Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,361,052	(A)6→7	intergenic (‑144/‑68)	puuA ← / → puuD	glutamate‑‑putrescine ligase/gamma‑glutamyl‑gamma‑aminobutyrate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,361,046	1	.	A	100.0%	179.5 / NA	53	intergenic (‑138/‑74)	puuA/puuD	glutamate‑‑putrescine ligase/gamma‑glutamyl‑gamma‑aminobutyrate hydrolase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (30/23);  total (30/23)

TGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTG‑AAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGC  >  NC_000913/1360922‑1361174                                                                                                                              |                                                                                                                                 tGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGaa                                                                                                                     <  1:826680/139‑1 (MQ=255)                          aGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTa                                                                                            <  2:456506/139‑1 (MQ=255)                            cGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAAt                                                                                          >  1:101068/1‑139 (MQ=255)                              tAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTG‑aaaaaaatat                                                                                                                        <  2:540831/106‑5 (MQ=255)                              tAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTG‑aaaaaaatat                                                                                                                        >  1:540831/1‑102 (MQ=255)                                aaaTATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCg                                                                                      <  2:256234/139‑1 (MQ=255)                                aaaTATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCg                                                                                      <  2:1079354/139‑1 (MQ=255)                                        tGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAATATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTa                                                                              >  1:46314/1‑139 (MQ=255)                                           ccaccaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTc                                                                           <  1:16129/139‑1 (MQ=255)                                           ccaccaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTc                                                                           >  1:736065/1‑139 (MQ=255)                                             accaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGa                                                                                     <  1:183185/127‑1 (MQ=255)                                             accaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGa                                                                                     >  2:183185/1‑127 (MQ=255)                                             accaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         >  1:101629/1‑139 (MQ=255)                                             accaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         >  2:448703/1‑139 (MQ=255)                                             accaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         <  1:827692/139‑1 (MQ=255)                                             accaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         >  2:762398/1‑139 (MQ=255)                                             accaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         >  2:909899/1‑139 (MQ=255)                                             accaTTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         >  2:921649/1‑139 (MQ=255)                                                 ttCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACttt                                                                     >  1:268369/1‑139 (MQ=255)                                                       tCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTgaga                                                               <  1:416447/139‑1 (MQ=255)                                                         tGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         >  1:78058/1‑127 (MQ=255)                                                         tGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         <  2:78058/127‑1 (MQ=255)                                                         tGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGAtt                                                             >  1:655322/1‑139 (MQ=255)                                                            aTGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTG‑aaaaaaata                                                                                                                         >  1:128390/1‑72 (MQ=255)                                                            aTGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTG‑aaaaaaata                                                                                                                         <  2:128390/75‑4 (MQ=255)                                                               taaaCTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCATTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCAt                                                       <  1:559372/138‑1 (MQ=255)                                                               cAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCAt                                                       <  2:736065/139‑1 (MQ=255)                                                                  aCTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGa                                                    >  1:928191/1‑139 (MQ=255)                                                                  aCTAAATGTTTGTAAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGa                                                    <  2:46314/139‑1 (MQ=255)                                                                    tAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGaaa                                                  >  2:947891/1‑139 (MQ=255)                                                                     aaaTGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGaaaa                                                 >  2:281301/1‑139 (MQ=255)                                                                     aaaTGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGaaa                                                  >  1:736745/1‑138 (MQ=255)                                                                     aaaTGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGaaa                                                  <  2:736745/138‑1 (MQ=255)                                                                           ttGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAAt                                           <  2:928191/139‑1 (MQ=255)                                                                            tGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         <  1:29110/108‑1 (MQ=255)                                                                            tGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         >  2:29110/1‑108 (MQ=255)                                                                            tGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATg                                          >  2:567004/1‑139 (MQ=255)                                                                              tCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGa                                         <  2:488728/138‑1 (MQ=255)                                                                              tCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGa                                         >  1:488728/1‑138 (MQ=255)                                                                              tCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAAAGGCGACAATGTCGTCGACATTGTGAGATTGAGCATGGAAAATATAATGaa                                        >  2:409459/1‑139 (MQ=255)                                                                                  aTGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTG‑aaaaaaatatt                                                                                                                       >  2:96770/1‑50 (MQ=255)                                                                                  aTGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTG‑aaaaaaatatt                                                                                                                       <  1:96770/55‑6 (MQ=255)                                                                                          ttgtgcttGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTAt                            <  1:762398/133‑1 (MQ=255)                                                                                          ttGAGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTAt                            <  1:448703/139‑1 (MQ=255)                                                                                             aGTTTGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCgg                         >  1:314521/1‑139 (MQ=255)                                                                                               tttGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         <  2:486825/89‑1 (MQ=255)                                                                                               tttGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCa                                                                         >  1:486825/1‑89 (MQ=255)                                                                                               tttGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCGgtg                       >  1:898511/1‑139 (MQ=255)                                                                                               tttGCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCGgtg                       >  1:73576/1‑139 (MQ=255)                                                                                                  gCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAGTATAATGAAc                                       >  1:486538/1‑120 (MQ=255)                                                                                                  gCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAAc                                       <  2:486538/120‑1 (MQ=255)                                                                                                  gCAAAAATGAAAACCCACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCg                    >  1:954547/1‑139 (MQ=255)                                                                                                                ccACTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAAc      <  2:73576/139‑1 (MQ=255)                                                                                                                  aCTGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAg    >  1:463317/1‑139 (MQ=255)                                                                                                                    tGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCg                          <  2:504469/115‑1 (MQ=255)                                                                                                                    tGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCg                          >  1:504469/1‑115 (MQ=255)                                                                                                                    tGCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGc  >  2:339991/1‑139 (MQ=255)                                                                                                                     gCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGAtt                                                             <  2:572254/79‑1 (MQ=255)                                                                                                                     gCTAGATTGAAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGAtt                                                             >  1:572254/1‑79 (MQ=255)                                                                                                                              |                                                                                                                                 TGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACCCACTGCTAGATTG‑AAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATGGCGATAATTTAGTCCACTTTGTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGC  >  NC_000913/1360922‑1361174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑