Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	895,545	T→C	R185R (CGT→CGC)	potG →	putrescine ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	895,545	0	T	C	100.0%	170.5 / NA	46	R185R (CGT→CGC)	potG	putrescine ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/23);  total (23/23)

TTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTC  >  NC_000913/895414‑895677                                                                                                                                    |                                                                                                                                     ttCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGa                                                                                                                               >  2:207425/1‑139 (MQ=255)   cGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGGTg                                                                                                                             >  2:325258/1‑139 (MQ=255)   cGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATg                                                                                                                             >  1:916931/1‑139 (MQ=255)   cGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATg                                                                                                                             >  1:71998/1‑139 (MQ=255)    gCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGc                                                                                                                            >  2:1036660/1‑139 (MQ=255)          cGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTg                                                                                                                      <  2:71998/139‑1 (MQ=255)          cGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTg                                                                                                                      <  1:207425/139‑1 (MQ=255)                   cATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGtggtgg                                                                                                             <  1:1005587/139‑1 (MQ=255)                   cATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGtggtgg                                                                                                             <  1:384590/139‑1 (MQ=255)                    aTCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATT‑CTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGat                                                                                                           >  1:412139/1‑139 (MQ=255)                         cTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTc                                                                                                       >  2:258768/1‑139 (MQ=255)                             ccGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGa                                                                                                   <  1:49511/139‑1 (MQ=255)                                   gtCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGt                                                                                             <  2:949121/139‑1 (MQ=255)                                         gACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGgtgt                                                                                       <  2:392816/139‑1 (MQ=255)                                             aCGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACt                                                                                   >  1:87181/1‑139 (MQ=255)                                                      cctggcccGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATg                                                                          >  2:1023528/1‑139 (MQ=255)                                                        tggcccGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGt                                                                        <  2:850450/139‑1 (MQ=255)                                                         ggcccGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTc                                                                       >  1:730700/1‑139 (MQ=255)                                                            ccGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCAcc                                                                    <  2:916931/139‑1 (MQ=255)                                                              gAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCa                                                                  <  2:87181/139‑1 (MQ=255)                                                              gAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCa                                                                  <  1:258768/139‑1 (MQ=255)                                                               aaGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGgtgt                                                                                       <  1:712530/117‑1 (MQ=255)                                                               aaGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGgtgt                                                                                       >  2:712530/1‑117 (MQ=255)                                                                     tGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAgtggt                                                                                                               >  2:327942/1‑87 (MQ=255)                                                                     tGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAgtggt                                                                                                               <  1:327942/87‑1 (MQ=255)                                                                            cgcCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCAc                                                                 >  2:923344/1‑126 (MQ=255)                                                                            cgcCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCAc                                                                 <  1:923344/126‑1 (MQ=255)                                                                               ccGAAACTATT‑CTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGAt                                                <  2:412139/139‑1 (MQ=255)                                                                                             gCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTg                                                                                                                      >  1:503221/1‑56 (MQ=255)                                                                                             gCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTg                                                                                                                      <  2:503221/56‑1 (MQ=255)                                                                                             gCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCAccc                                                                   <  2:184734/107‑1 (MQ=255)                                                                                             gCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCAccc                                                                   >  1:184734/1‑107 (MQ=255)                                                                                              cTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGgc                                  >  1:548247/1‑139 (MQ=255)                                                                                              cTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGgc                                  >  1:36020/1‑139 (MQ=255)                                                                                               tCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGgcg                                 >  2:380811/1‑139 (MQ=255)                                                                                                  aTGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCAt                              <  2:730700/139‑1 (MQ=255)                                                                                                  aTGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCAt                              <  2:1038163/139‑1 (MQ=255)                                                                                                    gAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCg                            <  2:467712/139‑1 (MQ=255)                                                                                                    gAGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCg                            <  2:270166/139‑1 (MQ=255)                                                                                                     aGCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGc                           >  2:675622/1‑139 (MQ=255)                                                                                                      gCCGATGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGcc                          >  1:960988/1‑139 (MQ=255)                                                                                                           tGGGCGCGCTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTAt                     >  2:443995/1‑139 (MQ=255)                                                                                                                 cgcTGGATAAAAAGCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCg               >  1:272542/1‑139 (MQ=255)                                                                                                                              gCTGCGCGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTc  <  1:1036660/139‑1 (MQ=255)                                                                                                                                tGCGCGACAGGACGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGa                                                               <  2:434695/76‑1 (MQ=255)                                                                                                                                tGCGCGACAGGACGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGa                                                               >  1:434695/1‑76 (MQ=255)                                                                                                                                    |                                                                                                                                     TTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGGATATTCTGGAGCGCGTCGGTGTGACTTGTGTGATGGTCACCCACGATCAGGAAGAGGCGATGACCATGGCGGGGCGCATCGCCATTATGAATCGTGGGAAATTTGTC  >  NC_000913/895414‑895677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑