Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,846,908	A→G	V18A (GTC→GCC)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,846,908	0	A	G	100.0%	81.4 / NA	24	V18A (GTC→GCC)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/9);  total (15/9)

CGCATAGCGGCTGTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGACATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCCGCGCGCTGGCACCAGTTCGCCAATT  >  NC_000913/1846784‑1847019                                                                                                                             |                                                                                                                cgcATAGCGGCTGTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTAGGCAGGGCATCAGCAATGgcg                                                                                                   >  2:657979/1‑139 (MQ=255)     atgCGGCTGACGTAGGCTTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCATGTCACCACCTGACAATTACCGCACCCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGGAATGgcgcgcg                                                                                               <  1:658537/137‑1 (MQ=255)             gTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCaa                                                                                                        >  1:179702/1‑122 (MQ=255)             gTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCaa                                                                                                        <  2:179702/122‑1 (MQ=255)                gTAGGCGTCTGGCTGCGCCTGCTCAAGCTGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGt                                                                                    >  2:694582/1‑139 (MQ=255)                    gCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGttttt                                                                                <  1:657979/139‑1 (MQ=255)                     cGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTc                                                                               <  1:289005/139‑1 (MQ=255)                        cTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGc                                                                            >  1:1077870/1‑139 (MQ=255)                                 ccTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAaacaa                                                                   <  1:295135/139‑1 (MQ=255)                                               tGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTaa                                                     >  1:1050523/1‑139 (MQ=255)                                                     aTACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAAtc                                               >  1:794582/1‑139 (MQ=255)                                                            aGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCa                                        >  1:69527/1‑139 (MQ=255)                                                                caccacCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGc                                    >  2:839624/1‑139 (MQ=255)                                                                 accaccTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCa                                   >  1:205139/1‑139 (MQ=255)                                                                   caccTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACg                                 >  1:1116416/1‑139 (MQ=255)                                                                              tACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCcgcgcg                      <  1:694582/139‑1 (MQ=255)                                                                                   cACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCAt                                       <  1:375670/117‑1 (MQ=255)                                                                                   cACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCAt                                       >  2:375670/1‑117 (MQ=255)                                                                                        gATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCCGCGCGCTGGCACCAg            >  2:355028/1‑139 (MQ=255)                                                                                                  tCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCCGCGCGCTGGCACCAGTTCGCCAAtt  >  1:442027/1‑139 (MQ=255)                                                                                                         tCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAAc                                          <  2:739541/92‑1 (MQ=255)                                                                                                         tCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGACAATAAACAACCGCATTGAATTAACGAATCTCAAc                                          >  1:739541/1‑92 (MQ=255)                                                                                                                cGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCa                                                              <  2:248588/65‑1 (MQ=255)                                                                                                                cGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCa                                                              >  1:248588/1‑65 (MQ=255)                                                                                                                             |                                                                                                                CGCATAGCGGCTGTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGACATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCCGCGCGCTGGCACCAGTTCGCCAATT  >  NC_000913/1846784‑1847019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑