Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	924,093	A→G	D277G (GAC→GGC)	clpA →	ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	924,093	0	A	G	100.0%	104.9 / NA	28	D277G (GAC→GGC)	clpA	ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/15);  total (13/15)

TTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAC  >  NC_000913/923955‑924228                                                                                                                                           |                                                                                                                                        ttGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGa                                                                                                                                         <  1:1072350/139‑1 (MQ=255)       aGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTaa                                                                                                                                   <  1:1102397/139‑1 (MQ=255)              tgtgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                            <  1:925042/139‑1 (MQ=255)              tgtgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATc                                                                                                                            <  1:524765/139‑1 (MQ=255)               gtgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATcc                                                                                                                           <  2:1000276/139‑1 (MQ=255)                tgCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCt                                                                                                                          <  1:418244/139‑1 (MQ=255)                     gAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTa                                                                                                                     <  1:427199/139‑1 (MQ=255)                           aTGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTGACAGCATCCTGTTTATtgatg                                                                                                               <  1:413782/139‑1 (MQ=255)                                 gACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATcc                                                                                                         <  1:25380/139‑1 (MQ=255)                                         gATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAGACGTTTTAAAGCGTTGCTCAAGCAGCTGAAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCAtt                                                                                                 >  2:666578/1‑139 (MQ=255)                                                   ctcGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCgg                                                                                       >  1:691925/1‑139 (MQ=255)                                                       atatCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGc                                                                                   >  2:1027531/1‑139 (MQ=255)                                                              ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGAt                                                                                                           <  2:203707/108‑1 (MQ=255)                                                              ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGAt                                                                                                           >  1:203707/1‑108 (MQ=255)                                                              ttCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCt                                                                            >  1:981658/1‑139 (MQ=255)                                                                ctctGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgatga                                                                                                              <  2:325487/103‑1 (MQ=255)                                                                ctctGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgatga                                                                                                              >  1:325487/1‑103 (MQ=255)                                                                          gggCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGgcac                                                                                                                                      <  2:66172/69‑1 (MQ=255)                                                                          gggCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGgcac                                                                                                                                      >  1:66172/1‑69 (MQ=255)                                                                           ggCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCg                                                               >  1:589206/1‑139 (MQ=255)                                                                                     atCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTaa                                                     <  2:981658/139‑1 (MQ=255)                                                                                            cgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgatg                                                                                                               <  2:1110067/74‑1 (MQ=255)                                                                                            cgACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATtgatg                                                                                                               >  1:1110067/1‑74 (MQ=255)                                                                                              aCTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCaa                                            >  1:291900/1‑139 (MQ=255)                                                                                                          gTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCtctc                                >  1:916224/1‑139 (MQ=255)                                                                                                             ttAAAGCGTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAg                             <  1:355063/139‑1 (MQ=255)                                                                                                                    gTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTaaa                      >  2:1100554/1‑139 (MQ=255)                                                                                                                    gTTGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCAAGCGGTaaa                      >  1:767711/1‑139 (MQ=255)                                                                                                                      tGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAAt                    <  1:669369/139‑1 (MQ=255)                                                                                                                      tGCTCAAGCAGCTGGAGCAGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAAt                    >  2:669369/1‑139 (MQ=255)                                                                                                                                        aGGGCACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAc  <  2:589206/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                        TTGTTCAGGGCGATGTGCCGGAAGTGATGGCTGACTGTACGATTTACTCTCTCGATATCGGTTCTCTGTTAGCGGGCACAAAATATCGCGGCGACTTTGAAAAACGTTTTAAAGCGTTGCTCAAGCAGCTGGAGCAGGACACTAACAGCATCCTGTTTATTGATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGAC  >  NC_000913/923955‑924228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑