Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,215,997	A→G	R160R (CGT→CGC)	yehW ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,215,997	0	A	G	100.0%	184.5 / NA	49	R160R (CGT→CGC)	yehW	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/27);  total (22/27)

AAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTG  >  NC_000913/2215862‑2216130                                                                                                                                        |                                                                                                                                      aaGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTgcgc                                                                                                                                    <  1:1118805/139‑1 (MQ=255)                  gCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  2:138130/139‑1 (MQ=255)                  gCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  2:979655/139‑1 (MQ=255)                  gCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  2:820476/139‑1 (MQ=255)                  gCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  2:666923/139‑1 (MQ=255)                  gCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  1:448679/139‑1 (MQ=255)                    gTACCCATCGGGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAAGGTTGATAATCACCGAGGGTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACGCa                                                                                                                 <  1:190517/139‑1 (MQ=255)                          aGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCa                                                                                                           >  2:796258/1‑139 (MQ=255)                                cTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCtt                                                                                                     >  1:292126/1‑139 (MQ=255)                                  ggCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTc                                                                                                   >  2:873866/1‑139 (MQ=255)                                      ccTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGGTAATCACCGATGTTCGCACGCCCGCCCGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGTCCACGACTCATTCCCATACCTTTCGCaa                                                                                               >  2:713291/1‑139 (MQ=255)                                                 ggCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACg                                                                                    <  1:525915/139‑1 (MQ=255)                                                       cgtcgCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   >  1:825809/1‑102 (MQ=255)                                                       cgtcgCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  2:825809/102‑1 (MQ=255)                                                       cgtcgCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  2:589734/102‑1 (MQ=255)                                                       cgtcgCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   >  1:589734/1‑102 (MQ=255)                                                       cgtcgCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCa                                                                              >  1:817968/1‑139 (MQ=255)                                                            ccGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAAt                                                                         <  1:607470/139‑1 (MQ=255)                                                                aCCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCt                                                                     >  1:1078750/1‑139 (MQ=255)                                                                aCCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCt                                                                     >  1:494546/1‑139 (MQ=255)                                                                    aTGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACg                                                                                    >  1:327613/1‑120 (MQ=255)                                                                    aTGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACg                                                                                    <  2:327613/120‑1 (MQ=255)                                                                        tGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGccc                                                             <  2:755784/139‑1 (MQ=255)                                                                         gATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   >  1:596804/1‑84 (MQ=255)                                                                         gATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  2:596804/84‑1 (MQ=255)                                                                          aTAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCgg                                                           >  1:3613/1‑139 (MQ=255)                                                                           tAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGc                                                          >  2:575384/1‑139 (MQ=255)                                                                               cACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACg                                                                                    <  1:210319/109‑1 (MQ=255)                                                                               cACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACg                                                                                    >  2:210319/1‑109 (MQ=255)                                                                                aCCGAAGTTCGCACGCCCGCCAGAATCACTGGAGCCGCCAGCGGTAGCTCGACCTAGCGCa                                                                                                                                   >  1:1112316/1‑61 (MQ=255)                                                                                aCCGAAGTTCGCACGCCCGCCAGAATCACTGGAGCCGCCAGCGGTAGCTCGACCTAGCGCa                                                                                                                                   <  2:1112316/61‑1 (MQ=255)                                                                                aCCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCa                                                                                                                 <  2:271276/79‑1 (MQ=255)                                                                                aCCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCa                                                                                                                 >  1:271276/1‑79 (MQ=255)                                                                                        tCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCa                                                                                                                 >  1:717385/1‑71 (MQ=255)                                                                                        tCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCa                                                                                                                 <  2:717385/71‑1 (MQ=255)                                                                                         cGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   >  1:973541/1‑68 (MQ=255)                                                                                         cGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  2:973541/68‑1 (MQ=255)                                                                                         cGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGACAAGTGTCGCCTGCa                                            >  1:579618/1‑139 (MQ=255)                                                                                           cACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCGTTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGCCAAGTGTCGCCTGCAgg                                          <  1:640399/139‑1 (MQ=255)                                                                                             cgcccgccAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGAc                                        <  2:609551/139‑1 (MQ=255)                                                                                                ccgccAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACggg                                     >  2:823548/1‑139 (MQ=255)                                                                                                      gAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGcac                               <  1:575384/139‑1 (MQ=255)                                                                                                        aTCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGTCCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCGGGACGGGCAGCacac                             <  1:713291/139‑1 (MQ=255)                                                                                                        aTCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCacac                             <  2:204960/139‑1 (MQ=255)                                                                                                        aTCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCacac                             <  1:113735/139‑1 (MQ=255)                                                                                                        aTCACCGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCacac                             <  2:3613/139‑1 (MQ=255)                                                                                                             cGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   >  1:703201/1‑48 (MQ=255)                                                                                                             cGGAGCCGCCAGCGGTAGCTCGACCTTGCGCACTCGCTGACCACGACt                                                                                                                   <  2:703201/48‑1 (MQ=255)                                                                                                                                   aCCTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTg  >  2:703505/1‑139 (MQ=255)                                                                                                                                     cTTGCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGcaca                              <  1:633270/109‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      AAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTG  >  NC_000913/2215862‑2216130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑