Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	683,121	C→T	A91A (GCG→GCA)	hscC ←	Hsp70 family chaperone Hsc62, RpoD‑binding transcription inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	683,121	0	C	T	100.0%	190.0 / NA	55	A91A (GCG→GCA)	hscC	Hsp70 family chaperone Hsc62, RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/24);  total (31/24)

CTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGCGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCGGTTTTCCGACTAAAATATGATTAT  >  NC_000913/682985‑683251                                                                                                                                         |                                                                                                                                   ctgctAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGgtgc                                                                                                                                  >  1:1062203/1‑139 (MQ=255) ctgctAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGgtgc                                                                                                                                  >  2:147014/1‑139 (MQ=255)        aCGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAAt                                                                                                                           <  1:169700/139‑1 (MQ=255)                      gttgttCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTaa                                                                                                             >  1:1041347/1‑139 (MQ=255)                           tCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGcc                                                                                                        >  2:895100/1‑139 (MQ=255)                                 cTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTgg                                                                                                  >  1:496329/1‑139 (MQ=255)                                  tGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGt                                                                                                 >  1:783538/1‑139 (MQ=255)                                           ccGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGcc                                                                                        <  2:1062122/139‑1 (MQ=255)                                                aCGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTg                                                                                   <  1:370944/139‑1 (MQ=255)                                                aCGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTg                                                                                   <  1:895100/139‑1 (MQ=255)                                                aCGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTg                                                                                   <  2:95636/139‑1 (MQ=255)                                                aCGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTg                                                                                   <  2:783147/139‑1 (MQ=255)                                                         accacATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTaa                                                                          >  1:1078017/1‑139 (MQ=255)                                                              aTCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGCGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAGTAAc                                                                     >  2:739359/1‑139 (MQ=255)                                                               tCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACg                                                                    <  2:453468/139‑1 (MQ=255)                                                                cTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCGCATTGCACGTTTAAATAACGc                                                                   >  2:659479/1‑139 (MQ=255)                                                                cTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGc                                                                   >  1:611012/1‑139 (MQ=255)                                                                  ttAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAATGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTg                                                                 >  1:292340/1‑139 (MQ=255)                                                                  ttAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTg                                                                 >  2:711432/1‑139 (MQ=255)                                                                  ttAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTg                                                                 >  1:1015291/1‑139 (MQ=255)                                                                   tAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGc                                                                >  2:263214/1‑139 (MQ=255)                                                                     aTCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCg                                                              >  1:568759/1‑139 (MQ=255)                                                                          aCGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGt                                                                                                     >  2:202803/1‑95 (MQ=255)                                                                          aCGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGt                                                                                                     >  1:713044/1‑95 (MQ=255)                                                                          aCGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGt                                                                                                     <  1:202803/95‑1 (MQ=255)                                                                          aCGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGt                                                                                                     <  2:713044/95‑1 (MQ=255)                                                                          aCGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGcc                                                                                        <  1:696309/108‑1 (MQ=255)                                                                          aCGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGcc                                                                                        >  2:696309/1‑108 (MQ=255)                                                                          aCGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTa                                                         >  1:393347/1‑139 (MQ=255)                                                                            gTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCACTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATc                                                       <  1:800296/139‑1 (MQ=255)                                                                               gCAGAAATTGTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAAGAACGCTGCCGTTTTATCCgg                                                    <  1:659479/139‑1 (MQ=255)                                                                                       tcttcGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAg                                            <  1:370912/139‑1 (MQ=255)                                                                                       tcttcGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAg                                            <  2:611012/139‑1 (MQ=255)                                                                                                 cttcttTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGa                                  >  1:455242/1‑139 (MQ=255)                                                                                                 cttcttTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGa                                  <  2:393347/139‑1 (MQ=255)                                                                                                           tGAGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCGGt                        >  1:221504/1‑139 (MQ=255)                                                                                                             aGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCg                          >  2:519216/1‑135 (MQ=255)                                                                                                             aGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCg                          <  1:519216/135‑1 (MQ=255)                                                                                                             aGCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCGGttt                      <  2:1078017/139‑1 (MQ=255)                                                                                                              gCGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCGGtttt                     >  2:859771/1‑139 (MQ=255)                                                                                                               cGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCa                                                                                                       <  2:288047/56‑1 (MQ=255)                                                                                                               cGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCa                                                                                                       >  2:798025/1‑56 (MQ=255)                                                                                                               cGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCa                                                                                                       <  1:798025/56‑1 (MQ=255)                                                                                                               cGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCa                                                                                                       >  1:263693/1‑56 (MQ=255)                                                                                                               cGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCa                                                                                                       >  1:288047/1‑56 (MQ=255)                                                                                                               cGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCa                                                                                                       <  2:263693/56‑1 (MQ=255)                                                                                                               cGTAATACCAAAGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCGGTTTTc                    >  2:368948/1‑139 (MQ=255)                                                                                                                   aTACCAAAGAGGACAGTTCTGGTGCGTTAAATgtgt                                                                                                                       <  2:1079122/36‑1 (MQ=255)                                                                                                                   aTACCAAAGAGGACAGTTCTGGTGCGTTAAATgtgt                                                                                                                       >  1:1079122/1‑36 (MQ=255)                                                                                                                         aaGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGaa                                             >  1:75968/1‑104 (MQ=255)                                                                                                                         aaGAGGACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGaa                                             <  2:75968/104‑1 (MQ=255)                                                                                                                              gACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTg                                                                                   >  2:261647/1‑61 (MQ=255)                                                                                                                              gACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTg                                                                                   <  1:261647/61‑1 (MQ=255)                                                                                                                              gACAGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCGGTTTTCCGACTAAAATATGat     >  1:1030757/1‑139 (MQ=255)                                                                                                                                 aGTTCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCGGTTTTCCGACTAAAATATGattat  >  2:286051/1‑139 (MQ=255)                                                                                                                                   ttCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATc                                                       <  2:19265/84‑1 (MQ=255)                                                                                                                                   ttCTGGTGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATc                                                       >  1:19265/1‑84 (MQ=255)                                                                                                                                         |                                                                                                                                   CTGCTAAACGGGTATGCTTGCGTTGTTCATCGCTGAAATAAGCCGGAACGGAGATCACCACATCTTTAATCGGACGTTGCAGAAATTCTTCGGCATCTTCTTTTAATGAGCGTAATACCAAAGAGGACAGTTCTGGCGCGTTAAATGTGTCGCTGCCTAACCGCCAGTTGGTATTACTGCCCATTGCACGTTTAAATAACGCTGCCGTTTTATCCGGATGCGAAGTACGCCGTGATACAGCCGGTTTTCCGACTAAAATATGATTAT  >  NC_000913/682985‑683251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑