| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 678,376 | C→T | P321L (CCG→CTG) | djlB → | putative HscC co‑chaperone, uncharacterized J domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 678,376 | 0 | C | T | 89.3% | 149.5 / 9.9 | 56 | P321L (CCG→CTG) | djlB | putative HscC co‑chaperone, uncharacterized J domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base T (28/22); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
ACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGTAAGCCGCG > NC_000913/678241‑678505 | actactGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCt < 2:690497/139‑1 (MQ=255) aGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTccc > 2:930648/1‑139 (MQ=255) tATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCg > 1:589910/1‑139 (MQ=255) tATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCg > 1:365635/1‑139 (MQ=255) tCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACa < 1:1498999/139‑1 (MQ=255) aCCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAgcg > 2:337733/1‑139 (MQ=255) cccAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAgcgc > 1:514016/1‑139 (MQ=255) ttGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 2:589910/139‑1 (MQ=255) ttGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 2:365635/139‑1 (MQ=255) gCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCAttt < 2:885262/135‑1 (MQ=255) gCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCAttt > 1:885262/1‑135 (MQ=255) cAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACg > 1:5047/1‑139 (MQ=255) gTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACg < 2:810950/134‑1 (MQ=255) gTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACg > 1:810950/1‑134 (MQ=255) gTGCCATCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATa > 1:1495390/1‑139 (MQ=255) gCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACt < 2:160590/139‑1 (MQ=255) ccGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTc > 2:303007/1‑139 (MQ=255) ccGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTc > 2:426170/1‑139 (MQ=255) cAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCg < 1:950443/139‑1 (MQ=255) cGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 1:51896/118‑1 (MQ=255) cGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat > 2:51896/1‑118 (MQ=255) gATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAActct < 1:328073/139‑1 (MQ=255) gATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAActct < 2:420/139‑1 (MQ=255) ttattaCCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAActctct < 2:1489191/139‑1 (MQ=255) cATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat > 2:333263/1‑102 (MQ=255) cATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 1:333263/102‑1 (MQ=255) aTTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTgggg > 2:1466669/1‑139 (MQ=255) ttACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGc > 1:694795/1‑139 (MQ=255) tACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCg > 2:1271525/1‑139 (MQ=255) gTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCAc > 2:1148019/1‑139 (MQ=255) tgatgatgaTTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat > 1:1484581/1‑93 (MQ=255) tgatgatgaTTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTtat < 2:1484581/93‑1 (MQ=255) atgatgatTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTa > 1:1319850/1‑76 (MQ=255) atgatgatTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTa < 2:1319850/76‑1 (MQ=255) gatgatTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTc > 2:1570062/1‑139 (MQ=255) atTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCgaga < 2:1495390/139‑1 (MQ=255) aaGCGCGTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGt > 1:236289/1‑139 (MQ=255) cgTGATCTGGTTAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGc > 2:1457009/1‑139 (MQ=255) ggTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCg < 2:126631/38‑1 (MQ=255) ggTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCg > 1:126631/1‑38 (MQ=255) ttAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGtt > 2:72923/1‑57 (MQ=255) ttAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGtt < 1:72923/57‑1 (MQ=255) ttAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACg < 1:261563/139‑1 (MQ=255) tAACGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGt > 1:179573/1‑139 (MQ=255) cGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGa < 2:1574235/36‑1 (MQ=255) cGATCAGGGAAAACTGCTACTTTATGTCCCCGGCGa > 1:1574235/1‑36 (MQ=255) tCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTg > 1:684152/1‑106 (MQ=255) tCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTg < 2:684152/106‑1 (MQ=255) tCAGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGTAAGCcg < 1:303007/139‑1 (MQ=255) aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTAcg > 1:876836/1‑44 (MQ=255) aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTAcg < 2:876836/44‑1 (MQ=255) aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCAttt > 1:1348914/1‑55 (MQ=255) aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCAttt < 2:1348914/55‑1 (MQ=255) aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTta < 1:765644/56‑1 (MQ=255) aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTta > 2:765644/1‑56 (MQ=255) aGGGAAAACTGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGAGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGTAAGCcgcg > 2:929186/1‑139 (MQ=255) | ACTACTGGCGCGAGTATCCCTCCACCCAGGCGGATGCTTTAATGTTGCAATGGTGCCGTCAGCATCGGGTCGATTATTACCCATTACTGGTGATGATGATTGAAGCGCGTGATCTGGTTAACGATCAGGGAAAACCGCTACTTTATGTCCCCGGCGACAGCGCCCGTACGCGTTTTCATTTATACGAAATACTCAGCGATGAAAAACTCTCTGCGCTGGGGCGTTCACTGGTCGAGATGGTTTTGCACAAAGGACGTAAGCCGCG > NC_000913/678241‑678505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |