                             >  1:1359940/1‑139 (MQ=255)            aGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATg                                                                                                                           >  2:1344808/1‑139 (MQ=255)             gcgcCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGa                                                                                                                          >  1:647335/1‑139 (MQ=255)                           atCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGc                                                                                                            >  1:1477685/1‑139 (MQ=255)                                  aaaGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGc                                                                                                     >  2:776240/1‑139 (MQ=255)                                             gCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGaaaa                                                                                            >  2:112278/1‑137 (MQ=255)                                             gCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGaaaa                                                                                            <  1:112278/137‑1 (MQ=255)                                             gCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAg                                                                                          <  1:455321/139‑1 (MQ=255)                                             gCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAg                                                                                          <  2:1012279/139‑1 (MQ=255)                                              cGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcct                                                                                                                                       >  1:1558278/1‑93 (MQ=255)                                              cGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAgg                                                                                         >  2:1249242/1‑139 (MQ=255)                                               ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  2:1477685/139‑1 (MQ=255)                                               ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  1:907624/139‑1 (MQ=255)                                               ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  2:575712/139‑1 (MQ=255)                                               ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  1:790155/139‑1 (MQ=255)                                               ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  2:1183680/139‑1 (MQ=255)                                               ggTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  2:748413/139‑1 (MQ=255)                                                gTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGgc                                                                                       >  2:649063/1‑139 (MQ=255)                                                     ttCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGctgctg                                                                                  <  1:1225592/139‑1 (MQ=255)                                                         gTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatga                                                                              <  1:1344808/139‑1 (MQ=255)                                                            aaCGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATgg                                                                           >  2:620692/1‑139 (MQ=255)                                                               ggTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGc                                                                        <  1:167567/139‑1 (MQ=255)                                                                        gCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGcc                                                                                                    >  1:864257/1‑102 (MQ=255)                                                                        gCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGcc                                                                                                    <  2:864257/102‑1 (MQ=255)                                                                        gCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACtgtg                                                               >  1:1388262/1‑139 (MQ=255)                                                                           gCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  2:309770/111‑1 (MQ=255)                                                                           gCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        >  1:309770/1‑111 (MQ=255)                                                                           gCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCa                                                            <  1:776240/139‑1 (MQ=255)                                                                           gCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCa                                                            <  2:461826/139‑1 (MQ=255)                                                                             tCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcct                                                                                                                                       <  1:571019/62‑1 (MQ=255)                                                                             tCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGcct                                                                                                                                       >  2:571019/1‑62 (MQ=255)                                                                              cgcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACgg                                                         <  2:529136/139‑1 (MQ=255)                                                                              cgcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGAGGCACgg                                                         <  2:184169/139‑1 (MQ=255)                                                                               gcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCa                                                                                                  >  2:736324/1‑97 (MQ=255)                                                                               gcgAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCa                                                                                                  <  1:736324/97‑1 (MQ=255)                                                                                       cgTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCa                                                >  2:1240625/1‑139 (MQ=255)                                                                                               gcTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGt                                                                                                                                   <  2:78812/48‑1 (MQ=255)                                                                                               gcTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGt                                                                                                                                   >  1:78812/1‑48 (MQ=255)                                                                                                  gCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAAcggcgcggcg                                     <  1:649063/139‑1 (MQ=255)                                                                                                   cAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGtt                                                                                                                                  <  2:1228338/45‑1 (MQ=255)                                                                                                   cAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGCCTCAGtt                                                                                                                                  >  1:1228338/1‑45 (MQ=255)                                                                                                             tcAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        >  1:1082979/1‑77 (MQ=255)                                                                                                             tcAACGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAggg                                                                                        <  2:1082979/77‑1 (MQ=255)                                                                                                               aaCGTGATGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTg                                                                                                       >  2:981328/1‑60 (MQ=255)                                                                                                               aaCGTGATGCTGTTTTCCGATAACGCCGCAGTAGAAGATGAACTGGCGCTCAAGCAACTg                                                                                                       <  1:981328/60‑1 (MQ=255)                                                                                                                     aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCATGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCg                  >  2:540465/1‑139 (MQ=255)                                                                                                                     aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatga                                                                              >  1:1437822/1‑79 (MQ=255)                                                                                                                     aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCtgatga                                                                              <  2:1437822/79‑1 (MQ=255)                                                                                                                     aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACgg                                                         >  2:1328558/1‑100 (MQ=255)                                                                                                                     aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACgg                                                         <  1:1328558/100‑1 (MQ=255)                                                                                                                     aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCg                  >  2:1399659/1‑139 (MQ=255)                                                                                                                     aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCg                  >  1:85030/1‑139 (MQ=255)                                                                                                                     aTGCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGAGCTCAAGCAACTGGc                                                                                                     <  1:1004117/56‑1 (MQ=255)                                                                                                                     aTGCTGTTTTCCGACAACGCCTCAGTTGAAGATGAACTGGAGCTCAAGCAACTGGc                                                                                                     >  2:1004117/1‑56 (MQ=255)                                                                                                                       gCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGctgctg                                                                                  <  2:62326/73‑1 (MQ=255)                                                                                                                       gCTGTTTTCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGctgctg                                                                                  >  1:62326/1‑73 (MQ=255)                                                                                                                            tttCCGATAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTcg           >  2:1218487/1‑139 (MQ=255)                                                                                                                                  aTAACGCCTCAGTTGAATATGAACTGGCGCTCAAGCAACTGGCCCACGAATAAGGGCTGCTGGTGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa     <  1:1336183/139‑1 (MQ=255)                                                                                                                                  aTAACGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCaa     <  2:1359940/139‑1 (MQ=255)                                                                                                                                    aaCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACa   <  1:82572/139‑1 (MQ=255)                                                                                                                                    aaCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACa   <  2:1388262/139‑1 (MQ=255)                                                                                                                                     aCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACgg                                                         >  1:906467/1‑84 (MQ=255)                                                                                                                                     aCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACggcgcggcgc                                    <  2:564402/105‑1 (MQ=255)                                                                                                                                     aCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACggcgcggcgc                                    >  1:564402/1‑105 (MQ=255)                                                                                                                                     aCGCCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAt  >  2:570300/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        CCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGTAACCTGGCGGTGATTTCGGTCAACGGTCTGTTTGCCGCTCGCGAAGCGCGTCAGGCGCTGCAAAACGATCTCAACGTGATGCTGTTTTCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACAT  >  NC_000913/337082‑337352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑