Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,734,543 (C)8→9 coding (492/732 nt) yfiH ← UPF0124 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,734,5351.C100.0% 74.6 / NA 21E167G (GAG→GGG) yfiHUPF0124 family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (12/9);  total (12/9)

TTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCA  >  NC_000913/2734408‑2734660
                                                                                                                                |                                                                                                                             
ttGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTcccccc                                                                                                                         >  1:1109926/1‑134 (MQ=255)
ttGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTcccccc                                                                                                                         <  2:1109926/134‑1 (MQ=255)
                                 ggCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTaa                                                                                    <  1:306682/139‑1 (MQ=255)
                                                               aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTcccccc                                                                                                                         <  1:1063315/71‑1 (MQ=255)
                                                               aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTcccccc                                                                                                                         >  2:1063315/1‑71 (MQ=255)
                                                                      tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                             >  2:69756/1‑93 (MQ=255)
                                                                      tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                             <  1:69756/93‑1 (MQ=255)
                                                                      tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACa                                                >  1:1074898/1‑138 (MQ=255)
                                                                      tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACa                                                <  2:1074898/138‑1 (MQ=255)
                                                                      tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg                                               >  1:854488/1‑139 (MQ=255)
                                                                         tgaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc                                                                                        >  1:37468/1‑95 (MQ=255)
                                                                         tgaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGcc                                                                                        <  2:37468/95‑1 (MQ=255)
                                                                           aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                             <  2:82130/88‑1 (MQ=255)
                                                                           aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                             >  1:82130/1‑88 (MQ=255)
                                                                                  gCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAAAGGAAACCGTctct                                   >  1:79109/1‑139 (MQ=255)
                                                                                     gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGaa                                                                                                               <  1:1049005/60‑1 (MQ=255)
                                                                                     gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGaa                                                                                                               >  2:1049005/1‑60 (MQ=255)
                                                                                      cACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCa                               >  2:756334/1‑139 (MQ=255)
                                                                                                aGCGTCTACTGCCATAAACGCCTCGCGAACCTcccccccc                                                                                                                       >  1:523375/1‑40 (MQ=255)
                                                                                                aGCGTCTACTGCAATAAACGCCTCGCGAACCTcccccccc                                                                                                                       <  2:523375/40‑1 (MQ=255)
                                                                                                   gTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                  <  1:1012795/139‑1 (MQ=255)
                                                                                                      tACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGaa                                                                                                               <  1:51323/43‑1 (MQ=255)
                                                                                                      tACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGaa                                                                                                               >  2:51323/1‑43 (MQ=255)
                                                                                                      tACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                             <  1:941506/61‑1 (MQ=255)
                                                                                                      tACTGCCATAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                             >  2:941506/1‑61 (MQ=255)
                                                                                                              tAAACGCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgcc       >  1:203431/1‑139 (MQ=255)
                                                                                                                   gCCTCGCGAACCTCCCCCCCCCACTTCGAACGCGCGCGGACCAATTGCAGGCCCTAACCAGGCGAGAATATTTTCCGGATCATCAGCAAAACAGGAAACCGACGCTTCCGGCCAGCCTGCGCACAGTCAACgccatcca  >  1:724485/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                             
TTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAACCTCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCA  >  NC_000913/2734408‑2734660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: