Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,135,962	T→A	D46V (GAT→GTT)	wzb ←	colanic acid production protein‑tyrosine‑phosphatase, Wzc‑P dephosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,135,962	0	T	A	100.0%	150.6 / NA	43	D46V (GAT→GTT)	wzb	colanic acid production protein‑tyrosine‑phosphatase, Wzc‑P dephosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (22/21);  total (22/21)

GTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGATCAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGATG  >  NC_000913/2135834‑2136087                                                                                                                                 |                                                                                                                              gTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCtt                                                                                                                     >  2:107875/1‑139 (MQ=255)  ttCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTa                                                                                                                    >  2:764741/1‑139 (MQ=255)                       ggTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCa                                                                                               >  1:720243/1‑139 (MQ=255)                        gTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAg                                                                                              <  1:629851/139‑1 (MQ=255)                              aTCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACt                                                                                        <  1:548642/139‑1 (MQ=255)                                   gTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACt                                                                                   >  1:858596/1‑139 (MQ=255)                                    tCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACtt                                                                                  <  2:327003/139‑1 (MQ=255)                                       tAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCa                                                                               >  2:346746/1‑139 (MQ=255)                                        aGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAg                                                                              >  2:197193/1‑139 (MQ=255)                                          ttGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCt                                                                            <  2:494037/139‑1 (MQ=255)                                               acacAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCggg                                                                       <  2:840703/139‑1 (MQ=255)                                                    gACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATa                                                                  >  2:1026374/1‑139 (MQ=255)                                                    gACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATa                                                                  >  1:908117/1‑139 (MQ=255)                                                     aCGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACttt                                                                                 <  2:463978/123‑1 (MQ=255)                                                     aCGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACttt                                                                                 >  1:463978/1‑123 (MQ=255)                                                     aCGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATaa                                                                 >  2:888197/1‑139 (MQ=255)                                                                        gCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTccg                                              <  1:764741/139‑1 (MQ=255)                                                                        gCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTccg                                              <  2:720243/139‑1 (MQ=255)                                                                              tGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCg                                        >  2:607642/1‑139 (MQ=255)                                                                                       agagACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGc                               <  2:132612/139‑1 (MQ=255)                                                                                        gagaCAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCa                              >  2:356154/1‑139 (MQ=255)                                                                                          gaCAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCggg                                      <  1:286167/129‑1 (MQ=255)                                                                                          gaCAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCggg                                      >  2:286167/1‑129 (MQ=255)                                                                                               ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTc                                                                           <  1:694444/87‑1 (MQ=255)                                                                                               ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTc                                                                           >  2:694444/1‑87 (MQ=255)                                                                                               ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTg                       >  1:684515/1‑139 (MQ=255)                                                                                               ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTg                       >  1:2092/1‑139 (MQ=255)                                                                                               ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTg                       >  2:9921/1‑139 (MQ=255)                                                                                               ttGATGTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACACCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTg                       >  1:745530/1‑139 (MQ=255)                                                                                                    gTTCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGac                  <  1:607642/139‑1 (MQ=255)                                                                                                      tCTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGacac                <  1:197193/139‑1 (MQ=255)                                                                                                       cTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAat                           >  2:1027996/1‑127 (MQ=255)                                                                                                       cTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAat                           <  1:1027996/127‑1 (MQ=255)                                                                                                       cTGCGGCGACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGAcaca               <  2:147132/139‑1 (MQ=255)                                                                                                         gcggcgACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGa             <  2:60377/139‑1 (MQ=255)                                                                                                          cggcgACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGAc            >  2:466145/1‑139 (MQ=255)                                                                                                             cgACGCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAAc         >  1:251570/1‑139 (MQ=255)                                                                                                                 gCTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAg     <  1:657105/139‑1 (MQ=255)                                                                                                                  cTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGa    <  1:888197/139‑1 (MQ=255)                                                                                                                  cTGATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGa    <  2:908117/139‑1 (MQ=255)                                                                                                                    gATAGCGGTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGAtg  <  1:9921/139‑1 (MQ=255)                                                                                                                           gTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGAcaca               >  1:908362/1‑119 (MQ=255)                                                                                                                           gTAGGAACAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGAcaca               <  2:908362/119‑1 (MQ=255)                                                                                                                                 |                                                                                                                              GTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGTTGCGACACAGACGGCGGCTGATTTGACGGGCACAGTGACCTTCCAGAGACAGTTGATGTTCTGCGGCGACGCTGATAGCGGTAGGATCAGCGCCCTTACCGACCAGCGCGCCGAGTCCAGCGGACTCCACTTTCAGCTCCGGGTGATAACGTTGCAGTAAGCGTTCCGCCGTCGGGGAACGGCAAATATTGCCGACACAGACAACTAAGATG  >  NC_000913/2135834‑2136087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑